|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 108, Issue 4 1629-1637, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
D Tuckwell, DA Calderwood, LJ Green and MJ Humphries
School of Biological Sciences, University of Manchester, UK.
Integrins alpha 1 beta 1 and alpha 2 beta 1 are major cellular receptors for collagens. The alpha 1 and alpha 2 subunits contain a approximately 200 amino acid inserted domain (I-domain) in their N-terminal region and, because of the homology between the I-domains and the collagen-binding A-domains of von Willebrand factor, it has been suggested that the I-domains might mediate the collagen-binding functions of alpha 1 beta 1 and alpha 2 beta 1. In order to fully investigate this hypothesis, we have generated recombinant human alpha 2 I-domain (r alpha 2I) by reverse transcriptase-polymerase chain reaction/bacterial expression and tested its ability to mediate the collagen-binding functions of alpha 2 beta 1. R alpha 2 I binds specifically to type I collagen in a concentration-dependent manner: binding is cation dependent and, like the complete receptor, is supported by magnesium and manganese ions but not by calcium ions. R alpha 2I is recognised by anti-functional anti-alpha 2 monoclonal antibodies 6F1, 5E8 and P1E6 in capture ELISAs, and anti-functional antibodies inhibited r alpha 2I-collagen binding. In addition, r alpha 2I inhibits cell spreading on collagen. R alpha 2I is therefore a collagen-binding domain and can account for many of the collagen-binding functions of integrin alpha 2 beta 1. We have also determined the collagen specificity of r alpha 2I and found that it binds types I, II and XI collagen.
This article has been cited by other articles:
|
|
 |
|
  D. Valdramidou, M. J. Humphries, and A. P. Mould Distinct Roles of {beta}1 Metal Ion-dependent Adhesion Site (MIDAS), Adjacent to MIDAS (ADMIDAS), and Ligand-associated Metal-binding Site (LIMBS) Cation-binding Sites in Ligand Recognition by Integrin {alpha}2{beta}1 J. Biol. Chem., November 21, 2008; 283(47): 32704 - 32714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. R. Fiedler, E. Schonherr, R. Waddington, S. Niland, D. G. Seidler, D. Aeschlimann, and J. A. Eble Decorin Regulates Endothelial Cell Motility on Collagen I through Activation of Insulin-like Growth Factor I Receptor and Modulation of {alpha}2{beta}1 Integrin Activity J. Biol. Chem., June 20, 2008; 283(25): 17406 - 17415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Lambert, A. A. Bobkov, J. W. Smith, and F. M. Marassi Competitive Interactions of Collagen and a Jararhagin-derived Disintegrin Peptide with the Integrin {alpha}2-I Domain J. Biol. Chem., June 13, 2008; 283(24): 16665 - 16672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Taubenberger, D. A. Cisneros, J. Friedrichs, P.-H. Puech, D. J. Muller, and C. M. Franz Revealing Early Steps of {alpha}2beta1 Integrin-mediated Adhesion to Collagen Type I by Using Single-Cell Force Spectroscopy Mol. Biol. Cell, May 1, 2007; 18(5): 1634 - 1644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Eble, A. Kassner, S. Niland, M. Morgelin, J. Grifka, and S. Grassel Collagen XVI Harbors an Integrin {alpha}1beta1 Recognition Site in Its C-terminal Domains J. Biol. Chem., September 1, 2006; 281(35): 25745 - 25756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Raynal, S. W. Hamaia, P. R.-M. Siljander, B. Maddox, A. R. Peachey, R. Fernandez, L. J. Foley, D. A. Slatter, G. E. Jarvis, and R. W. Farndale Use of Synthetic Peptides to Locate Novel Integrin {alpha}2beta1-binding Motifs in Human Collagen III J. Biol. Chem., February 17, 2006; 281(7): 3821 - 3831. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Jokinen, E. Dadu, P. Nykvist, J. Kapyla, D. J. White, J. Ivaska, P. Vehvilainen, H. Reunanen, H. Larjava, L. Hakkinen, et al. Integrin-mediated Cell Adhesion to Type I Collagen Fibrils J. Biol. Chem., July 23, 2004; 279(30): 31956 - 31963. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Schoolmeester, K. Vanhoorelbeke, S. Katsutani, H. Depraetere, H. B. Feys, J. M. W. Heemskerk, M. F. Hoylaerts, and H. Deckmyn Monoclonal antibody IAC-1 is specific for activated {alpha}2{beta}1 and binds to amino acids 199 to 201 of the integrin {alpha}2 I-domain Blood, July 15, 2004; 104(2): 390 - 396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Perret, J. A. Eble, P. R.-M. Siljander, C. Merle, R. W. Farndale, M. Theisen, and F. Ruggiero Prolyl Hydroxylation of Collagen Type I Is Required for Efficient Binding to Integrin {alpha}1{beta}1 and Platelet Glycoprotein VI but Not to {alpha}2{beta}1 J. Biol. Chem., August 8, 2003; 278(32): 29873 - 29879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Romijn, E. Westein, B. Bouma, M. E. Schiphorst, J. J. Sixma, P. J. Lenting, and E. G. Huizinga Mapping the Collagen-binding Site in the von Willebrand Factor-A3 Domain J. Biol. Chem., April 18, 2003; 278(17): 15035 - 15039. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Zigrino, A. S. Kamiguti, J. Eble, C. Drescher, R. Nischt, J. W. Fox, and C. Mauch The Reprolysin Jararhagin, a Snake Venom Metalloproteinase, Functions as a Fibrillar Collagen Agonist Involved in Fibroblast Cell Adhesion and Signaling J. Biol. Chem., October 18, 2002; 277(43): 40528 - 40535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Smith, D. Estavillo, J. Emsley, L. A. Bankston, R. C. Liddington, and M. A. Cruz Mapping the Collagen-binding Site in the I Domain of the Glycoprotein Ia/IIa (Integrin alpha 2beta 1) J. Biol. Chem., February 11, 2000; 275(6): 4205 - 4209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. G. Knight, L. F. Morton, A. R. Peachey, D. S. Tuckwell, R. W. Farndale, and M. J. Barnes The Collagen-binding A-domains of Integrins alpha 1beta 1 and alpha 2beta 1 Recognize the Same Specific Amino Acid Sequence, GFOGER, in Native (Triple-helical) Collagens J. Biol. Chem., January 7, 2000; 275(1): 35 - 40. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X Yu, S Miyamoto, and E Mekada Integrin alpha 2 beta 1-dependent EGF receptor activation at cell-cell contact sites J. Cell Sci., January 6, 2000; 113(12): 2139 - 2147. [Abstract] [PDF]
|
|
|
|
|
|
S. Santoso, J. Amrhein, H. A. Hofmann, U. J.H. Sachs, M. M. Walka, H. Kroll, and V. Kiefel A Point Mutation Thr799Met on the alpha 2 Integrin Leads to the Formation of New Human Platelet Alloantigen Sita and Affects Collagen-Induced Aggregation Blood, December 15, 1999; 94(12): 4103 - 4111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Estavillo, A. Ritchie, T. G. Diacovo, and M. A. Cruz Functional Analysis of a Recombinant Glycoprotein Ia/IIa (Integrin alpha 2beta 1) I Domain That Inhibits Platelet Adhesion to Collagen and Endothelial Matrix under Flow Conditions J. Biol. Chem., December 10, 1999; 274(50): 35921 - 35926. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kamata, R. C. Liddington, and Y. Takada Interaction between Collagen and the alpha 2 I-domain of Integrin alpha 2beta 1. CRITICAL ROLE OF CONSERVED RESIDUES IN THE METAL ION-DEPENDENT ADHESION SITE (MIDAS) REGION J. Biol. Chem., November 5, 1999; 274(45): 32108 - 32111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Velling, M. Kusche-Gullberg, T. Sejersen, and D. Gullberg cDNA Cloning and Chromosomal Localization of Human alpha 11 Integrin. A COLLAGEN-BINDING, I DOMAIN-CONTAINING, beta 1-ASSOCIATED INTEGRIN alpha -CHAIN PRESENT IN MUSCLE TISSUES J. Biol. Chem., September 3, 1999; 274(36): 25735 - 25742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ivaska, J. Kapyla, O. Pentikainen, A.-M. Hoffren, J. Hermonen, P. Huttunen, M. S. Johnson, and J. Heino A Peptide Inhibiting the Collagen Binding Function of Integrin alpha 2I Domain J. Biol. Chem., February 5, 1999; 274(6): 3513 - 3521. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.G. Knight, L. F Morton, D. J Onley, A. R Peachey, T. Ichinohe, M. Okuma, R. W Farndale, and M. J Barnes Collagen-platelet interaction: Gly-Pro-Hyp is uniquely specific for platelet Gp VI and mediates platelet activation by collagen Cardiovasc Res, February 1, 1999; 41(2): 450 - 457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Li, P. Rieu, D. L. Griffith, D. Scott, and M. Amin Arnaout Two Functional States of the CD11b A-Domain: Correlations with Key Features of Two Mn2+-complexed Crystal Structures J. Cell Biol., December 14, 1998; 143(6): 1523 - 1534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. G. Knight, L. F. Morton, D. J. Onley, A. R. Peachey, A. J. Messent, P. A. Smethurst, D. S. Tuckwell, R. W. Farndale, and M. J. Barnes Identification in Collagen Type I of an Integrin alpha 2beta 1-binding Site Containing an Essential GER Sequence J. Biol. Chem., December 11, 1998; 273(50): 33287 - 33294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. McDowall, B. Leitinger, P. Stanley, P. A. Bates, A. M. Randi, and N. Hogg The I Domain of Integrin Leukocyte Function-associated Antigen-1 Is Involved in a Conformational Change Leading to High Affinity Binding to Ligand Intercellular Adhesion Molecule 1 (ICAM-1) J. Biol. Chem., October 16, 1998; 273(42): 27396 - 27403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. W. Griggs, C. M. Schmidt, and C. P. Carron Characteristics of Cation Binding to the I Domains of LFA-1 and MAC-1. THE LFA-1 I DOMAIN CONTAINS A Ca2+-BINDING SITE J. Biol. Chem., August 21, 1998; 273(34): 22113 - 22119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Newham, S. E. Craig, K. Clark, A. P. Mould, and M. J. Humphries Analysis of Ligand-Induced and Ligand-Attenuated Epitopes on the Leukocyte Integrin {alpha}4{beta}1: VCAM-1, Mucosal Addressin Cell Adhesion Molecule-1, and Fibronectin Induce Distinct Conformational Changes J. Immunol., May 1, 1998; 160(9): 4508 - 4517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Stanley and N. Hogg The I Domain of Integrin LFA-1 Interacts with ICAM-1 Domain 1 at Residue Glu-34 but Not Gln-73 J. Biol. Chem., February 6, 1998; 273(6): 3358 - 3362. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Messent, D. Tuckwell, V Knauper, M. Humphries, G Murphy, and J Gavrilovic Effects of collagenase-cleavage of type I collagen on alpha2beta1 integrin-mediated cell adhesion J. Cell Sci., January 4, 1998; 111(8): 1127 - 1135. [Abstract] [PDF]
|
|
|
|
|
|
J. Emsley, S. L. King, J. M. Bergelson, and R. C. Liddington Crystal Structure of the I Domain from Integrin alpha 2beta 1 J. Biol. Chem., November 7, 1997; 272(45): 28512 - 28517. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. King, T. Kamata, J. A. Cunningham, J. Emsley, R. C. Liddington, Y. Takada, and J. M. Bergelson Echovirus 1 Interaction with the Human Very Late Antigen-2 (Integrin alpha 2beta 1) I Domain. IDENTIFICATION OF TWO INDEPENDENT VIRUS CONTACT SITES DISTINCT FROM THE METAL ION-DEPENDENT ADHESION SITE J. Biol. Chem., November 7, 1997; 272(45): 28518 - 28522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bienkowska, M. Cruz, A. Atiemo, R. Handin, and R. Liddington The von Willebrand Factor A3 Domain Does Not Contain a Metal Ion-dependent Adhesion Site Motif J. Biol. Chem., October 3, 1997; 272(40): 25162 - 25167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-c. Ho, C. Heinemann, D. Hangan, S. Uniyal, V. L. Morris, and B. M.C. Chan Modulation of In Vivo Migratory Function of alpha 2beta 1 Integrin in Mouse Liver Mol. Biol. Cell, October 1, 1997; 8(10): 1863 - 1875. [Abstract] [Full Text]
|
|
|
|
|
|
S. K. Dickeson, J. J. Walsh, and S. A. Santoro Contributions of the I and EF Hand Domains to the Divalent Cation-dependent Collagen Binding Activity of the alpha 2beta 1 Integrin J. Biol. Chem., March 21, 1997; 272(12): 7661 - 7668. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. S. McKay, D. S. Annis, S. Honda, D. Christie, and T. J. Kunicki Molecular Requirements for Assembly and Function of a Minimized Human Integrin alpha IIbbeta 3 J. Biol. Chem., November 29, 1996; 271(48): 30544 - 30547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. C. Tozer, R. C. Liddington, M. J. Sutcliffe, A. H. Smeeton, and J. C. Loftus Ligand Binding to Integrin alpha IIbbeta 3 Is Dependent on a MIDAS-like Domain in the beta 3 Subunit J. Biol. Chem., September 6, 1996; 271(36): 21978 - 21984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Onley, C. G. Knight, D. S. Tuckwell, M. J. Barnes, and R. W. Farndale Micromolar Ca2+ Concentrations Are Essential for Mg2+-dependent Binding of Collagen by the Integrin alpha 2beta 1 in Human Platelets J. Biol. Chem., August 4, 2000; 275(32): 24560 - 24564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, S. Gurusiddappa, R. L. Rich, R. T. Owens, D. R. Keene, R. Mayne, A. Hook, and M. Hook Multiple Binding Sites in Collagen Type I for the Integrins alpha 1beta 1 and alpha 2beta 1 J. Biol. Chem., December 8, 2000; 275(50): 38981 - 38989. [Abstract] [Full Text] [PDF]
|
|
