Journal of Cell Science, Vol 108, Issue 7 2663-2672, Copyright © 1995 by Company of Biologists

JOURNAL ARTICLES

Perlecan is a component of cartilage matrix and promotes chondrocyte attachment

N SundarRaj, D Fite, S Ledbetter, S Chakravarti and JR Hassell
Department of Ophthalmology, University of Pittsburgh School of Medicine, PA 15213, USA.

Aggrecan, a chondroitin/keratan sulfate-containing proteoglycan, is a major component of cartilaginous tissues. Immunolocalization studies, using antibodies directed to perlecan, a heparan sulfate proteoglycan first detected in basement membranes, and laminin (another major component of basement membranes), indicate that perlecan and laminin are also present in the matrices of hyaline cartilage in the nasal septum, the articular surface of the bone and the growth plate of the developing bone. Consequently, we used antibodies to both aggrecan and perlecan to characterize their synthesis and secretion by primary cultures of chondrocytes derived from the rat chondrosarcoma. Chondrocytes were pulsed for 20 minutes with [35S]methionine and then chased for up to six hours. The radiolabeled perlecan and aggrecan were immunoprecipitated and analyzed by SDS-PAGE. The results show that chondrocytes synthesize precursor proteins to both proteoglycans, but that only the aggrecan precursor protein is secreted as a proteoglycan. Perlecan was also secreted but with less posttranslational modifications than aggrecan. Northern blot analyses of the RNAs from immortalized rat chondrocytes indicated that the major mRNA encoding for perlecan was approximately 13 kb in length, similar in size to that expressed by other cell types, which synthesize 400 kDa core protein perlecan. Analyses of the proteoglycan fractions from the extracts of bovine articular surface indicated that perlecan in this tissue contains both chondroitin and heparan sulfate side-chains. Purified perlecan and laminin were found to promote attachment of immortalized rat chondrocytes in vitro. These studies indicated that perlecan, once thought to be a unique component of the basement membranes, is more widely distributed and is an important component of the cartilage matrix, where it may provide for cell adhesion to the matrix.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				K. D. Rodgers, T. Sasaki, A. Aszodi, and O. Jacenko Reduced perlecan in mice results in chondrodysplasia resembling Schwartz-Jampel syndrome Hum. Mol. Genet., March 1, 2007; 16(5): 515 - 528. [Abstract] [Full Text] [PDF]

				J. Melrose, P. Roughley, S. Knox, S. Smith, M. Lord, and J. Whitelock The Structure, Location, and Function of Perlecan, a Prominent Pericellular Proteoglycan of Fetal, Postnatal, and Mature Hyaline Cartilages J. Biol. Chem., December 1, 2006; 281(48): 36905 - 36914. [Abstract] [Full Text] [PDF]

				A. J. Kvist, A. E. Johnson, M. Morgelin, E. Gustafsson, E. Bengtsson, K. Lindblom, A. Aszodi, R. Fassler, T. Sasaki, R. Timpl, et al. Chondroitin Sulfate Perlecan Enhances Collagen Fibril Formation: IMPLICATIONS FOR PERLECAN CHONDRODYSPLASIAS J. Biol. Chem., November 3, 2006; 281(44): 33127 - 33139. [Abstract] [Full Text] [PDF]

				J. M. Allen, J. F. Bateman, U. Hansen, R. Wilson, P. Bruckner, R. T. Owens, T. Sasaki, R. Timpl, and J. Fitzgerald WARP Is a Novel Multimeric Component of the Chondrocyte Pericellular Matrix That Interacts with Perlecan J. Biol. Chem., March 17, 2006; 281(11): 7341 - 7349. [Abstract] [Full Text] [PDF]

				A.A. DeCarlo and J.M. Whitelock The Role of Heparan Sulfate and Perlecan in Bone-regenerative Procedures Journal of Dental Research, February 1, 2006; 85(2): 122 - 132. [Abstract] [Full Text] [PDF]

				J. H. D. Bassett, R. Swinhoe, O. Chassande, J. Samarut, and G. R. Williams Thyroid Hormone Regulates Heparan Sulfate Proteoglycan Expression in the Growth Plate Endocrinology, January 1, 2006; 147(1): 295 - 305. [Abstract] [Full Text] [PDF]

				J. Melrose, S. Smith, and J. Whitelock Perlecan Immunolocalizes to Perichondrial Vessels and Canals in Human Fetal Cartilaginous Primordia in Early Vascular and Matrix Remodeling Events Associated with Diarthrodial Joint Development J. Histochem. Cytochem., November 1, 2004; 52(11): 1405 - 1413. [Abstract] [Full Text] [PDF]

				T. Pihlajamaa, H. Lankinen, J. Ylostalo, L. Valmu, J. Jaalinoja, F. Zaucke, L. Spitznagel, S. Gosling, A. Puustinen, M. Morgelin, et al. Characterization of Recombinant Amino-terminal NC4 Domain of Human Collagen IX: INTERACTION WITH GLYCOSAMINOGLYCANS AND CARTILAGE OLIGOMERIC MATRIX PROTEIN J. Biol. Chem., June 4, 2004; 279(23): 24265 - 24273. [Abstract] [Full Text] [PDF]

				X. Jiang and J. R. Couchman Perlecan and Tumor Angiogenesis J. Histochem. Cytochem., November 1, 2003; 51(11): 1393 - 1410. [Abstract] [Full Text] [PDF]

				J. Melrose, S. Smith, P. Ghosh, and J. Whitelock Perlecan, the Multidomain Heparan Sulfate Proteoglycan of Basement Membranes, Is also a Prominent Component of the Cartilaginous Primordia in the Developing Human Fetal Spine J. Histochem. Cytochem., October 1, 2003; 51(10): 1331 - 1341. [Abstract] [Full Text] [PDF]

				M. Mongiat, S. M. Sweeney, J. D. San Antonio, J. Fu, and R. V. Iozzo Endorepellin, a Novel Inhibitor of Angiogenesis Derived from the C Terminus of Perlecan J. Biol. Chem., January 31, 2003; 278(6): 4238 - 4249. [Abstract] [Full Text] [PDF]

				M. Costell, R. Carmona, E. Gustafsson, M. Gonzalez-Iriarte, R. Fassler, and R. Munoz-Chapuli Hyperplastic Conotruncal Endocardial Cushions and Transposition of Great Arteries in Perlecan-Null Mice Circ. Res., July 26, 2002; 91(2): 158 - 164. [Abstract] [Full Text] [PDF]

				T. Vincent, M. Hermansson, M. Bolton, R. Wait, and J. Saklatvala Basic FGF mediates an immediate response of articular cartilage to mechanical injury PNAS, June 11, 2002; 99(12): 8259 - 8264. [Abstract] [Full Text] [PDF]

				P. Govindraj, L. West, T. J. Koob, P. Neame, K. Doege, and J. R. Hassell Isolation and Identification of the Major Heparan Sulfate Proteoglycans in the Developing Bovine Rib Growth Plate J. Biol. Chem., May 24, 2002; 277(22): 19461 - 19469. [Abstract] [Full Text] [PDF]

				E. Bengtsson, M. Morgelin, T. Sasaki, R. Timpl, D. Heinegard, and A. Aspberg The Leucine-rich Repeat Protein PRELP Binds Perlecan and Collagens and May Function as a Basement Membrane Anchor J. Biol. Chem., April 19, 2002; 277(17): 15061 - 15068. [Abstract] [Full Text] [PDF]

				N. R. Bhakta, A. M. Garcia, E. H. Frank, A. J. Grodzinsky, and T. I. Morales The Insulin-like Growth Factors (IGFs) I and II Bind to Articular Cartilage via the IGF-binding Proteins J. Biol. Chem., February 25, 2000; 275(8): 5860 - 5866. [Abstract] [Full Text] [PDF]

				A. J. A. Groffen, J. H. Veerkamp, L. A. H. Monnens, and L. P. W. J. van den Heuvel Recent insights into the structure and functions of heparan sulfate proteoglycans in the human glomerular basement membrane Nephrol. Dial. Transplant., September 1, 1999; 14(9): 2119 - 2129. [Abstract] [Full Text] [PDF]

				M.M. French, S.E. Smith, K. Akanbi, T. Sanford, J. Hecht, M.C. Farach-Carson, and D.D. Carson Expression of the Heparan Sulfate Proteoglycan, Perlecan, during Mouse Embryogenesis and Perlecan Chondrogenic Activity In Vitro J. Cell Biol., May 31, 1999; 145(5): 1103 - 1115. [Abstract] [Full Text] [PDF]

				W. B. Valhmu, G. D. Palmer, J. Dobson, S. G. Fischer, and A. Ratcliffe Regulatory Activities of the 5'- and 3'-Untranslated Regions and Promoter of the Human Aggrecan Gene J. Biol. Chem., March 13, 1998; 273(11): 6196 - 6202. [Abstract] [Full Text] [PDF]

				J. R. Couchman, R. Kapoor, M. Sthanam, and R.-R. Wu Perlecan and Basement Membrane-Chondroitin Sulfate Proteoglycan (Bamacan) Are Two Basement Membrane Chondroitin/Dermatan Sulfate Proteoglycans in the Engelbreth-Holm-Swarm Tumor Matrix J. Biol. Chem., April 19, 1996; 271(16): 9595 - 9602. [Abstract] [Full Text] [PDF]

				M. Mongiat, J. Otto, R. Oldershaw, F. Ferrer, J. D. Sato, and R. V. Iozzo Fibroblast Growth Factor-binding Protein Is a Novel Partner for Perlecan Protein Core J. Biol. Chem., March 23, 2001; 276(13): 10263 - 10271. [Abstract] [Full Text] [PDF]