|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 108, Issue 2 821-829, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
C Wu, AJ Fields, BA Kapteijn and JA McDonald
Samule C. Johnson Medical Research Center, Mayo Clinic Scottsdale, AZ 85259, USA.
The alpha 4 beta 1 integrin has been suggested to play important roles in embryogenesis and pathogenesis of many diseases which involve both cell adhesion and cell migration. Previous studies using anti-alpha 4 beta 1 antibodies and fibronectin (Fn) fragments have suggested that alpha 4 beta 1 integrins may be involved in cell motility on Fn and vascular cell adhesion molecule-1 (VCAM-1). However, the cells used in these studies also express other Fn integrin receptors including alpha 5 beta 1 integrin, which is known to function in cell motility on Fn. To test whether alpha 4 beta 1 integrins mediate cell motility on Fn and VCAM-1 in the absence of alpha 5 beta 1 integrin, we expressed human alpha 4 integrin in a Chinese hamster ovary (CHO) cell line that is deficient in alpha 5 beta 1 integrin (CHO B2). The parental alpha 5 deficient CHO B2 cells were unable to adhere, spread or migrate on Fn, nor could they assemble a fibrillar Fn matrix. Expression of alpha 4 beta 1 integrin in the CHO B2 cells enabled the cells to adhere, spread and migrate on Fn and on VCAM-1 but not to assemble a fibrillar Fn matrix. The cellular processes mediated by the interaction of alpha 4 beta 1 with Fn or VCAM-1 were inhibited by the CS1 peptide derived from the major alpha 4 beta 1 binding site on Fn. These findings demonstrate that alpha 4 beta 1 integrins not only function as cell adhesion receptors but also as cell motility receptors for Fn and VCAM-1 independent of alpha 5 beta 1. Moreover, they reveal important functional differences between Fn binding integrins. The alpha 4-positive, alpha 5-negative CHO cells described in this report will be useful tools in studying the mechanism of molecular signalling during integrin mediated cellular processes.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  C. C. Feral, A. Zijlstra, E. Tkachenko, G. Prager, M. L. Gardel, M. Slepak, and M. H. Ginsberg CD98hc (SLC3A2) participates in fibronectin matrix assembly by mediating integrin signaling J. Cell Biol., August 9, 2007; 178(4): 701 - 711. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Bhasin, P. Carl, S. Harper, G. Feng, H. Lu, D. W. Speicher, and D. E. Discher Chemistry on a Single Protein, Vascular Cell Adhesion Molecule-1, during Forced Unfolding J. Biol. Chem., October 29, 2004; 279(44): 45865 - 45874. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Zaniolo, S. Leclerc, A. Cvekl, L. Vallieres, R. Bazin, K. Larouche, and S. L. Guerin Expression of the {alpha}4 Integrin Subunit Gene Promoter Is Modulated by the Transcription Factor Pax-6 in Corneal Epithelial Cells Invest. Ophthalmol. Vis. Sci., June 1, 2004; 45(6): 1692 - 1704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Fukuda, K. Chen, X. Shi, and C. Wu PINCH-1 Is an Obligate Partner of Integrin-linked Kinase (ILK) Functioning in Cell Shape Modulation, Motility, and Survival J. Biol. Chem., December 19, 2003; 278(51): 51324 - 51333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Segerman, J. P. Atkinson, M. Marttila, V. Dennerquist, G. Wadell, and N. Arnberg Adenovirus Type 11 Uses CD46 as a Cellular Receptor J. Virol., September 1, 2003; 77(17): 9183 - 9191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Na, M. Marsden, and D. W. DeSimone Differential regulation of cell adhesive functions by integrin {alpha} subunit cytoplasmic tails in vivo J. Cell Sci., June 1, 2003; 116(11): 2333 - 2343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Pinco, W. He, and J. T. Yang alpha 4beta 1 Integrin Regulates Lamellipodia Protrusion via a Focal Complex/Focal Adhesion-independent Mechanism Mol. Biol. Cell, September 1, 2002; 13(9): 3203 - 3217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Cunningham, J. M. Rodriguez, M. P. Arrate, T. M. Tran, and T. A. Brock JAM2 Interacts with alpha 4beta 1. FACILITATION BY JAM3 J. Biol. Chem., July 26, 2002; 277(31): 27589 - 27592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Michaelson, J. D. Ritzenthaler, and J. Roman Regulation of serum-induced fibronectin expression by protein kinases, cytoskeletal integrity, and CREB Am J Physiol Lung Cell Mol Physiol, February 1, 2002; 282(2): L291 - L301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Tu, Y. Huang, Y. Zhang, Y. Hua, and C. Wu A New Focal Adhesion Protein that Interacts with Integrin-linked Kinase and Regulates Cell Adhesion and Spreading J. Cell Biol., April 30, 2001; 153(3): 585 - 598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sechler, A. Cumiskey, D. Gazzola, and J. Schwarzbauer A novel RGD-independent fibronectin assembly pathway initiated by alpha4beta1 integrin binding to the alternatively spliced V region J. Cell Sci., January 4, 2000; 113(8): 1491 - 1498. [Abstract] [PDF]
|
|
|
|
|
|
C. Klass, J. Couchman, and A Woods Control of extracellular matrix assembly by syndecan-2 proteoglycan J. Cell Sci., January 2, 2000; 113(3): 493 - 506. [Abstract] [PDF]
|
|
|
|
|
|
S Testaz, M Delannet, and J Duband Adhesion and migration of avian neural crest cells on fibronectin require the cooperating activities of multiple integrins of the (beta)1 and (beta)3 families J. Cell Sci., January 12, 1999; 112(24): 4715 - 4728. [Abstract] [PDF]
|
|
|
|
|
|
Q. Zhang, T. Sakai, J. Nowlen, I. Hayashi, R. Fassler, and D. F. Mosher Functional beta 1-Integrins Release the Suppression of Fibronectin Matrix Assembly by Vitronectin J. Biol. Chem., January 1, 1999; 274(1): 368 - 375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bourdoulous, G. Orend, D. A. MacKenna, R. Pasqualini, and E. Ruoslahti Fibronectin Matrix Regulates Activation of RHO and CDC42 GTPases and Cell Cycle Progression J. Cell Biol., October 5, 1998; 143(1): 267 - 276. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Magnusson and D. F. Mosher Fibronectin : Structure, Assembly, and Cardiovascular Implications Arterioscler. Thromb. Vasc. Biol., September 1, 1998; 18(9): 1363 - 1370. [Full Text] [PDF]
|
|
|
|
|
|
C. Wu, S. Y. Keightley, C. Leung-Hagesteijn, G. Radeva, M. Coppolino, S. Goicoechea, J. A. McDonald, and S. Dedhar Integrin-linked Protein Kinase Regulates Fibronectin Matrix Assembly, E-cadherin Expression, and Tumorigenicity J. Biol. Chem., January 2, 1998; 273(1): 528 - 536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. O. Simon, E. M. Nutt, D. G. Abraham, G. A. Rodan, and L. T. Duong The alpha vbeta 3 Integrin Regulates alpha 5beta 1-mediated Cell Migration toward Fibronectin J. Biol. Chem., November 14, 1997; 272(46): 29380 - 29389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Yao, B. Ziober, A. Sutherland, D. Mendrick, and R. Kramer Laminins promote the locomotion of skeletal myoblasts via the alpha 7 integrin receptor J. Cell Sci., January 12, 1996; 109(13): 3139 - 3150. [Abstract] [PDF]
|
|
|
|
|
|
C Wu, A. Chung, and J. McDonald A novel role for alpha 3 beta 1 integrins in extracellular matrix assembly J. Cell Sci., January 6, 1995; 108(6): 2511 - 2523. [Abstract] [PDF]
|
|
