|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 108, Issue 6 2487-2498, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
R Schwimmer and GK Ojakian
Department of Anatomy and Cell Biology, State University of New York Health Science Center, Brooklyn 11203, USA.
Previous studies have demonstrated that incubation of MDCK cell epithelial cysts in collagen gel induced a reversal in cell surface polarity that was regulated by beta 1 integrins. Further experiments were done to identify the specific collagen binding integrin involved by applying collagen gel overlays to the apical membrane of subconfluent MDCK monolayers. Cell surface levels of the apical membrane glycoprotein gp135 were monitored by ELISA to quantitate the extent of collagen-mediated membrane remodeling. After an 8 hour incubation with collagen, there was a 35% reduction in gp135 while the cell surface levels of the alpha 2, alpha 3 and beta 1 integrin subunits were not affected. Immunofluorescence microscopy confirmed the loss of gp135 from selected regions of the apical cell surface while the alpha 2 and beta 1 integrin subunits were distributed in small clusters over the entire apical membrane in both control and collagen-treated monolayers. Collagen-mediated loss of gp135 was inhibited by monoclonal antibodies which recognize either the alpha 2 or beta 1 integrin subunits but not by a monoclonal antibody against the alpha 6 beta 1 integrin. These results demonstrated that remodeling of the apical membrane had occurred, allowing the selective retention of beta 1 integrins but not gp135. They were supported by the observation that collagen-mediated loss of apical membrane microvilli was inhibited by the monoclonal antibody against the alpha 2 integrin subunit. Incubation of confluent monolayers with collagen gel induced the formation of polarized epithelial tubules within 16 hours. Epithelial tubule biogenesis was completely inhibited by monoclonal antibodies against either the alpha 2 or beta 1 integrin subunits, providing strong evidence that the alpha 2 beta 1 integrin is essential for collagen-mediated epithelial membrane remodeling and tubule formation.
This article has been cited by other articles:
|
|
 |
|
  T. Yamanaka, Y. Horikoshi, N. Izumi, A. Suzuki, K. Mizuno, and S. Ohno Lgl mediates apical domain disassembly by suppressing the PAR-3-aPKC-PAR-6 complex to orient apical membrane polarity J. Cell Sci., May 15, 2006; 119(10): 2107 - 2118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Eisen, S. Walid, D. R. Ratcliffe, and G. K. Ojakian Regulation of epithelial tubule formation by Rho family GTPases Am J Physiol Cell Physiol, May 1, 2006; 290(5): C1297 - C1309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Yu, A. Datta, P. Leroy, L. E. O'Brien, G. Mak, T.-S. Jou, K. S. Matlin, K. E. Mostov, and M. M.P. Zegers {beta}1-Integrin Orients Epithelial Polarity via Rac1 and Laminin Mol. Biol. Cell, February 1, 2005; 16(2): 433 - 445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Eisen, D. R. Ratcliffe, and G. K. Ojakian Modulation of epithelial tubule formation by Rho kinase Am J Physiol Cell Physiol, April 1, 2004; 286(4): C857 - C866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-K. Wang, H.-H. Lin, and M.-J. Tang Collagen gel overlay induces two phases of apoptosis in MDCK cells Am J Physiol Cell Physiol, June 1, 2001; 280(6): C1440 - C1448. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ojakian, D. Ratcliffe, and R Schwimmer Integrin regulation of cell-cell adhesion during epithelial tubule formation J. Cell Sci., January 3, 2001; 114(5): 941 - 952. [Abstract] [PDF]
|
|
|
|
 |
|
 C. YEAMAN, K. K. GRINDSTAFF, and W. J. NELSON New Perspectives on Mechanisms Involved in Generating Epithelial Cell Polarity Physiol Rev, January 1, 1999; 79(1): 73 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Xiao, D. Wang, M. D. Benson, G. Karsenty, and R. T. Franceschi Role of the alpha 2-Integrin in Osteoblast-specific Gene Expression and Activation of the Osf2 Transcription Factor J. Biol. Chem., December 4, 1998; 273(49): 32988 - 32994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gut, M. S. Balda, and K. Matter The Cytoplasmic Domains of a beta 1 Integrin Mediate Polarization in Madin-Darby Canine Kidney Cells by Selective Basolateral Stabilization J. Biol. Chem., November 6, 1998; 273(45): 29381 - 29388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-J. Tang, J.-J. Hu, H.-H. Lin, W.-T. Chiu, and S.-T. Jiang Collagen gel overlay induces apoptosis of polarized cells in cultures: disoriented cell death Am J Physiol Cell Physiol, October 1, 1998; 275(4): C921 - C931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R Herrera Modulation of hepatocyte growth factor-induced scattering of HT29 colon carcinoma cells. Involvement of the MAPK pathway J. Cell Sci., January 4, 1998; 111(8): 1039 - 1049. [Abstract] [PDF]
|
|
|
|
|
|
G. Ojakian, W. Nelson, and K. Beck Mechanisms for de novo biogenesis of an apical membrane compartment in groups of simple epithelial cells surrounded by extracellular matrix J. Cell Sci., January 11, 1997; 110(22): 2781 - 2794. [Abstract] [PDF]
|
|
|
|
|
|
A Zuk and K. Matlin Apical beta 1 integrin in polarized MDCK cells mediates tubulocyst formation in response to type I collagen overlay J. Cell Sci., January 7, 1996; 109(7): 1875 - 1889. [Abstract] [PDF]
|
|
