spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Horvat, R.
Right arrow Articles by Palade, G. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Horvat, R.
Right arrow Articles by Palade, G. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 108, Issue 3 1155-1164, Copyright © 1995 by Company of Biologists

JOURNAL ARTICLES

The functional thrombin receptor is associated with the plasmalemma and a large endosomal network in cultured human umbilical vein endothelial cells

R Horvat and GE Palade
University of California, San Diego, School of Medicine, Division of Cellular and Molecular Medicine 92093-0651, USA.

The functional thrombin receptor, normally expressed by endothelial cells and platelets, is a member of the G protein-coupled, seven membrane-spanning-domain receptor family and is thought to be responsible for most, if not all, the cell stimulatory effects of thrombin. Upon binding, thrombin cleaves the receptor's N-terminal ectodomain, unmasking a new N terminus, which by itself activates the receptor. Using antibodies to different domains of the human thrombin receptor, we have localized the receptor in cultured human umbilical vein endothelial cells by indirect immunofluorescence and immunoelectron microscopy. We found the receptor expressed on the plasmalemma of cultured endothelial cells in individual units rather than in clusters, at lower concentration than, and at different sites from, thrombomodulin. We also found the receptor associated with a distinct, intracellular, transferrin receptor-containing, tubulovesicular network. The thrombin receptor-positive structure spread from the perinuclear region to the periphery of the cells, exhibiting a number of varicosities interconnected by branching tubular elements, strikingly similar to an image recently described for a continuous endosomal reticulum. Our results provide morphological evidence for the presence of the functional thrombin receptor at relative low density on the surface of cultured endothelial cells (compared to thrombomodulin) and in relatively large quantities inside the cells, associated with an endosomal compartment.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Carlile-Klusacek and V. Rizzo
Endothelial cytoskeletal reorganization in response to PAR1 stimulation is mediated by membrane rafts but not caveolae
Am J Physiol Heart Circ Physiol, July 1, 2007; 293(1): H366 - H375.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. M. Paing, C. A. Johnston, D. P. Siderovski, and J. Trejo
Clathrin Adaptor AP2 Regulates Thrombin Receptor Constitutive Internalization and Endothelial Cell Resensitization
Mol. Cell. Biol., April 15, 2006; 26(8): 3231 - 3242.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
D. Mehta and A. B. Malik
Signaling Mechanisms Regulating Endothelial Permeability
Physiol Rev, January 1, 2006; 86(1): 279 - 367.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
V. S. OSSOVSKAYA and N. W. BUNNETT
Protease-Activated Receptors: Contribution to Physiology and Disease
Physiol Rev, April 1, 2004; 84(2): 579 - 621.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. A. Ellis, A. B. Malik, A. Gilchrist, H. Hamm, R. Sandoval, T. Voyno-Yasenetskaya, and C. Tiruppathi
Thrombin Induces Proteinase-activated Receptor-1 Gene Expression in Endothelial Cells via Activation of Gi-linked Ras/Mitogen-activated Protein Kinase Pathway
J. Biol. Chem., May 7, 1999; 274(19): 13718 - 13727.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K Ihida, D Predescu, R. Czekay, and G. Palade
Platelet activating factor receptor (PAF-R) is found in a large endosomal compartment in human umbilical vein endothelial cells
J. Cell Sci., January 2, 1999; 112(3): 285 - 295.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. A. Ellis, C. Tiruppathi, R. Sandoval, W. D. Niles, and A. B. Malik
Time course of recovery of endothelial cell surface thrombin receptor (PAR-1) expression
Am J Physiol Cell Physiol, January 1, 1999; 276(1): C38 - C45.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. H. Creer and J. McHowat
Selective hydrolysis of plasmalogens in endothelial cells following thrombin stimulation
Am J Physiol Cell Physiol, December 1, 1998; 275(6): C1498 - C1507.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. J. Shapiro and S. R. Coughlin
Separate Signals for Agonist-independent and Agonist-triggered Trafficking of Protease-activated Receptor 1
J. Biol. Chem., October 30, 1998; 273(44): 29009 - 29014.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
O. Dery, C. U. Corvera, M. Steinhoff, and N. W. Bunnett
Proteinase-activated receptors: novel mechanisms of signaling by serine proteases
Am J Physiol Cell Physiol, June 1, 1998; 274(6): C1429 - C1452.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
M. Molino, P. N. Raghunath, A. Kuo, M. Ahuja, J. A. Hoxie, L. F. Brass, and E. S. Barnathan
Differential Expression of Functional Protease-Activated Receptor-2 (PAR-2) in Human Vascular Smooth Muscle Cells
Arterioscler Thromb Vasc Biol, May 1, 1998; 18(5): 825 - 832.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Molino, M. J. Woolkalis, J. Reavey-Cantwell, D. Pratico, P. Andrade-Gordon, E. S. Barnathan, and L. F. Brass
Endothelial Cell Thrombin Receptors and PAR-2. TWO PROTEASE-ACTIVATED RECEPTORS LOCATED IN A SINGLE CELLULAR ENVIRONMENT
J. Biol. Chem., April 25, 1997; 272(17): 11133 - 11141.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Molino, D. F. Bainton, J. A. Hoxie, S. R. Coughlin, and L. F. Brass
Thrombin Receptors on Human Platelets. INITIAL LOCALIZATION AND SUBSEQUENT REDISTRIBUTION DURING PLATELET ACTIVATION
J. Biol. Chem., February 28, 1997; 272(9): 6011 - 6017.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. J. Shapiro, J. Trejo, D. Zeng, and S. R. Coughlin
Role of the Thrombin Receptor's Cytoplasmic Tail in Intracellular Trafficking. DISTINCT DETERMINANTS FOR AGONIST-TRIGGERED VERSUS TONIC INTERNALIZATION AND INTRACELLULAR LOCALIZATION
J. Biol. Chem., December 20, 1996; 271(51): 32874 - 32880.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. K. Bohm, L. M. Khitin, E. F. Grady, G. Aponte, D. G. Payan, and N. W. Bunnett
Mechanisms of Desensitization and Resensitization of Proteinase-activated Receptor-2
J. Biol. Chem., September 6, 1996; 271(36): 22003 - 22016.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Nystedt, V. Ramakrishnan, and J. Sundelin
The Proteinase-activated Receptor 2Is Induced by Inflammatory Mediators in Human Endothelial Cells. COMPARISON WITH THE THROMBIN RECEPTOR
J. Biol. Chem., June 21, 1996; 271(25): 14910 - 14915.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1995