spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hime, G. R.
Right arrow Articles by Fuller, M. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hime, G. R.
Right arrow Articles by Fuller, M. T.

Journal of Cell Science, Vol 109, Issue 12 2779-2788, Copyright © 1996 by Company of Biologists

JOURNAL ARTICLES

Assembly of ring canals in the male germ line from structural components of the contractile ring

GR Hime, JA Brill and MT Fuller
Department of Developmental Biology, Stanford University School of Medicine, CA 94305, USA.

Stable intercellular bridges called ring canals form following incomplete cytokinesis, and interconnect mitotically or meiotically related germ cells. We show that ring canals in Drosophila melanogaster males are surprisingly different from those previously described in females. Mature ring canal walls in males lack actin and appear to derive directly from structural proteins associated with the contractile ring. Ring canal assembly in males, as in females, initiates during cytokinesis with the appearance of a ring of phosphotyrosine epitopes at the site of the contractile ring. Following constriction, actin and myosin II disappear. However, at least four proteins present at the contractile ring remain: the three septins (Pnut, Sep1 and Sep2) and anillin. In sharp contrast, in ovarian ring canals, septins have not been detected, anillin is lost from mature ring canals and filamentous actin is a major component. In both males and females, a highly branched vesicular structure, termed the fusome, interconnects developing germ cells via the ring canals and is thought to coordinate mitotic germ cell divisions. We show that, in males, unlike females, the fusome persists and enlarges following cessation of the mitotic divisions, developing additional branches during meiosis. During differentiation, the fusome and its associated ring canals localize to the distal tip of the elongating spermatids.


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
E. Nicolas, N. Chenouard, J.-C. Olivo-Marin, and A. Guichet
A Dual Role for Actin and Microtubule Cytoskeleton in the Transport of Golgi Units from the Nurse Cells to the Oocyte Across Ring Canals
Mol. Biol. Cell, January 1, 2009; 20(1): 556 - 568.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. V. Silverman-Gavrila, K. G. Hales, and A. Wilde
Anillin-mediated Targeting of Peanut to Pseudocleavage Furrows Is Regulated by the GTPase Ran
Mol. Biol. Cell, September 1, 2008; 19(9): 3735 - 3744.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. Barreau, E. Benson, E. Gudmannsdottir, F. Newton, and H. White-Cooper
Post-meiotic transcription in Drosophila testes
Development, June 1, 2008; 135(11): 1897 - 1902.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H.-C. Wei, J. Rollins, L. Fabian, M. Hayes, G. Polevoy, C. Bazinet, and J. A. Brill
Depletion of plasma membrane PtdIns(4,5)P2 reveals essential roles for phosphoinositides in flagellar biogenesis
J. Cell Sci., April 1, 2008; 121(7): 1076 - 1084.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
N. Dyer, E. Rebollo, P. Dominguez, N. Elkhatib, P. Chavrier, L. Daviet, C. Gonzalez, and M. Gonzalez-Gaitan
Spermatocyte cytokinesis requires rapid membrane addition mediated by ARF6 on central spindle recycling endosomes
Development, December 15, 2007; 134(24): 4437 - 4447.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. G. Giansanti, G. Belloni, and M. Gatti
Rab11 Is Required for Membrane Trafficking and Actomyosin Ring Constriction in Meiotic Cytokinesis of Drosophila Males
Mol. Biol. Cell, December 1, 2007; 18(12): 5034 - 5047.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. C. Baker and M. T. Fuller
Translational control of meiotic cell cycle progression and spermatid differentiation in male germ cells by a novel eIF4G homolog
Development, August 1, 2007; 134(15): 2863 - 2869.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
C. Rathke, W. M. Baarends, S. Jayaramaiah-Raja, M. Bartkuhn, R. Renkawitz, and R. Renkawitz-Pohl
Transition from a nucleosome-based to a protamine-based chromatin configuration during spermiogenesis in Drosophila
J. Cell Sci., May 1, 2007; 120(9): 1689 - 1700.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
T. Krishnamoorthy, X. Chen, J. Govin, W. L. Cheung, J. Dorsey, K. Schindler, E. Winter, C. D. Allis, V. Guacci, S. Khochbin, et al.
Phosphorylation of histone H4 Ser1 regulates sporulation in yeast and is conserved in fly and mouse spermatogenesis
Genes & Dev., September 15, 2006; 20(18): 2580 - 2592.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. Casper and M. Van Doren
The control of sexual identity in the Drosophila germline
Development, August 1, 2006; 133(15): 2783 - 2791.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. K. Gatt and D. M. Glover
The Drosophila phosphatidylinositol transfer protein encoded by vibrator is essential to maintain cleavage-furrow ingression in cytokinesis
J. Cell Sci., June 1, 2006; 119(11): 2225 - 2235.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. P. Greenbaum, W. Yan, M.-H. Wu, Y.-N. Lin, J. E. Agno, M. Sharma, R. E. Braun, A. Rajkovic, and M. M. Matzuk
TEX14 is essential for intercellular bridges and fertility in male mice
PNAS, March 28, 2006; 103(13): 4982 - 4987.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Zavortink, N. Contreras, T. Addy, A. Bejsovec, and R. Saint
Tum/RacGAP50C provides a critical link between anaphase microtubules and the assembly of the contractile ring in Drosophila melanogaster
J. Cell Sci., November 15, 2005; 118(22): 5381 - 5392.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. M. Field, M. Coughlin, S. Doberstein, T. Marty, and W. Sullivan
Characterization of anillin mutants reveals essential roles in septin localization and plasma membrane integrity
Development, June 15, 2005; 132(12): 2849 - 2860.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
T.-K. Ju and F.-L. Huang
MSAP, the Meichroacidin Homolog of Carp (Cyprinus carpio), Differs from the Rodent Counterpart in Germline Expression and Involves Flagellar Differentiation
Biol Reprod, November 1, 2004; 71(5): 1419 - 1429.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
V. Naim, S. Imarisio, F. Di Cunto, M. Gatti, and S. Bonaccorsi
Drosophila Citron Kinase Is Required for the Final Steps of Cytokinesis
Mol. Biol. Cell, November 1, 2004; 15(11): 5053 - 5063.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
C. Schulz, A. A. Kiger, S. I. Tazuke, Y. M. Yamashita, L. C. Pantalena-Filho, D. L. Jones, C. G. Wood, and M. T. Fuller
A Misexpression Screen Reveals Effects of bag-of-marbles and TGF{beta} Class Signaling on the Drosophila Male Germ-Line Stem Cell Lineage
Genetics, June 1, 2004; 167(2): 707 - 723.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
C. Brawley and E. Matunis
Regeneration of Male Germline Stem Cells by Spermatogonial Dedifferentiation in Vivo
Science, May 28, 2004; 304(5675): 1331 - 1334.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. G. Giansanti, R. M. Farkas, S. Bonaccorsi, D. L. Lindsley, B. T. Wakimoto, M. T. Fuller, and M. Gatti
Genetic Dissection of Meiotic Cytokinesis in Drosophila Males
Mol. Biol. Cell, May 1, 2004; 15(5): 2509 - 2522.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Ghosh-Roy, M. Kulkarni, V. Kumar, S. Shirolikar, and K. Ray
Cytoplasmic Dynein-Dynactin Complex Is Required for Spermatid Growth but Not Axoneme Assembly in Drosophila
Mol. Biol. Cell, May 1, 2004; 15(5): 2470 - 2483.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
M. Kinoshita
Assembly of Mammalian Septins
J. Biochem., October 1, 2003; 134(4): 491 - 496.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
F. D. Cunto, S. Imarisio, P. Camera, C. Boitani, F. Altruda, and L. Silengo
Essential role of citron kinase in cytokinesis of spermatogenic precursors
J. Cell Sci., March 14, 2003; 115(24): 4819 - 4826.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. D. Rogat and K. G. Miller
A role for myosin VI in actin dynamics at sites of membrane remodeling during Drosophila spermatogenesis
J. Cell Sci., March 14, 2003; 115(24): 4855 - 4865.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
S. I. Tazuke, C. Schulz, L. Gilboa, M. Fogarty, A. P. Mahowald, A. Guichet, A. Ephrussi, C. G. Wood, R. Lehmann, and M. T. Fuller
A germline-specific gap junction protein required for survival of differentiating early germ cells
Development, March 7, 2003; 129(10): 2529 - 2539.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
V. Barbosa, M. Gatt, E. Rebollo, C. Gonzalez, and D. M. Glover
Drosophila dd4 mutants reveal that {gamma}TuRC is required to maintain juxtaposed half spindles in spermatocytes
J. Cell Sci., March 1, 2003; 116(5): 929 - 941.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. Xu, G. L. Boulianne, and W. S. Trimble
Drosophila syntaxin 16 is a Q-SNARE implicated in Golgi dynamics
J. Cell Sci., January 12, 2002; 115(23): 4447 - 4455.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. Schulz, C. G. Wood, D. L. Jones, S. I. Tazuke, and M. T. Fuller
Signaling from germ cells mediated by the rhomboid homolog stet organizes encapsulation by somatic support cells
Development, January 10, 2002; 129(19): 4523 - 4534.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. G. Riparbelli, G. Callaini, D. M. Glover, and M. d. C. Avides
A requirement for the Abnormal Spindle protein to organise microtubules of the central spindle for cytokinesis in Drosophila
J. Cell Sci., January 3, 2002; 115(5): 913 - 922.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
N. Tulina and E. Matunis
Control of Stem Cell Self-Renewal in Drosophila Spermatogenesis by JAK-STAT Signaling
Science, December 21, 2001; 294(5551): 2546 - 2549.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. D. Schnorr, R. Holdcraft, B. Chevalier, and C. A. Berg
Ras1 Interacts With Multiple New Signaling and Cytoskeletal Loci in Drosophila Eggshell Patterning and Morphogenesis
Genetics, October 1, 2001; 159(2): 609 - 622.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. C. Adam, J. R. Pringle, and M. Peifer
Evidence for Functional Differentiation among Drosophila Septins in Cytokinesis and Cellularization
Mol. Biol. Cell, September 1, 2000; 11(9): 3123 - 3135.
[Abstract] [Full Text]

Home page
 JCBHome page
K. Oegema, M. S. Savoian, T. J. Mitchison, and C. M. Field
Functional Analysis of a Human Homologue of the Drosophila Actin Binding Protein Anillin Suggests a Role in Cytokinesis
J. Cell Biol., August 7, 2000; 150(3): 539 - 552.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. Brill, G. Hime, M Scharer-Schuksz, and M. Fuller
A phospholipid kinase regulates actin organization and intercellular bridge formation during germline cytokinesis
Development, January 9, 2000; 127(17): 3855 - 3864.
[Abstract] [PDF]

Home page
 Mol. Biol. CellHome page
J. L. Hicks, W.-M. Deng, A. D. Rogat, K. G. Miller, and M. Bownes
Class VI Unconventional Myosin is Required for Spermatogenesis in Drosophila
Mol. Biol. Cell, December 1, 1999; 10(12): 4341 - 4353.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
M. Giansanti, S Bonaccorsi, and M Gatti
The role of anillin in meiotic cytokinesis of Drosophila males
J. Cell Sci., January 7, 1999; 112(14): 2323 - 2334.
[Abstract] [PDF]

Home page
 DevelopmentHome page
S Hinson and R. Nagoshi
Regulatory and functional interactions between the somatic sex regulatory gene transformer and the germline genes ovo and ovarian tumor
Development, January 2, 1999; 126(5): 861 - 871.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
T. T.-L. Tang, S. E. Bickel, L. M. Young, and T. L. Orr-Weaver
Maintenance of sister-chromatid cohesion at the centromere by the Drosophila MEI-S332 protein
Genes & Dev., December 15, 1998; 12(24): 3843 - 3856.
[Abstract] [Full Text]

Home page
 JCBHome page
M. Carmena, M. G. Riparbelli, G. Minestrini, A. M. Tavares, R. Adams, G. Callaini, and D. M. Glover
Drosophila Polo Kinase Is Required for Cytokinesis
J. Cell Biol., November 2, 1998; 143(3): 659 - 671.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S. Bonaccorsi, M. G. Giansanti, and M. Gatti
Spindle Self-organization and Cytokinesis During Male Meiosis in asterless Mutants of Drosophila melanogaster
J. Cell Biol., August 10, 1998; 142(3): 751 - 761.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
M. G. Giansanti, S. Bonaccorsi, B. Williams, E. V. Williams, C. Santolamazza, M. L. Goldberg, and M. Gatti
Cooperative interactions between the central spindle and the contractile ring during Drosophila cytokinesis
Genes & Dev., February 1, 1998; 12(3): 396 - 410.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
A Santel, N Blumer, M Kampfer, and R Renkawitz-Pohl
Flagellar mitochondrial association of the male-specific Don Juan protein in Drosophila spermatozoa
J. Cell Sci., January 11, 1998; 111(22): 3299 - 3309.
[Abstract] [PDF]

Home page
 DevelopmentHome page
P Gonczy, E Matunis, and S DiNardo
bag-of-marbles and benign gonial cell neoplasm act in the germline to restrict proliferation during Drosophila spermatogenesis
Development, January 11, 1997; 124(21): 4361 - 4371.
[Abstract] [PDF]

Home page
 DevelopmentHome page
E Matunis, J Tran, P Gonczy, K Caldwell, and S DiNardo
punt and schnurri regulate a somatically derived signal that restricts proliferation of committed progenitors in the germline
Development, January 11, 1997; 124(21): 4383 - 4391.
[Abstract] [PDF]


© The Company of Biologists Ltd 1996