|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 109, Issue 12 2895-2904, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
T Sasaki, H Wiedemann, M Matzner, ML Chu and R Timpl
Max-Planck-Institut fur Biochemie, Martinsried, Germany.
The extracellular matrix protein fibulin-2 was shown to be a typical product of cultured human and mouse fibroblasts by several immunological assays. It is secreted and deposited in cells and tissues as a disulfide-bonded oligomer identical in size to the previously described recombinant fibulin-2. Most of the fibroblast fibulin-2 is deposited into a dense fibrillar meshwork which requires treatment with EDTA and/or 6 M urea for solubilization. Fibulin-2 and fibronectin are synthesized at equivalent levels and both colocalize in the fibrils as shown by immunofluorescence. Metabolic labelling and pulse-chase studies demonstrated fibulin-2 oligomers in detergent extracts of cells and their rapid translocation to extracellular EDTA-sensitive assembly forms. Unlike for fibronectin and fibulin-1 only a little fibulin-2 was found in the cell culture medium. Immunogold staining of confluent human fibroblasts showed localization of fibulin-2 to a fine meshwork or bundles of amorphous microfibrils in the matrix. This also demonstrated a distinct colocalization of fibulin-2 and fibronectin at the electron microscope level, indicating that the interaction between these two protein shown in in vitro assays may also exist in situ. No distinct colocalization of both proteins could, however, be observed with cross-striated fibrils of collagen I and collagen VI microfibrils.
This article has been cited by other articles:
|
|
 |
|
  F.-X. Sicot, T. Tsuda, D. Markova, J. F. Klement, M. Arita, R.-Z. Zhang, T.-C. Pan, R. P. Mecham, D. E. Birk, and M.-L. Chu Fibulin-2 Is Dispensable for Mouse Development and Elastic Fiber Formation Mol. Cell. Biol., February 1, 2008; 28(3): 1061 - 1067. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-H. Yi, D. J. Smith, W. W. West, and M. A. Hollingsworth Loss of Fibulin-2 Expression Is Associated with Breast Cancer Progression Am. J. Pathol., May 1, 2007; 170(5): 1535 - 1545. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Kobayashi, G. Kostka, J. H. O. Garbe, D. R. Keene, H. P. Bachinger, F.-G. Hanisch, D. Markova, T. Tsuda, R. Timpl, M.-L. Chu, et al. A Comparative Analysis of the Fibulin Protein Family: BIOCHEMICAL CHARACTERIZATION, BINDING INTERACTIONS, AND TISSUE LOCALIZATION J. Biol. Chem., April 20, 2007; 282(16): 11805 - 11816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Gersdorff, E. Kohfeldt, T. Sasaki, R. Timpl, and N. Miosge Laminin {gamma}3 Chain Binds to Nidogen and Is Located in Murine Basement Membranes J. Biol. Chem., June 10, 2005; 280(23): 22146 - 22153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Sottile and J. Chandler Fibronectin Matrix Turnover Occurs through a Caveolin-1-dependent Process Mol. Biol. Cell, February 1, 2005; 16(2): 757 - 768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Sottile and D. C. Hocking Fibronectin Polymerization Regulates the Composition and Stability of Extracellular Matrix Fibrils and Cell-Matrix Adhesions Mol. Biol. Cell, October 1, 2002; 13(10): 3546 - 3559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Loeys, L. Van Maldergem, G. Mortier, P. Coucke, S. Gerniers, J.-M. Naeyaert, and A. De Paepe Homozygosity for a missense mutation in fibulin-5 (FBLN5) results in a severe form of cutis laxa Hum. Mol. Genet., September 1, 2002; 11(18): 2113 - 2118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Isogai, A. Aspberg, D. R. Keene, R. N. Ono, D. P. Reinhardt, and L. Y. Sakai Versican Interacts with Fibrillin-1 and Links Extracellular Microfibrils to Other Connective Tissue Networks J. Biol. Chem., February 1, 2002; 277(6): 4565 - 4572. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Willem, N. Miosge, W. Halfter, N. Smyth, I. Jannetti, E. Burghart, R. Timpl, and U. Mayer Specific ablation of the nidogen-binding site in the laminin {gamma}1 chain interferes with kidney and lung development Development, January 6, 2002; 129(11): 2711 - 2722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Kostka, R. Giltay, W. Bloch, K. Addicks, R. Timpl, R. Fassler, and M.-L. Chu Perinatal Lethality and Endothelial Cell Abnormalities in Several Vessel Compartments of Fibulin-1-Deficient Mice Mol. Cell. Biol., October 15, 2001; 21(20): 7025 - 7034. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. SASAKI, E. HOHENESTER, R.-Z. ZHANG, S. GOTTA, M. C. SPEER, R. TANDAN, R. TIMPL, and M.-L. CHU A Bethlem myopathy Gly to Glu mutation in the von Willebrand factor A domain N2 of the collagen {alpha}3(VI) chain interferes with protein folding FASEB J, April 1, 2000; 14(5): 761 - 768. [Abstract] [Full Text]
|
|
|
|
|
|
N. MIOSGE, T. SASAKI, and R. TIMPL Angiogenesis inhibitor endostatin is a distinct component of elastic fibers in vessel walls FASEB J, October 1, 1999; 13(13): 1743 - 1750. [Abstract] [Full Text]
|
|
|
|
|
|
J. F. Talts, T. Sasaki, N. Miosge, W. Gohring, K. Mann, R. Mayne, and R. Timpl Structural and Functional Analysis of the Recombinant G Domain of the Laminin alpha 4 Chain and Its Proteolytic Processing in Tissues J. Biol. Chem., November 3, 2000; 275(45): 35192 - 35199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. I. Olin, M. Morgelin, T. Sasaki, R. Timpl, D. Heinegard, and A. Aspberg The Proteoglycans Aggrecan and Versican Form Networks with Fibulin-2 through Their Lectin Domain Binding J. Biol. Chem., January 5, 2001; 276(2): 1253 - 1261. [Abstract] [Full Text] [PDF]
|
|
