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Journal of Cell Science, Vol 108, Issue 12 3807-3816, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
H Ernst, MK Zanin, D Everman and S Hoffman
Medical University of South Carolina, Department of Medicine, Charleston 29425, USA.
Chondroitin sulfate proteoglycans inhibit the adhesion of cells to extracellular matrix proteins that otherwise permit adhesion. Although proteoglycans are widely assumed to act by masking the other protein in a mixed substrate, recent studies suggest that proteoglycans inhibit adhesion through mechanisms initiated by their binding to specific cell surface receptors. To explore this issue, we developed a purification scheme to isolate proteoglycan aggregates, monomers, and core proteins. Two distinct adhesion assays were used to study the interaction of these proteoglycan preparations with human foreskin fibroblasts: the gravity assay in which cell attachment is stabilized by cell spreading, and the centrifugation assay in which spreading does not play a role. All proteoglycan preparations mediate adhesion in the centrifugation assay but not in the gravity assay. In the centrifugation assay, proteoglycan aggregates and monomers are considerably more active than other extracellular matrix proteins while proteoglycan core proteins are at least as active as other extracellular matrix proteins. Proteoglycan core proteins bind to cell-associated hyaluronic acid, but not to integrins. Using mixed substrates in the gravity assay, all proteoglycan preparations inhibited cell attachment to fibronectin and vitronectin but not to collagen I and laminin. Although proteoglycan aggregates and monomers are more active than core proteins in inhibiting adhesion in the gravity assay, core proteins are still clearly active. A variety of control experiments suggest that the inhibition of cell attachment by proteoglycans is mediated through the specific interactions of proteoglycans with cell surface receptors, resulting in the inhibition of cell spreading. These results suggest at least two molecular mechanisms for proteoglycan-fibroblast interactions, one involving the chondroitin sulfate on the proteoglycan and an as yet unidentified receptor, the other involving the proteoglycan core protein and cell-associated hyaluronic acid.
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  H. Takagi, Y. Asano, N. Yamakawa, I. Matsumoto, and K. Kimata Annexin 6 is a putative cell surface receptor for chondroitin sulfate chains J. Cell Sci., August 15, 2002; 115(16): 3309 - 3318. [Abstract] [Full Text] [PDF]
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M. Stigson, J. Lofberg, and L. Kjellen PG-M/Versican-like Proteoglycans Are Components of Large Disulfide-stabilized Complexes in the Axolotl Embryo J. Biol. Chem., February 7, 1997; 272(6): 3246 - 3253. [Abstract] [Full Text] [PDF]
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