spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by White, M. R.
Right arrow Articles by Kingsman, S. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by White, M. R.
Right arrow Articles by Kingsman, S. M.

Journal of Cell Science, Vol 108, Issue 2 441-455, Copyright © 1995 by Company of Biologists

JOURNAL ARTICLES

Real-time analysis of the transcriptional regulation of HIV and hCMV promoters in single mammalian cells

MR White, M Masuko, L Amet, G Elliott, M Braddock, AJ Kingsman and SM Kingsman
Amersham International, Bucks, UK.

The regulation of human cytomegalovirus (hCMV) and human immunodeficiency virus (HIV) gene expression has been studied in single intact mammalian cells. Viral promoters were placed upstream of the firefly luciferase reporter gene and the resulting hybrid reporter constructs were stably integrated into the HeLa cell genome. A highly sensitive photon-counting camera system was used to study the level of gene expression in single intact cells. Luciferase expression was studied in the absence of activators of viral gene expression, in the presence of the HIV-1 TAT transactivator protein, or in the presence of sodium butyrate, a non-viral activator of gene expression. In the absence of any activator of gene expression, while expression was undetectable in most cells, significant levels of basal luciferase activity were observed in a few cells, indicating heterogeneity in gene expression in the cell population. In the presence of the general activator of viral gene expression, sodium butyrate, transcriptional activation from the viral promoters gave rise to significant and relatively homogeneous levels of luciferase expression in a majority of cells. The luciferase imaging technology was used for the real-time analysis of changes of gene expression within a single cell. This non-invasive reporter assay should become important for studies of the temporal regulation of gene expression in single cells.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
K. Gee, J. B. Angel, W. Ma, S. Mishra, N. Gajanayaka, K. Parato, and A. Kumar
Intracellular HIV-Tat Expression Induces IL-10 Synthesis by the CREB-1 Transcription Factor through Ser133 Phosphorylation and Its Regulation by the ERK1/2 MAPK in Human Monocytic Cells
J. Biol. Chem., October 20, 2006; 281(42): 31647 - 31658.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Lesner, Y. Li, J. Nitkiewicz, G. Li, A. Kartvelishvili, M. Kartvelishvili, and M. Simm
A Soluble Factor Secreted by an HIV-1-Resistant Cell Line Blocks Transcription through Inactivating the DNA-Binding Capacity of the NF-{kappa}B p65/p50 Dimer
J. Immunol., August 15, 2005; 175(4): 2548 - 2554.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
C. T. French, W. H. Hanneman, L. S. Chubb, R. E. Billings, and M. E. Andersen
Induction of CYP1A1 in Primary Rat Hepatocytes by 3,3',4,4',5-Pentachlorobiphenyl: Evidence for a Switch Circuit Element
Toxicol. Sci., April 1, 2004; 78(2): 276 - 286.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
A. J. Norris, J. A. Stirland, D. W. McFerran, Z. C. Seymour, D. G. Spiller, A. S. I. Loudon, M. R. H. White, and J. R. E. Davis
Dynamic Patterns of Growth Hormone Gene Transcription in Individual Living Pituitary Cells
Mol. Endocrinol., February 1, 2003; 17(2): 193 - 202.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
D. W. McFerran, J. A. Stirland, A. J. Norris, R. A. Khan, N. Takasuka, Z. C. Seymour, M. S. Gill, W. R. Robertson, A. S. I. Loudon, J. R. E. Davis, et al.
Persistent Synchronized Oscillations in Prolactin Gene Promoter Activity in Living Pituitary Cells
Endocrinology, July 1, 2001; 142(7): 3255 - 3260.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
D. A. Hume
Probability in transcriptional regulation and its implications for leukocyte differentiation and inducible gene expression
Blood, October 1, 2000; 96(7): 2323 - 2328.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Faria, C. D. Wood, L. Perrouault, J. S. Nelson, A. Winter, M. R. H. White, C. Helene, and C. Giovannangeli
Targeted inhibition of transcription elongation in cells mediated by triplex-forming oligonucleotides
PNAS, April 11, 2000; 97(8): 3862 - 3867.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
S. Newlands, L. K. Levitt, C. S. Robinson, A.B. C. Karpf, V. R.M. Hodgson, R. P. Wade, and E. C. Hardeman
Transcription occurs in pulses in muscle fibers
Genes & Dev., September 1, 1998; 12(17): 2748 - 2758.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
N. Takasuka, M. R. H. White, C. D. Wood, W. R. Robertson, and J. R. E. Davis
Dynamic Changes in Prolactin Promoter Activation in Individual Living Lactotrophic Cells
Endocrinology, March 1, 1998; 139(3): 1361 - 1368.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1995