spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Frenz, L. M.
Right arrow Articles by Glover, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Frenz, L. M.
Right arrow Articles by Glover, D. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 109, Issue 11 2649-2660, Copyright © 1996 by Company of Biologists

JOURNAL ARTICLES

A maternal requirement for glutamine synthetase I for the mitotic cycles of syncytial Drosophila embryos

LM Frenz and DM Glover
Department of Anatomy and Physiology, University of Dundee, Scotland, UK.

We describe the maternal effect phenotype of a hypomorphic mutation in the Drosophila gene for glutamine synthetase I (GSI). The extent of development of embryos derived from homozygous mutant females is variable, although most mutant embryos fail to survive past germband elongation and none develop into larvae. These embryos are characterised by an increase in the number of yolk-like nuclei following nuclear migration to the cortex. These nuclei appear to fall into the interior of the embryo from the cortex at blastoderm. As they do so, the majority continue to show association with PCNA in synchrony with nuclei at the cortex, suggesting some continuity of the synchrony of DNA replication. However, the occurrence of nuclei that have lost cell cycle synchrony with their neighbours is not uncommon. Immunostaining of mutant embryos revealed a range of mitotic defects, ultimately resulting in nuclear fusion events, division failure or other mitotic abnormalities. A high proportion of these mitotic figures show chromatin bridging at anaphase and telophase consistent with progression through mitosis in the presence of incompletely replicated DNA. GSI is responsible for the ATP-dependent amination of glutamate to produce glutamine, which is required in the formation of amino acids, purines and pyrimidines. We discuss how the loss of glutamine could depress both protein and DNA synthesis and lead to a variety of mitotic defects in this embryonic system that lacks certain checkpoint controls.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 JCBHome page
G. J. Buttrick, L. M.A. Beaumont, J. Leitch, C. Yau, J. R. Hughes, and J. G. Wakefield
Akt regulates centrosome migration and spindle orientation in the early Drosophila melanogaster embryo
J. Cell Biol., February 6, 2008; 180(3): 537 - 548.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
T. Kidd, R. Abu-Shumays, A. Katzen, J. C. Sisson, G. Jimenez, S. Pinchin, W. Sullivan, and D. Ish-Horowicz
The {epsilon}-Subunit of Mitochondrial ATP Synthase Is Required for Normal Spindle Orientation During the Drosophila Embryonic Divisions
Genetics, June 1, 2005; 170(2): 697 - 708.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
B. S. Gim, J. M. Park, J. H. Yoon, C. Kang, and Y.-J. Kim
Drosophila Med6 Is Required for Elevated Expression of a Large but Distinct Set of Developmentally Regulated Genes
Mol. Cell. Biol., August 1, 2001; 21(15): 5242 - 5255.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
T. Megraw, K Li, L. Kao, and T. Kaufman
The centrosomin protein is required for centrosome assembly and function during cleavage in Drosophila
Development, January 7, 1999; 126(13): 2829 - 2839.
[Abstract] [PDF]

Home page
 DevelopmentHome page
J. Erickson and T. Cline
Key aspects of the primary sex determination mechanism are conserved across the genus Drosophila
Development, January 8, 1998; 125(16): 3259 - 3268.
[Abstract] [PDF]


© The Company of Biologists Ltd 1996