|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 108, Issue 8 2781-2790, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
E Barbarese, DE Koppel, MP Deutscher, CL Smith, K Ainger, F Morgan and JH Carson
Department of Neurology, University of Connecticut Health Center, Farmington 06030, USA.
The intracellular distribution of various components of the protein translational machinery was visualized in mouse oligodendrocytes in culture using high resolution fluorescence in situ hybridization and immunofluorescence in conjunction with dual channel confocal laser scanning microscopy. Arginyl-tRNA synthetase, elongation factor 1a, ribosomal RNA, and myelin basic protein mRNA were all co-localized in granules in the processes, veins and membrane sheets of the cell. Colocalization was evaluated by dual channel cross correlation analysis to determine the correlation index (% colocalization) and correlation distance (granule radius), and by single granule ratiometric analysis to determine the distribution of the different components in individual granules. Most granules contained synthetase, elongation factor, ribosomal RNA and myelin basic protein mRNA. These results indicate that several different components of the protein synthetic machinery, including aminoacyl-tRNA synthetases, elongation factors, ribosomes and mRNAs, are colocalized in granules in oligodendrocytes. We propose that these granules are supramolecular complexes containing all of the necessary macromolecular components for protein translation and that they represent a heretofore undescribed subcellular organization of the protein synthetic machinery. This spatial organization may increase the efficiency of protein synthesis and may also provide a vehicle for transport and localization of specific mRNAs within the cell.
This article has been cited by other articles:
|
|
 |
|
  C. S. Raju, C. Goritz, Y. Nord, O. Hermanson, C. Lopez-Iglesias, N. Visa, G. Castelo-Branco, and P. Percipalle In Cultured Oligodendrocytes the A/B-type hnRNP CBF-A Accompanies MBP mRNA Bound to mRNA Trafficking Sequences Mol. Biol. Cell, July 1, 2008; 19(7): 3008 - 3019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Gao, V. Tatavarty, G. Korza, M. K. Levin, and J. H. Carson Multiplexed Dendritic Targeting of {alpha} Calcium Calmodulin-dependent Protein Kinase II, Neurogranin, and Activity-regulated Cytoskeleton-associated Protein RNAs by the A2 Pathway Mol. Biol. Cell, May 1, 2008; 19(5): 2311 - 2327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. B. Werner, K. Kuhlmann, S. Shen, M. Uecker, A. Schardt, K. Dimova, F. Orfaniotou, A. Dhaunchak, B. G. Brinkmann, W. Mobius, et al. Proteolipid Protein Is Required for Transport of Sirtuin 2 into CNS Myelin J. Neurosci., July 18, 2007; 27(29): 7717 - 7730. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. D. Comeau, S. Costantino, and P. W. Wiseman A Guide to Accurate Fluorescence Microscopy Colocalization Measurements Biophys. J., December 15, 2006; 91(12): 4611 - 4622. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-L. Ong, P.-C. Lin, X. Zhang, S.-M. Chia, and H. Yu Kinectin-dependent Assembly of Translation Elongation Factor-1 Complex on Endoplasmic Reticulum Regulates Protein Synthesis J. Biol. Chem., November 3, 2006; 281(44): 33621 - 33634. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. D. Kosturko, M. J. Maggipinto, G. Korza, J. W. Lee, J. H. Carson, and E. Barbarese Heterogeneous Nuclear Ribonucleoprotein (hnRNP) E1 Binds to hnRNP A2 and Inhibits Translation of A2 Response Element mRNAs Mol. Biol. Cell, August 1, 2006; 17(8): 3521 - 3533. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Scholz, P. McDermott, M. Garnovskaya, T. N. Gallien, S. Huettelmaier, C. DeRienzo, and G. Cooper IV Microtubule-associated protein-4 (MAP-4) inhibits microtubule-dependent distribution of mRNA in isolated neonatal cardiocytes Cardiovasc Res, August 1, 2006; 71(3): 506 - 516. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Small-Howard, N. Morozova, Z. Stoytcheva, E. P. Forry, J. B. Mansell, J. W. Harney, B. A. Carlson, X.-m. Xu, D. L. Hatfield, and M. J. Berry Supramolecular complexes mediate selenocysteine incorporation in vivo. Mol. Cell. Biol., March 1, 2006; 26(6): 2337 - 2346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Anitei, M. Ifrim, M.-A. Ewart, A. E. Cowan, J. H. Carson, R. Bansal, and S. E. Pfeiffer A role for Sec8 in oligodendrocyte morphological differentiation J. Cell Sci., March 1, 2006; 119(5): 807 - 818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Tretyakova, A. S. Zolotukhin, W. Tan, J. Bear, F. Propst, G. Ruthel, and B. K. Felber Nuclear Export Factor Family Protein Participates in Cytoplasmic mRNA Trafficking J. Biol. Chem., September 9, 2005; 280(36): 31981 - 31990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Mingle, N. N. Okuhama, J. Shi, R. H. Singer, J. Condeelis, and G. Liu Localization of all seven messenger RNAs for the actin-polymerization nucleator Arp2/3 complex in the protrusions of fibroblasts J. Cell Sci., June 1, 2005; 118(11): 2425 - 2433. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. RODRIGUEZ, S. A. SEIPEL, D. R. HAMILL, D. P. ROMANCINO, M. DI CARLO, K. A. SUPRENANT, and E. M. BONDER Seawi--a sea urchin piwi/argonaute family member is a component of MT-RNP complexes RNA, May 1, 2005; 11(5): 646 - 656. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Kavanagh, T. C. Schulz, P. Davey, C. Claudianos, C. Russell, and P. D. Rathjen A family of RS domain proteins with novel subcellular localization and trafficking Nucleic Acids Res., March 1, 2005; 33(4): 1309 - 1322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Thomas, L. J. M. Tosar, M. Loschi, J. M. Pasquini, J. Correale, S. Kindler, and G. L. Boccaccio Staufen Recruitment into Stress Granules Does Not Affect Early mRNA Transport in Oligodendrocytes Mol. Biol. Cell, January 1, 2005; 16(1): 405 - 420. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Smith Moving Molecules: mRNA Trafficking in Mammalian Oligodendrocytes and Neurons Neuroscientist, December 1, 2004; 10(6): 495 - 500. [Abstract] [PDF]
|
|
|
|
|
|
H. Wang and H. Tiedge Translational Control at the Synapse Neuroscientist, October 1, 2004; 10(5): 456 - 466. [Abstract] [PDF]
|
|
|
|
|
|
S. W. Lee, B. H. Cho, S. G. Park, and S. Kim Aminoacyl-tRNA synthetase complexes: beyond translation J. Cell Sci., September 1, 2004; 117(17): 3725 - 3734. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. V. Costes, D. Daelemans, E. H. Cho, Z. Dobbin, G. Pavlakis, and S. Lockett Automatic and Quantitative Measurement of Protein-Protein Colocalization in Live Cells Biophys. J., June 1, 2004; 86(6): 3993 - 4003. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Kress, Y. J. Yoon, and K. L. Mowry Nuclear RNP complex assembly initiates cytoplasmic RNA localization J. Cell Biol., April 26, 2004; 165(2): 203 - 211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-K. Lee and P. J. Hollenbeck Organization and translation of mRNA in sympathetic axons J. Cell Sci., November 1, 2003; 116(21): 4467 - 4478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shan, T. P. Munro, E. Barbarese, J. H. Carson, and R. Smith A Molecular Mechanism for mRNA Trafficking in Neuronal Dendrites J. Neurosci., October 1, 2003; 23(26): 8859 - 8866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Farina, S. Huttelmaier, K. Musunuru, R. Darnell, and R. H. Singer Two ZBP1 KH domains facilitate {beta}-actin mRNA localization, granule formation, and cytoskeletal attachment J. Cell Biol., January 2, 2003; 160(1): 77 - 87. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. W. Ma, K. Moran-Jones, J. Shan, T. P. Munro, M. J. Snee, K. S. Hoek, and R. Smith Heterogeneous Nuclear Ribonucleoprotein A3, a Novel RNA Trafficking Response Element-binding Protein J. Biol. Chem., May 10, 2002; 277(20): 18010 - 18020. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Kosik and A. M. Krichevsky The Message and the Messenger: Delivering RNA in Neurons Sci. Signal., April 2, 2002; 2002 (126): pe16 - pe16. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Eystathioy, E. K. L. Chan, S. A. Tenenbaum, J. D. Keene, K. Griffith, and M. J. Fritzler A Phosphorylated Cytoplasmic Autoantigen, GW182, Associates with a Unique Population of Human mRNAs within Novel Cytoplasmic Speckles Mol. Biol. Cell, April 1, 2002; 13(4): 1338 - 1351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Aronov, G. Aranda, L. Behar, and I. Ginzburg Visualization of translated tau protein in the axons of neuronal P19 cells and characterization of tau RNP granules J. Cell Sci., January 10, 2002; 115(19): 3817 - 3827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mallardo, S. Schleich, and J. Krijnse Locker Microtubule-dependent Organization of Vaccinia Virus Core-derivd Early mRNAs into Distinct Cytoplasmic Structures Mol. Biol. Cell, December 1, 2001; 12(12): 3875 - 3891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Azad, D. R. Stanford, S. Sarkar, and A. K. Hopper Role of Nuclear Pools of Aminoacyl-tRNA Synthetases in tRNA Nuclear Export Mol. Biol. Cell, May 1, 2001; 12(5): 1381 - 1392. [Abstract] [Full Text]
|
|
|
|
|
|
A. J. Mouland, H. Xu, H. Cui, W. Krueger, T. P. Munro, M. Prasol, J. Mercier, D. Rekosh, R. Smith, E. Barbarese, et al. RNA Trafficking Signals in Human Immunodeficiency Virus Type 1 Mol. Cell. Biol., March 15, 2001; 21(6): 2133 - 2143. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Grosshans, E. Hurt, and G. Simos An aminoacylation-dependent nuclear tRNA export pathway in yeast Genes & Dev., April 1, 2000; 14(7): 830 - 840. [Abstract] [Full Text]
|
|
|
|
|
|
T. P. Munro, R. J. Magee, G. J. Kidd, J. H. Carson, E. Barbarese, L. M. Smith, and R. Smith Mutational Analysis of a Heterogeneous Nuclear Ribonucleoprotein A2 Response Element for RNA Trafficking J. Biol. Chem., November 26, 1999; 274(48): 34389 - 34395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.L. Zhang, R.H. Singer, and G.J. Bassell Neurotrophin Regulation of {beta}-Actin mRNA and Protein Localization within Growth Cones J. Cell Biol., October 4, 1999; 147(1): 59 - 70. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. E. Aranda-Abreu, L. Behar, S. Chung, H. Furneaux, and I. Ginzburg Embryonic Lethal Abnormal Vision-Like RNA-Binding Proteins Regulate Neurite Outgrowth and Tau Expression in PC12 Cells J. Neurosci., August 15, 1999; 19(16): 6907 - 6917. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. BASSELL, Y. OLEYNIKOV, and R. H. SINGER The travels of mRNAs through all cells large and small FASEB J, March 1, 1999; 13(3): 447 - 454. [Full Text]
|
|
|
|
|
|
R.-P. JANSEN RNA–cytoskeletal associations FASEB J, March 1, 1999; 13(3): 455 - 466. [Abstract] [Full Text]
|
|
|
|
|
|
B. S. Negrutskii, V. F. Shalak, P. Kerjan, A. V. El'skaya, and M. Mirande Functional Interaction of Mammalian Valyl-tRNA Synthetase with Elongation Factor EF-1alpha in the Complex with EF-1H J. Biol. Chem., February 19, 1999; 274(8): 4545 - 4550. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E.-L. Punnonen, C. Fages, J. Wartiovaara, and H. Rauvala Ultrastructural Localization of ß-Actin and Amphoterin mRNA in Cultured Cells: Application of Tyramide Signal Amplification and Comparison of Detection Methods J. Histochem. Cytochem., January 1, 1999; 47(1): 99 - 112. [Abstract] [Full Text]
|
|
|
|
|
|
L. Gangwani, M. Mikrut, Z. Galcheva-Gargova, and R. J. Davis Interaction of ZPR1 with Translation Elongation Factor-1alpha in Proliferating Cells J. Cell Biol., December 14, 1998; 143(6): 1471 - 1484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R Martin, B Vaida, R Bleher, M Crispino, and A Giuditta Protein synthesizing units in presynaptic and postsynaptic domains of squid neurons J. Cell Sci., January 11, 1998; 111(21): 3157 - 3166. [Abstract] [PDF]
|
|
|
|
|
|
F. Iborra, D. Jackson, and P. Cook The path of transcripts from extra-nucleolar synthetic sites to nuclear pores: transcripts in transit are concentrated in discrete structures containing SR proteins J. Cell Sci., January 8, 1998; 111(15): 2269 - 2282. [Abstract] [PDF]
|
|
|
|
|
|
D Antic and J. Keene Messenger ribonucleoprotein complexes containing human ELAV proteins: interactions with cytoskeleton and translational apparatus J. Cell Sci., January 1, 1998; 111(2): 183 - 197. [Abstract] [PDF]
|
|
|
|
 |
|
 R. B. Knowles and K. S. Kosik Neurotrophin-3 signals redistribute RNA in neurons PNAS, December 23, 1997; 94(26): 14804 - 14808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Stapulionis, S. Kolli, and M. P. Deutscher Efficient Mammalian Protein Synthesis Requires an Intact F-Actin System J. Biol. Chem., October 3, 1997; 272(40): 24980 - 24986. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ainger, D. Avossa, A. S. Diana, C. Barry, E. Barbarese, and J. H. Carson Transport and Localization Elements in Myelin Basic Protein mRNA J. Cell Biol., September 8, 1997; 138(5): 1077 - 1087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Racca, A. Gardiol, and A. Triller Dendritic and Postsynaptic Localizations of Glycine Receptor alpha Subunit mRNAs J. Neurosci., March 1, 1997; 17(5): 1691 - 1700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. B. Knowles, J. H. Sabry, M. E. Martone, T. J. Deerinck, M. H. Ellisman, G. J. Bassell, and K. S. Kosik Translocation of RNA Granules in Living Neurons J. Neurosci., December 15, 1996; 16(24): 7812 - 7820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. King, E. Barbarese, J. F. Dillman III, R. S. Patel-King, J. H. Carson, and K. K. Pfister Brain Cytoplasmic and Flagellar Outer Arm Dyneins Share a Highly Conserved Mr 8,000Light Chain J. Biol. Chem., August 9, 1996; 271(32): 19358 - 19366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Nathanson and M. P. Deutscher Active Aminoacyl-tRNA Synthetases Are Present in Nuclei as a High Molecular Weight Multienzyme Complex J. Biol. Chem., October 6, 2000; 275(41): 31559 - 31562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Warner, M. J. Berry, M. E. Moustafa, B. A. Carlson, D. L. Hatfield, and J. R. Faust Inhibition of Selenoprotein Synthesis by Selenocysteine tRNA[Ser]Sec Lacking Isopentenyladenosine J. Biol. Chem., September 1, 2000; 275(36): 28110 - 28119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. X. Chuong, R. T. Mullen, and D. G. Muench Identification of a Rice RNA- and Microtubule-binding Protein as the Multifunctional Protein, a Peroxisomal Enzyme Involved in the beta -Oxidation of Fatty Acids J. Biol. Chem., January 18, 2002; 277(4): 2419 - 2429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Liu, W. M. Grant, D. Persky, V. M. Latham Jr., R. H. Singer, and J. Condeelis Interactions of Elongation Factor 1alpha with F-Actin and beta -Actin mRNA: Implications for Anchoring mRNA in Cell Protrusions Mol. Biol. Cell, February 1, 2002; 13(2): 579 - 592. [Abstract] [Full Text] [PDF]
|
|
