|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 108, Issue 9 3067-3078, Copyright © 1995 by Company of Biologists
JOURNAL ARTICLES |
A Cattelino, R Longhi and I de Curtis
Department of Biological and Technological Research (DIBIT), S. Raffaele Scientific Institute, Milano, Italy.
The integrin alpha 6 beta 1 is a receptor involved in the adhesion of several cell types to laminin. By using function-blocking antibodies, we have shown that alpha 6 beta 1 is a functional laminin receptor in chick embryo fibroblasts. We also found that these cells express two variants of the alpha 6 subunit, alpha 6A and alpha 6B, characterized by different cytoplasmic domains. By using indirect immunofluorescence with isoform-specific polyclonal antibodies, we showed that the two isoforms of the alpha 6 subunit distribute differently on the ventral plasma membrane of these cells cultured on laminin-coated substrates. In fact, while the alpha 6A subunit was found codistributing with vinculin in focal contacts, the alpha 6B subunit showed a homogeneously distributed punctate pattern. This difference was particularly evident when preparations of ventral plasma membranes were used for the immunolocalization. Furthermore, when cells were cultured on fibronectin, a substrate not recognized by the alpha 6 beta 1 laminin receptor, the distribution of the two alpha 6 isoforms was similar to that observed on laminin, with alpha 6A still colocalizing with vinculin in focal adhesions. Our results indicate that two forms of the alpha 6 beta 1 laminin receptor coexpressed in the same cells show distinctive distributions, and suggest that receptor occupancy by laminin is not essential for the accumulation of the alpha 6A beta 1 integrin in adhesion plaques.
This article has been cited by other articles:
|
|
 |
|
  A. Totaro, S. Paris, C. Asperti, and I. de Curtis Identification of an Intramolecular Interaction Important for the Regulation of GIT1 Functions Mol. Biol. Cell, December 1, 2007; 18(12): 5124 - 5138. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Bouvard, L. Vignoud, S. Dupe-Manet, N. Abed, H.-N. Fournier, C. Vincent-Monegat, S. F. Retta, R. Fassler, and M. R. Block Disruption of Focal Adhesions by Integrin Cytoplasmic Domain-associated Protein-1alpha J. Biol. Chem., February 14, 2003; 278(8): 6567 - 6574. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Matafora, S. Paris, S. Dariozzi, and I. d. Curtis Molecular mechanisms regulating the subcellular localization of p95-APP1 between the endosomal recycling compartment and sites of actin organization at the cell surface J. Cell Sci., March 14, 2002; 114(24): 4509 - 4520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cattelino, C. Albertinazzi, M. Bossi, D. R. Critchley, and I. de Curtis A Cell-free System to Study Regulation of Focal Adhesions and of the Connected Actin Cytoskeleton Mol. Biol. Cell, February 1, 1999; 10(2): 373 - 391. [Abstract] [Full Text]
|
|
|
|
|
|
C Albertinazzi, A Cattelino, and I de Curtis Rac GTPases localize at sites of actin reorganization during dynamic remodeling of the cytoskeleton of normal embryonic fibroblasts J. Cell Sci., January 11, 1999; 112(21): 3821 - 3831. [Abstract] [PDF]
|
|
|
|
 |
|
 C. Gimond, C. Baudoin, R. van der Neut, D. Kramer, J. Calafat, and A. Sonnenberg Cre-loxP-mediated Inactivation of the alpha 6A Integrin Splice Variant In Vivo: Evidence for a Specific Functional Role of alpha 6A in Lymphocyte Migration but Not in Heart Development J. Cell Biol., October 5, 1998; 143(1): 253 - 266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Albertinazzi, D. Gilardelli, S. Paris, R. Longhi, and I. de Curtis Overexpression of a Neural-specific Rho Family GTPase, cRac1B, Selectively Induces Enhanced Neuritogenesis and Neurite Branching in Primary Neurons J. Cell Biol., August 10, 1998; 142(3): 815 - 825. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Malosio, D. Gilardelli, S. Paris, C. Albertinazzi, and I. de Curtis Differential Expression of Distinct Members of Rho Family GTP-Binding Proteins during Neuronal Development: Identification of Rac1B, a New Neural-Specific Member of the Family J. Neurosci., September 1, 1997; 17(17): 6717 - 6728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N Desban and J. Duband Avian neural crest cell migration on laminin: interaction of the alpha1beta1 integrin with distinct laminin-1 domains mediates different adhesive responses J. Cell Sci., January 11, 1997; 110(21): 2729 - 2744. [Abstract] [PDF]
|
|
