|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 108, Issue 3 1003-1016, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
SC Kiley and PJ Parker
Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, London, UK.
U937 human promonocytic leukemia cells express PKC isozymes beta 1, beta 2, epsilon and zeta. Indirect immunocytofluorescence using affinity-purified PKC-specific antibodies indicates that each of the endogenous PKC isozymes in U937 cells display a unique compartmentalization within the intact cell. PKC-beta 1 is distributed between two identifiable pools: a cytoplasmic pool which redistributes to the plasma membrane upon activation with acute phorbol ester-treatment, and a membrane-bound pool associated with intracellular vesicles containing beta 2-integrin adhesion molecules, cd11b and cd11c. The vesicle-associated PKC-beta 1 translocates with the secretory granules to the plasma membrane upon agonist-stimulated activation. PKC-beta 2 is associated with the microtubule cytoskeleton in resting cells. PKC overlay assays indicate that PKC-beta 2 binds to proteins associated with microtubules, and not directly to tubulin. PKC-epsilon is associated with filamentous structures in resting cells and redistributes to the perinuclear region upon activation with phorbol esters. In differentiated U937 cells, PKC-beta 1 remains associated with vesicles translocating from the trans-Golgi region to the plasma membrane and PKC-epsilon is primarily associated with perinuclear and plasma membranes. PKC-zeta, which does not respond to phorbol ester treatment, is primarily cytosolic in undifferentiated cells and accumulates in the nucleus of differentiated cells blocked in the G2 phase of the cell cycle. The data clearly demonstrate that individual PKCs localize to different subcellular compartments and promote the hypothesis that PKC subcellular localization is indicative of unique functions for individual PKC isozymes.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Kim, Y.-L. Choi, A. Vallentin, B. S. Hunrichs, M. K. Hellerstein, D. M. Peehl, and D. Mochly-Rosen Centrosomal PKC{beta}II and Pericentrin Are Critical for Human Prostate Cancer Growth and Angiogenesis Cancer Res., August 15, 2008; 68(16): 6831 - 6839. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Togo Disruption of the plasma membrane stimulates rearrangement of microtubules and lipid traffic toward the wound site J. Cell Sci., July 1, 2006; 119(13): 2780 - 2786. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. J. Green, P. Marder, C. Ray, C. A. Cook, S. Jaken, L. C. Musib, R. S. Herbst, M. Carducci, C. D. Britten, M. Basche, et al. Development and Validation of a Drug Activity Biomarker that Shows Target Inhibition in Cancer Patients Receiving Enzastaurin, a Novel Protein Kinase C-{beta} Inhibitor. Clin. Cancer Res., June 1, 2006; 12(11): 3408 - 3415. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Can, O. Semiz, and O. Cinar Bisphenol-A induces cell cycle delay and alters centrosome and spindle microtubular organization in oocytes during meiosis Mol. Hum. Reprod., June 1, 2005; 11(6): 389 - 396. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Chen, A. Purohit, E. Halilovic, S. J. Doxsey, and A. C. Newton Centrosomal Anchoring of Protein Kinase C {beta}II by Pericentrin Controls Microtubule Organization, Spindle Function, and Cytokinesis J. Biol. Chem., February 6, 2004; 279(6): 4829 - 4839. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Chibber, B. M. Ben-Mahmud, G. E. Mann, J. J. Zhang, and E. M. Kohner Protein Kinase C {beta}2-Dependent Phosphorylation of Core 2 GlcNAc-T Promotes Leukocyte-Endothelial Cell Adhesion: A Mechanism Underlying Capillary Occlusion in Diabetic Retinopathy Diabetes, June 1, 2003; 52(6): 1519 - 1527. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-S. Choi, D. Wang, J. Dadgar, W. S. Chang, and R. O. Messing Conditional Rescue of Protein Kinase C epsilon Regulates Ethanol Preference and Hypnotic Sensitivity in Adult Mice J. Neurosci., November 15, 2002; 22(22): 9905 - 9911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nakhost, N. Kabir, P. Forscher, and W. S. Sossin Protein Kinase C Isoforms Are Translocated to Microtubules in Neurons J. Biol. Chem., October 18, 2002; 277(43): 40633 - 40639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Blass, I. Kronfeld, G. Kazimirsky, P. M. Blumberg, and C. Brodie Tyrosine Phosphorylation of Protein Kinase C{delta} Is Essential for Its Apoptotic Effect in Response to Etoposide Mol. Cell. Biol., January 1, 2002; 22(1): 182 - 195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L Leaney, L. V Dekker, and A. Tinker Regulation of a G protein-gated inwardly rectifying K+ channel by a Ca2+-independent protein kinase C J. Physiol., July 15, 2001; 534(2): 367 - 379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. Greenaway, F. M. Cunningham, and N. T. Goode Differential localization of protein kinase C isotypes in equine eosinophils and neutrophils J. Leukoc. Biol., October 1, 2000; 68(4): 575 - 582. [Abstract] [Full Text]
|
|
|
|
|
|
N. J. Brandon, J. M. Uren, J. T. Kittler, H. Wang, R. Olsen, P. J. Parker, and S. J. Moss Subunit-Specific Association of Protein Kinase C and the Receptor for Activated C Kinase with GABA Type A Receptors J. Neurosci., November 1, 1999; 19(21): 9228 - 9234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Kiley, K. J. Clark, M. Goodnough, D. R. Welch, and S. Jaken Protein Kinase C {{delta}} Involvement in Mammary Tumor Cell Metastasis Cancer Res., July 1, 1999; 59(13): 3230 - 3238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-F. Yan, J. Lu, Y. S. Zou, J. Soh-Won, D. M. Cohen, P. M. Buttrick, D. R. Cooper, S. F. Steinberg, N. Mackman, D. J. Pinsky, et al. Hypoxia-associated Induction of Early Growth Response-1 Gene Expression J. Biol. Chem., May 21, 1999; 274(21): 15030 - 15040. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. He, X. Wang, M. Gorospe, N. J. Holbrook, and M. A. Trush Phorbol Ester-induced Mononuclear Cell Differentiation Is Blocked by the Mitogen-activated Protein Kinase Kinase (MEK) Inhibitor PD98059 Cell Growth Differ., May 1, 1999; 10(5): 307 - 315. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. P. HATTON, F. GAUBERT, M. L. LEWIS, Y. DARSEL, P. OHLMANN, J.-P. CAZENAVE, and D. SCHMITT The kinetics of translocation and cellular quantity of protein kinase C in human leukocytes are modified during spaceflight FASEB J, May 1, 1999; 13(9001): 23 - 33. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. H. Whelan, S. C. Kiley, and P. J. Parker Tetradecanoyl Phorbol Acetate-induced Microtubule Reorganization Is Required for Sustained Mitogen-activated Protein Kinase Activation and Morphological Differentiation of U937 Cells Cell Growth Differ., April 1, 1999; 10(4): 271 - 277. [Abstract] [Full Text]
|
|
|
|
|
|
O. P. Barry, M. G. Kazanietz, D. Pratico, and G. A. FitzGerald Arachidonic Acid in Platelet Microparticles Up-regulates Cyclooxygenase-2-dependent Prostaglandin Formation via a Protein Kinase C/Mitogen-activated Protein Kinase-dependent Pathway J. Biol. Chem., March 12, 1999; 274(11): 7545 - 7556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Volkov, A. Long, and D. Kelleher Inside the Crawling T Cell: Leukocyte Function-Associated Antigen-1 Cross-Linking Is Associated with Microtubule-Directed Translocation of Protein Kinase C Isoenzymes {beta}(I) and {delta} J. Immunol., December 15, 1998; 161(12): 6487 - 6495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Mayne and A. W. Murray Evidence That Protein Kinase Cepsilon Mediates Phorbol Ester Inhibition of Calphostin C- and Tumor Necrosis Factor-alpha -induced Apoptosis in U937 Histiocytic Lymphoma Cells J. Biol. Chem., September 11, 1998; 273(37): 24115 - 24121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Lewis, J. L. Reynolds, L. A. Cubano, J. P. Hatton, B. D. Lawless, and E. H. Piepmeier Spaceflight alters microtubules and increases apoptosis in human lymphocytes (Jurkat) FASEB J, August 1, 1998; 12(11): 1007 - 1018. [Abstract] [Full Text]
|
|
|
|
|
|
X. Feng, J. Zhang, L. S. Barak, T. Meyer, M. G. Caron, and Y. A. Hannun Visualization of Dynamic Trafficking of a Protein Kinase C beta II/Green Fluorescent Protein Conjugate Reveals Differences in G Protein-coupled Receptor Activation and Desensitization J. Biol. Chem., April 24, 1998; 273(17): 10755 - 10762. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Haller, C. Lindschau, C. Maasch, H. Olthoff, D. Kurscheid, and F. C. Luft Integrin-Induced Protein Kinase C{alpha} and C{epsilon} Translocation to Focal Adhesions Mediates Vascular Smooth Muscle Cell Spreading Circ. Res., February 9, 1998; 82(2): 157 - 165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-L. Wu, J. Staudinger, E. N. Olson, and C. S. Rubin Structure, Expression, and Properties of an Atypical Protein Kinase C (PKC3) from Caenorhabditis elegans. PKC3 IS REQUIRED FOR THE NORMAL PROGRESSION OF EMBRYOGENESIS AND VIABILITY OF THE ORGANISM J. Biol. Chem., January 9, 1998; 273(2): 1130 - 1143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Lorenzo, K. Bogi, P. Acs, G. R. Pettit, and P. M. Blumberg The Catalytic Domain of Protein Kinase Cdelta Confers Protection from Down-regulation Induced by Bryostatin 1 J. Biol. Chem., December 26, 1997; 272(52): 33338 - 33343. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Zhou, M. L. Seibenhener, and M. W. Wooten Nucleolin Is a Protein Kinase C-zeta Substrate. CONNECTION BETWEEN CELL SURFACE SIGNALING AND NUCLEUS IN PC12 CELLS J. Biol. Chem., December 5, 1997; 272(49): 31130 - 31137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Acs, K. Bogi, P. S. Lorenzo, A. M. Marquez, T. Biro, Z. Szallasi, and P. M. Blumberg The Catalytic Domain of Protein Kinase C Chimeras Modulates the Affinity and Targeting of Phorbol Ester-induced Translocation J. Biol. Chem., August 29, 1997; 272(35): 22148 - 22153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Herrera-Velit, K. L. Knutson, and N. E. Reiner Phosphatidylinositol 3-Kinase-dependent Activation of Protein Kinase C-zeta in Bacterial Lipopolysaccharide-treated Human Monocytes J. Biol. Chem., June 27, 1997; 272(26): 16445 - 16452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Hundle, T. McMahon, J. Dadgar, C.-H. Chen, D. Mochly-Rosen, and R. O. Messing An Inhibitory Fragment Derived from Protein Kinase Cepsilon Prevents Enhancement of Nerve Growth Factor Responses by Ethanol and Phorbol Esters J. Biol. Chem., June 6, 1997; 272(23): 15028 - 15035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Canman and W. Bement Microtubules suppress actomyosin-based cortical flow in Xenopus oocytes J. Cell Sci., January 8, 1997; 110(16): 1907 - 1917. [Abstract] [PDF]
|
|
|
|
|
|
C. Chapline, B. Mousseau, K. Ramsay, S. Duddy, Y. Li, S. C. Kiley, and S. Jaken Identification of a Major Protein Kinase C-binding Protein and Substrate in Rat Embryo Fibroblasts J. Biol. Chem., March 15, 1996; 271(11): 6417 - 6422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. E. Faulkner, J. M. Hilfinger, and D. M. Markovitz Protein Phosphatase 2A Activates the HIV-2 Promoter through Enhancer Elements That Include the pets Site J. Biol. Chem., July 6, 2001; 276(28): 25804 - 25812. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. S. Niino, T. Irie, M. Takaishi, T. Hosono, N.-h. Huh, T. Tachikawa, and T. Kuroki PKCtheta II, a New Isoform of Protein Kinase C Specifically Expressed in the Seminiferous Tubules of Mouse Testis J. Biol. Chem., September 21, 2001; 276(39): 36711 - 36717. [Abstract] [Full Text] [PDF]
|
|
