spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moscatelli, A.
Right arrow Articles by Cresti, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moscatelli, A.
Right arrow Articles by Cresti, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 108, Issue 3 1117-1125, Copyright © 1995 by Company of Biologists

JOURNAL ARTICLES

High molecular weight polypeptides related to dynein heavy chains in Nicotiana tabacum pollen tubes

A Moscatelli, C Del Casino, L Lozzi, G Cai, M Scali, A Tiezzi and M Cresti
Dipartimento di Biologia Ambientale, Universita di Siena, Italia.

Nicotiana tabacum pollen tubes contain two high molecular weight polypeptides (about 400 kDa), which are specifically expressed during pollen germination and pollen tube growth in BK medium. The high molecular weight doublet resembles the dynein heavy chains in some biochemical properties. Sedimentation profiles of pollen tube extracts show that the high molecular weight bands have sedimentation coefficients of 22 S and 12 S, respectively. ATPase assay of sedimentation fractions shows an activity ten times higher when stimulated by the presence of bovine brain microtubules in fractions containing the 22 S high molecular weight polypeptide. Both these high molecular weight polypeptides can bind microtubules in an ATP-dependent fashion. A mouse antiserum to a synthetic peptide reproducing the sequence of the most conserved ATP-binding site among dynein heavy chains recognized the two high molecular weight polypeptides. Therefore these polypeptides have sequences immunologically related to the ATP binding sites of dynein heavy chains.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
G. Cai and M. Cresti
Organelle motility in the pollen tube: a tale of 20 years
J. Exp. Bot., February 1, 2009; 60(2): 495 - 508.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S. Romagnoli, G. Cai, C. Faleri, E. Yokota, T. Shimmen, and M. Cresti
Microtubule- and Actin Filament-Dependent Motors are Distributed on Pollen Tube Mitochondria and Contribute Differently to Their Movement
Plant Cell Physiol., February 1, 2007; 48(2): 345 - 361.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Romagnoli, G. Cai, and M. Cresti
In Vitro Assays Demonstrate That Pollen Tube Organelles Use Kinesin-Related Motor Proteins to Move along Microtubules
PLANT CELL, January 1, 2003; 15(1): 251 - 269.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
I. B. Heath and A. Geitmann
Cell Biology of Plant and Fungal Tip Growth--Getting to the Point
PLANT CELL, September 1, 2000; 12(9): 1513 - 1517.
[Full Text]

Home page
 Plant CellHome page
G. Cai, S. Romagnoli, A. Moscatelli, E. Ovidi, G. Gambellini, A. Tiezzi, and M. Cresti
Identification and Characterization of a Novel Microtubule-Based Motor Associated with Membranous Organelles in Tobacco Pollen Tubes
PLANT CELL, September 1, 2000; 12(9): 1719 - 1736.
[Abstract] [Full Text]


© The Company of Biologists Ltd 1995