|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 109, Issue 10 2509-2520, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
SE Baker, SB Hopkinson, M Fitchmun, GL Andreason, F Frasier, G Plopper, V Quaranta and JC Jones
Department of Cell and Molecular Biology, Northwestern University Medical School, Chicago, IL, USA.
Hemidesmosomes are complex macromolecular structures which integrate elements of the extracellular matrix and the cytoskeleton of epithelial cells. To characterize cell-matrix interactions in the hemidesmosome, we have made use of 804G cells which possess the unusual ability to assemble hemidesmosomes in vitro. During the course of our studies, we have raised a set of monoclonal antibodies against rat laminin-5, the major structural element comprising 804G matrix. One of these, termed CM6, recognizes the 150 kDa alpha chain of rat laminin-5 and binds the globular (G) domain of intact laminin-5 molecules as determined by rotary shadowing. CM6 antibodies perturb formed hemidesmosomes in 804G cells. In particular, within 1 hour of incubation of 804G cells with CM6 antibodies, colocalization of laminin-5 and alpha 6 beta 4 integrin is lost and by 2 hours, staining generated by hemidesmosomal antibodies appears primarily cytoplasmic in the perinuclear zone. Ultrastructurally, CM6 antibodies first appear to induce detachment of hemidesmosomes from the underlying matrix. Next, portions of the basal cell surface invaginate to form vesicles whose cytoplasmic-facing surface is coated with hemidesmosomes still associated with keratin intermediate filaments. Anchoring filaments extend into the inside compartment of the vesicles. We have also studied the impact of CM6 antibodies on a model system in which the matrix of 804G cells induces de novo assembly of hemidesmosomes in human keratinocytes. This process involves the plasma membrane reorganization of the hemidesmosome associated integrin alpha 6 beta 4 as well as a redistribution of other hemidesmosome components such as the 230 kDa bullous pemphigoid antigen. Pretreatment of 804G matrix with CM6 antibodies blocks such plasma membrane reorganization of hemidesmosome components and inhibits hemidesmosome formation. Our studies indicate a crucial role for the G domain of the alpha chain of laminin-5 in both nucleation of hemidesmosome assembly as well as maintenance of hemidesmosome structural integrity.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  C.-Y. Cheng, C.-C. Sun, W.-H. Yu, H.-L. Hsieh, D. H.-K. Ma, J.-H. S. Pang, and C.-M. Yang Novel Laminin 5 {gamma}2-chain Fragments Potentiating the Limbal Epithelial Cell Outgrowth on Amniotic Membrane Invest. Ophthalmol. Vis. Sci., October 1, 2009; 50(10): 4631 - 4639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Atkinson, H. M. Toennies, K. Holmbeck, and R. M. Senior Membrane type 1 matrix metalloproteinase is necessary for distal airway epithelial repair and keratinocyte growth factor receptor expression after acute injury Am J Physiol Lung Cell Mol Physiol, September 1, 2007; 293(3): L600 - L610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ogawa, Y. Tsubota, J. Hashimoto, Y. Kariya, and K. Miyazaki The Short Arm of Laminin {gamma}2 Chain of Laminin-5 (Laminin-332) Binds Syndecan-1 and Regulates Cellular Adhesion and Migration by Suppressing Phosphorylation of Integrin beta4 Chain Mol. Biol. Cell, May 1, 2007; 18(5): 1621 - 1633. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. DeBiase, K. Lane, S. Budinger, K. Ridge, M. Wilson, and J. C.R. Jones Laminin-311 (Laminin-6) Fiber Assembly by Type I-like Alveolar Cells J. Histochem. Cytochem., June 1, 2006; 54(6): 665 - 672. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Bystrom, I. Virtanen, P. Rousselle, D. Gullberg, and F. Pedrosa-Domellof Distribution of laminins in the developing human eye. Invest. Ophthalmol. Vis. Sci., March 1, 2006; 47(3): 777 - 785. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. T. Chartier, M. Laine, S. Gout, G. Pawlak, C. A. Marie, P. Matos, M. R. Block, and M. R. Jacquier-Sarlin Laminin-5-integrin interaction signals through PI 3-kinase and Rac1b to promote assembly of adherens junctions in HT-29 cells J. Cell Sci., January 1, 2006; 119(1): 31 - 46. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Nakashima, Y. Kariya, C. Yasuda, and K. Miyazaki Regulation of Cell Adhesion and Type VII Collagen Binding by the {beta}3 Chain Short Arm of Laminin-5: Effect of Its Proteolytic Cleavage J. Biochem., November 1, 2005; 138(5): 539 - 552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. R. Jones, K. Lane, S. B. Hopkinson, E. Lecuona, R. C. Geiger, D. A. Dean, E. Correa-Meyer, M. Gonzales, K. Campbell, J. I. Sznajder, et al. Laminin-6 assembles into multimolecular fibrillar complexes with perlecan and participates in mechanical-signal transduction via a dystroglycan-dependent, integrin-independent mechanism J. Cell Sci., June 15, 2005; 118(12): 2557 - 2566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tsubota, C. Yasuda, Y. Kariya, T. Ogawa, T. Hirosaki, H. Mizushima, and K. Miyazaki Regulation of Biological Activity and Matrix Assembly of Laminin-5 by COOH-terminal, LG4-5 Domain of {alpha}3 Chain J. Biol. Chem., April 15, 2005; 280(15): 14370 - 14377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Koshikawa, T. Minegishi, A. Sharabi, V. Quaranta, and M. Seiki Membrane-type Matrix Metalloproteinase-1 (MT1-MMP) Is a Processing Enzyme for Human Laminin {gamma}2 Chain J. Biol. Chem., January 7, 2005; 280(1): 88 - 93. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. R. Vives, E. Crublet, J.-P. Andrieu, J. Gagnon, P. Rousselle, and H. Lortat-Jacob A Novel Strategy for Defining Critical Amino Acid Residues Involved in Protein/Glycosaminoglycan Interactions J. Biol. Chem., December 24, 2004; 279(52): 54327 - 54333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Enserink, L. S. Price, T. Methi, M. Mahic, A. Sonnenberg, J. L. Bos, and K. Tasken The cAMP-Epac-Rap1 Pathway Regulates Cell Spreading and Cell Adhesion to Laminin-5 through the {alpha}3{beta}1 Integrin but Not the {alpha}6{beta}4 Integrin J. Biol. Chem., October 22, 2004; 279(43): 44889 - 44896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C Lenander, U J Roblick, J K Habermann, A Ost, K Tryggvason, and G Auer Laminin 5 {gamma}2 chain expression: a marker of early invasiveness in colorectal adenomas Mol. Pathol., December 1, 2003; 56(6): 342 - 346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Okamoto, S. Bachy, U. Odenthal, J. Bernaud, D. Rigal, H. Lortat-Jacob, N. Smyth, and P. Rousselle Normal Human Keratinocytes Bind to the {alpha}3LG4/5 Domain of Unprocessed Laminin-5 through the Receptor Syndecan-1 J. Biol. Chem., November 7, 2003; 278(45): 44168 - 44177. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Tsuruta, S. B. Hopkinson, K. D. Lane, M. E. Werner, V. L. Cryns, and J. C. R. Jones Crucial Role of the Specificity-determining Loop of the Integrin {beta}4 Subunit in the Binding of Cells to Laminin-5 and Outside-in Signal Transduction J. Biol. Chem., October 3, 2003; 278(40): 38707 - 38714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. G. Sathyanarayana, S. Toyooka, A. Padar, T. Takahashi, E. Brambilla, J. D. Minna, and A. F. Gazdar Epigenetic Inactivation of Laminin-5-encoding Genes in Lung Cancers Clin. Cancer Res., July 1, 2003; 9(7): 2665 - 2672. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. S. Udayakumar, M. L. Chen, E. L. Bair, D. C. von Bredow, A. E. Cress, R. B. Nagle, and G. T. Bowden Membrane Type-1-Matrix Metalloproteinase Expressed by Prostate Carcinoma Cells Cleaves Human Laminin-5 {beta}3 Chain and Induces Cell Migration Cancer Res., May 1, 2003; 63(9): 2292 - 2299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. P. Veitch, P. Nokelainen, K. A. McGowan, T.-T. Nguyen, N. E. Nguyen, R. Stephenson, W. N. Pappano, D. R. Keene, S. M. Spong, D. S. Greenspan, et al. Mammalian Tolloid Metalloproteinase, and Not Matrix Metalloprotease 2 or Membrane Type 1 Metalloprotease, Processes Laminin-5 in Keratinocytes and Skin J. Biol. Chem., April 25, 2003; 278(18): 15661 - 15668. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Gonzalez, M. Gonzales, G. S. Herron, U. Nagavarapu, S. B. Hopkinson, D. Tsuruta, and J. C. R. Jones Complex interactions between the laminin alpha 4 subunit and integrins regulate endothelial cell behavior in vitro and angiogenesis in vivo PNAS, December 10, 2002; 99(25): 16075 - 16080. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Gache, M. Allegra, C. Bodemer, A. Pisani-Spadafora, Y. de Prost, J. P. Ortonne, and G. Meneguzzi Genetic bases of severe junctional epidermolysis bullosa presenting spontaneous amelioration with aging Hum. Mol. Genet., October 1, 2001; 10(21): 2453 - 2461. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Gout, M. R. Jacquier-Sarlin, L. Rouard-Talbot, P. Rousselle, and M. R. Block RhoA-dependent Switch between alpha 2beta 1 and alpha 3beta 1 Integrins Is Induced by Laminin-5 during Early Stage of HT-29 Cell Differentiation Mol. Biol. Cell, October 1, 2001; 12(10): 3268 - 3281. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Gagnoux-Palacios, M. Allegra, F. Spirito, O. Pommeret, C. Romero, J.-p. Ortonne, and G. Meneguzzi The Short Arm of the Laminin {gamma}2 Chain Plays a Pivotal Role in the Incorporation of Laminin 5 into the Extracellular Matrix and in Cell Adhesion J. Cell Biol., May 14, 2001; 153(4): 835 - 850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F Decline and P Rousselle Keratinocyte migration requires alpha2beta1 integrin-mediated interaction with the laminin 5 gamma2 chain J. Cell Sci., January 2, 2001; 114(4): 811 - 823. [Abstract] [PDF]
|
|
|
|
 |
|
 B. L Ziober, S. S. Silverman Jr, and R. H. Kramer Adhesive Mechanisms Regulating Invasion and Metastasis in Oral Cancer Critical Reviews in Oral Biology & Medicine, January 1, 2001; 12(6): 499 - 510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Gonzales, B. Weksler, D. Tsuruta, R. D. Goldman, K. J. Yoon, S. B. Hopkinson, F. W. Flitney, and J. C. R. Jones Structure and Function of a Vimentin-associated Matrix Adhesion in Endothelial Cells Mol. Biol. Cell, January 1, 2001; 12(1): 85 - 100. [Abstract] [Full Text]
|
|
|
|
|
|
C. Catusse, M. Polette, C. Coraux, H. Burlet, and P. Birembaut Modified Basement Membrane Composition During Bronchopulmonary Tumor Progression J. Histochem. Cytochem., May 1, 2000; 48(5): 663 - 670. [Abstract] [Full Text]
|
|
|
|
|
|
P. H. Michelson, M. Tigue, and J. C.R. Jones Human Bronchial Epithelial Cells Secrete Laminin 5, Express Hemidesmosomal Proteins, and Assemble Hemidesmosomes J. Histochem. Cytochem., April 1, 2000; 48(4): 535 - 544. [Abstract] [Full Text]
|
|
|
|
|
|
N. Koshikawa, G. Giannelli, V. Cirulli, K. Miyazaki, and V. Quaranta Role of Cell Surface Metalloprotease Mt1-Mmp in Epithelial Cell Migration over Laminin-5 J. Cell Biol., February 7, 2000; 148(3): 615 - 624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Koch, P. F. Olson, A. Albus, W. Jin, D. D. Hunter, W. J. Brunken, R. E. Burgeson, and M.-F. Champliaud Characterization and Expression of the Laminin {gamma}3 Chain: A Novel, Non-Basement Membrane-associated, Laminin Chain J. Cell Biol., May 3, 1999; 145(3): 605 - 618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Giannelli, A. Pozzi, W. G. Stetler-Stevenson, H. A. Gardner, and V. Quaranta Expression of Matrix Metalloprotease-2-Cleaved Laminin-5 in Breast Remodeling Stimulated by Sex Steroids Am. J. Pathol., April 1, 1999; 154(4): 1193 - 1201. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G Cox, W P Steward, and K J O'Byrne The plasmin cascade and matrix metalloproteinases in non-small cell lung cancer Thorax, February 1, 1999; 54(2): 169 - 179. [Full Text]
|
|
|
|
|
|
M. Gonzales, K. Haan, S. E. Baker, M. Fitchmun, I. Todorov, S. Weitzman, and J. C.R. Jones A Cell Signal Pathway Involving Laminin-5, alpha 3beta 1 Integrin, and Mitogen-activated Protein Kinase Can Regulate Epithelial Cell Proliferation Mol. Biol. Cell, February 1, 1999; 10(2): 259 - 270. [Abstract] [Full Text]
|
|
|
|
|
|
M Grassi, G Moens, P Rousselle, J. Thiery, and J Jouanneau The SFL activity secreted by metastatic carcinoma cells is related to laminin 5 and mediates cell scattering in an integrin-independent manner J. Cell Sci., January 8, 1999; 112(15): 2511 - 2520. [Abstract] [PDF]
|
|
|
|
|
|
L. Goldfinger, S. Hopkinson, G. deHart, S Collawn, J. Couchman, and J. Jones The alpha3 laminin subunit, alpha6beta4 and alpha3beta1 integrin coordinately regulate wound healing in cultured epithelial cells and in the skin J. Cell Sci., January 8, 1999; 112(16): 2615 - 2629. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Zhou, A Nichols, A Wohlwend, I Bolon, and J. Vassalli Extracellular proteolysis alters tooth development in transgenic mice expressing urokinase-type plasminogen activator in the enamel organ Development, January 2, 1999; 126(5): 903 - 912. [Abstract] [PDF]
|
|
|
|
|
|
Y. Kikkawa, N. Sanzen, and K. Sekiguchi Isolation and Characterization of Laminin-10/11 Secreted by Human Lung Carcinoma Cells. LAMININ-10/11 MEDIATES CELL ADHESION THROUGH INTEGRIN alpha 3beta 1 J. Biol. Chem., June 19, 1998; 273(25): 15854 - 15859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. E. Goldfinger, M. S. Stack, and J. C.R. Jones Processing of Laminin-5 and Its Functional Consequences: Role of Plasmin and Tissue-type Plasminogen Activator J. Cell Biol., April 6, 1998; 141(1): 255 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Ferrigno, T. Virolle, M.-F. Galliano, N. Chauvin, J.-P. Ortonne, G. Meneguzzi, and D. Aberdam Murine Laminin alpha 3A and alpha 3B Isoform Chains Are Generated by Usage of Two Promoters and Alternative Splicing J. Biol. Chem., August 15, 1997; 272(33): 20502 - 20507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Stahl, S Weitzman, and J. Jones The role of laminin-5 and its receptors in mammary epithelial cell branching morphogenesis J. Cell Sci., January 1, 1997; 110(1): 55 - 63. [Abstract] [PDF]
|
|
|
|
|
|
S. Ghosh, R. Brown, J. C. R. Jones, S. M. Ellerbroek, and M. S. Stack Urinary-type Plasminogen Activator (uPA) Expression and uPA Receptor Localization Are Regulated by alpha 3beta 1 Integrin in Oral Keratinocytes J. Biol. Chem., July 28, 2000; 275(31): 23869 - 23876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. E. Goldfinger, L. Jiang, S. B. Hopkinson, M. S. Stack, and J. C. R. Jones Spatial Regulation and Activity Modulation of Plasmin by High Affinity Binding to the G domain of the alpha 3 Subunit of Laminin-5 J. Biol. Chem., November 3, 2000; 275(45): 34887 - 34893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Fujiwara, Y. Kikkawa, N. Sanzen, and K. Sekiguchi Purification and Characterization of Human Laminin-8. LAMININ-8 STIMULATES CELL ADHESION AND MIGRATION THROUGH alpha 3beta 1 AND alpha 6beta 1 INTEGRINS J. Biol. Chem., May 11, 2001; 276(20): 17550 - 17558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Spirito, S. Chavanas, C. Prost-Squarcioni, L. Pulkkinen, S. Fraitag, C. Bodemer, J.-P. Ortonne, and G. Meneguzzi Reduced Expression of the Epithelial Adhesion Ligand Laminin 5 in the Skin Causes Intradermal Tissue Separation J. Biol. Chem., May 25, 2001; 276(22): 18828 - 18835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shang, N. Koshikawa, S. Schenk, and V. Quaranta The LG3 Module of Laminin-5 Harbors a Binding Site for Integrin alpha 3beta 1 That Promotes Cell Adhesion, Spreading, and Migration J. Biol. Chem., August 24, 2001; 276(35): 33045 - 33053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. B. Robbins, Q. Lin, J. B. Goodnough, H. Tian, X. Chen, and P. A. Khavari In vivo restoration of laminin 5 beta 3 expression and function in junctional epidermolysis bullosa PNAS, April 24, 2001; 98(9): 5193 - 5198. [Abstract] [Full Text] [PDF]
|
|
