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Journal of Cell Science, Vol 109, Issue 13 3013-3023, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
AJ Zhu and FM Watt
Keratinocyte Laboratory, Imperial Cancer Research Fund, London, UK.
Cell adhesion molecules are not only required for maintenance of tissue integrity, but also regulate many aspects of cell behaviour, including growth and differentiation. While the regulatory functions of integrin extracellular matrix receptors in keratinocytes are well established, such functions have not been investigated for the primary receptors that mediate keratinocyte intercellular adhesion, the cadherins. To examine cadherin function in normal human epidermal keratinocytes we used a retroviral vector to introduce a dominant negative E-cadherin mutant, consisting of the extracellular domain of H-2Kd and the transmembrane and cytoplasmic domains of E-cadherin. As a control a vector containing the same construct, but with the catenin binding site destroyed, was prepared. High levels of expression of the constructs were achieved; the dominant negative mutant, but not the control, formed complexes with alpha-, beta- and gamma-catenin. In cells expressing the dominant negative mutant there was a 5-fold decrease in the level of endogenous cadherins and a 3-fold increase in the level of beta-catenin. Cell-cell adhesion and stratification were inhibited by the dominant negative mutant and desmosome formation was reduced. Expression of the mutant resulted in reduced levels of the alpha 2 beta 1 and alpha 3 beta 1 integrins and increased cell motility, providing further evidence for cross-talk between cadherins and the beta 1 integrins. In view of the widely documented loss of E-cadherin in keratinocyte tumours it was surprising that the dominant negative mutant had an inhibitory effect on keratinocyte proliferation and stimulated terminal differentiation even under conditions in which intercellular adhesion was prevented. These results establish a role for cadherins in regulating keratinocyte growth and differentiation and raise interesting questions as to the relative importance of cell adhesion-dependent and -independent mechanisms.
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|
|
|
|
|
|
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|
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|
|
|
|
|
|
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|
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|
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|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
 |
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
A. Zhu and F. Watt beta-catenin signalling modulates proliferative potential of human epidermal keratinocytes independently of intercellular adhesion Development, January 5, 1999; 126(10): 2285 - 2298. [Abstract] [PDF]
|
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|
|
|
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G. I. Gallicano, P. Kouklis, C. Bauer, M. Yin, V. Vasioukhin, L. Degenstein, and E. Fuchs Desmoplakin Is Required Early in Development for Assembly of Desmosomes and Cytoskeletal Linkage J. Cell Biol., December 28, 1998; 143(7): 2009 - 2022. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Tselepis, M. Chidgey, A. North, and D. Garrod Desmosomal adhesion inhibits invasive behavior PNAS, July 7, 1998; 95(14): 8064 - 8069. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Kantak and R. H. Kramer E-cadherin Regulates Anchorage-independent Growth and Survival in Oral Squamous Cell Carcinoma Cells J. Biol. Chem., July 3, 1998; 273(27): 16953 - 16961. [Abstract] [Full Text] [PDF]
|
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|
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A. Huttenlocher, M. Lakonishok, M. Kinder, S. Wu, T. Truong, K. A. Knudsen, and A. F. Horwitz Integrin and Cadherin Synergy Regulates Contact Inhibition of Migration and Motile Activity J. Cell Biol., April 20, 1998; 141(2): 515 - 526. [Abstract] [Full Text] [PDF]
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B. Harvat, A Wang, P Seth, and A. Jetten Up-regulation of p27Kip1, p21WAF1/Cip1 and p16Ink4a is associated with, but not sufficient for, induction of squamous differentiation J. Cell Sci., January 5, 1998; 111(9): 1185 - 1196. [Abstract] [PDF]
|
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|
|
|
|
S. Norvell and K. Green Contributions of extracellular and intracellular domains of full length and chimeric cadherin molecules to junction assembly in epithelial cells J. Cell Sci., January 5, 1998; 111(9): 1305 - 1318. [Abstract] [PDF]
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S. V. Litvinov, M. Balzar, M. J. Winter, H. A.M. Bakker, I. H. B.-d. Bruijn, F. Prins, G. J. Fleuren, and S. O. Warnaar Epithelial Cell Adhesion Molecule (Ep-CAM) Modulates Cell-Cell Interactions Mediated by Classic Cadherins J. Cell Biol., December 1, 1997; 139(5): 1337 - 1348. [Abstract] [Full Text] [PDF]
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 J.-P. Moles and F. M. Watt The Epidermal Stem Cell Compartment: Variation in Expression Levels of E-Cadherin and Catenins Within the Basal Layer of Human Epidermis J. Histochem. Cytochem., June 1, 1997; 45(6): 867 - 874. [Abstract] [Full Text] [PDF]
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