|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 109, Issue 10 2443-2452, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
S de la Luna, MJ Burden, CW Lee and NB La Thangue
Division of Biochemistry and Molecular Biology, University of Glasgow, UK.
The cellular transcription factor E2F plays a critical role in integrating cell cycle progression with the transcription apparatus by virtue of a physical interaction and control by key regulators of the cell cycle, such as pRb, cyclins and cyclin-dependent kinases. Generic E2F DNA binding activity arises when a member of two families of proteins, E2F and DP, form heterodimeric complexes, an interaction which results in co-operative transcriptional and DNA binding activity. Here, we characterise a new and hitherto unexpected mechanism of control influencing the activity of E2F which is mediated at the level of intracellular location through a dependence on heterodimer formation for nuclear translocation. Nuclear accumulation is dramatically influenced by two distinct processes: alternative splicing of a nuclear localization signal and subunit composition of the E2F heterodimer. These data define a new level of control in the E2F transcription factor whereby interplay between subunits dictates the levels of nuclear DNA binding activity.
This article has been cited by other articles:
|
|
 |
|
  F. Zhou, L. Zhang, A. Wang, B. Song, K. Gong, L. Zhang, M. Hu, X. Zhang, N. Zhao, and Y. Gong The Association of GSK3{beta} with E2F1 Facilitates Nerve Growth Factor-induced Neural Cell Differentiation J. Biol. Chem., May 23, 2008; 283(21): 14506 - 14515. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. N. Garnovskaya, Y. V. Mukhin, T. M. Vlasova, J. S. Grewal, M. E. Ullian, B. G. Tholanikunnel, and J. R. Raymond Mitogen-induced Rapid Phosphorylation of Serine 795 of the Retinoblastoma Gene Product in Vascular Smooth Muscle Cells Involves ERK Activation J. Biol. Chem., June 4, 2004; 279(23): 24899 - 24905. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Scheijen, M. Bronk, T. van der Meer, D. De Jong, and R. Bernards High Incidence of Thymic Epithelial Tumors in E2F2 Transgenic Mice J. Biol. Chem., March 12, 2004; 279(11): 10476 - 10483. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ambrosino, C. Palmieri, A. Puca, F. Trimboli, M. Schiavone, F. Olimpico, M. R. Ruocco, F. di Leva, M. Toriello, I. Quinto, et al. Physical and Functional Interaction of HIV-1 Tat with E2F-4, a Transcriptional Regulator of Mammalian Cell Cycle J. Biol. Chem., August 23, 2002; 277(35): 31448 - 31458. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kosugi and Y. Ohashi E2Ls, E2F-like Repressors of Arabidopsis That Bind to E2F Sites in a Monomeric Form J. Biol. Chem., May 3, 2002; 277(19): 16553 - 16558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Mariconti, B. Pellegrini, R. Cantoni, R. Stevens, C. Bergounioux, R. Cella, and D. Albani The E2F Family of Transcription Factors from Arabidopsis thaliana. NOVEL AND CONSERVED COMPONENTS OF THE RETINOBLASTOMA/E2F PATHWAY IN PLANTS J. Biol. Chem., March 15, 2002; 277(12): 9911 - 9919. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Nili, G. S. Cojocaru, Y. Kalma, D. Ginsberg, N. G. Copeland, D. J. Gilbert, N. A. Jenkins, R. Berger, S. Shaklai, N. Amariglio, et al. Nuclear membrane protein LAP2{beta} mediates transcriptional repression alone and together with its binding partner GCL (germ-cell-less) J. Cell Sci., March 11, 2002; 114(18): 3297 - 3307. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Gill and P. A. Hamel Subcellular Compartmentalization of E2F Family Members Is Required for Maintenance of the Postmitotic State in Terminally Differentiated Muscle J. Cell Biol., March 20, 2000; 148(6): 1187 - 1202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Loughran and N. B. La Thangue Apoptotic and Growth-Promoting Activity of E2F Modulated by MDM2 Mol. Cell. Biol., March 15, 2000; 20(6): 2186 - 2197. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. Vogt, K. Zerfaß-Thome, A. Schulze, J. W. Botz, W. Zwerschke, and P. Jansen-Dürr Regulation of cyclin E gene expression by the human papillomavirus type 16 E7 oncoprotein J. Gen. Virol., August 1, 1999; 80(8): 2103 - 2113. [Abstract] [Full Text]
|
|
|
|
|
|
O. Stoss, F.-W. Schwaiger, T. A. Cooper, and S. Stamm Alternative Splicing Determines the Intracellular Localization of the Novel Nuclear Protein Nop30 and Its Interaction with the Splicing Factor SRp30c J. Biol. Chem., April 16, 1999; 274(16): 10951 - 10962. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Di Fiore, G. Guarguaglini, A. Palena, R. M. Kerkhoven, R. Bernards, and P. Lavia Two E2F Sites Control Growth-regulated and Cell Cycle-regulated Transcription of the Htf9-a/RanBP1 Gene through Functionally Distinct Mechanisms J. Biol. Chem., April 9, 1999; 274(15): 10339 - 10348. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Dyson The regulation of E2F by pRB-family proteins Genes & Dev., August 1, 1998; 12(15): 2245 - 2262. [Full Text]
|
|
|
|
 |
|
 A. Schulze, B. Mannhardt, K. Zerfass-Thome, W. Zwerschke, and P. Jansen-Durr Anchorage-Independent Transcription of the Cyclin A Gene Induced by the E7 Oncoprotein of Human Papillomavirus Type 16 J. Virol., March 1, 1998; 72(3): 2323 - 2334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Allen, S de la Luna, R. Kerkhoven, R Bernards, and N. La Thangue Distinct mechanisms of nuclear accumulation regulate the functional consequence of E2F transcription factors J. Cell Sci., January 11, 1997; 110(22): 2819 - 2831. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Kosugi and Y. Ohashi Interaction of the Arabidopsis E2F and DP Proteins Confers Their Concomitant Nuclear Translocation and Transactivation Plant Physiology, March 1, 2002; 128(3): 833 - 843. [Abstract] [Full Text] [PDF]
|
|
