|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 109, Issue 13 3103-3112, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
A Kalt and M Schliwa
Adolf-Butenandt-Institut, Zellbiologie, Ludwig-Maximimilians-Universitat Munchen, Germany.
The microtubule-organizing center of D. discoideum is a nucleus-associated body (NAB) that consists of a multilayered, box-shaped core embedded in an amorphous corona from which the microtubules emerge. The composition of the NAB is still largely unresolved. Here we have examined a high molecular mass component of the NAB which was identified by a monoclonal antibody raised against isolated nucleus/NAB complexes. This antibody recognized a 350 kDa component which is immunologically related to the D. discoideum heavy chain of myosin II. The 350 kDa antigen was localized only at the NAB in interphase cells, while in mitotic cells it may also be found in the vicinity of the NAB as well as in association with the mitotic spindle. Immunogold labeling experiments showed that the protein is part of the NAB corona. This association was not destroyed by treatment with 2 M urea or 0.6 M KCl. The 350 kDa antigen was part of the thiabendazole-induced cytoplasmic microtubule-organizing centers. A direct role in the polymerization of tubulin could not be determined in an in vitro microtubule nucleation assay, whereas antibody electroporation of live cells appeared to interfere with the generation of a normal microtubule system in a subset of cells. Our observations suggest that the 350 kDa antigen is a structural component of the NAB corona which could be involved in its stabilization.
This article has been cited by other articles:
|
|
 |
|
  M. Kaller, U. Euteneuer, and W. Nellen Differential Effects of Heterochromatin Protein 1 Isoforms on Mitotic Chromosome Distribution and Growth in Dictyostelium discoideum Eukaryot. Cell, March 1, 2006; 5(3): 530 - 543. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Graf DdNek2, the first non-vertebrate homologue of human Nek2, is involved in the formation of microtubule-organizing centers J. Cell Sci., January 5, 2002; 115(9): 1919 - 1929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R Graf, C Daunderer, and M Schliwa Dictyostelium DdCP224 is a microtubule-associated protein and a permanent centrosomal resident involved in centrosome duplication J. Cell Sci., January 5, 2000; 113(10): 1747 - 1758. [Abstract] [PDF]
|
|
|
|
|
|
B. Schnackenberg, D. Hull, R. Balczon, and R. Palazzo Reconstitution of microtubule nucleation potential in centrosomes isolated from Spisula solidissima oocytes J. Cell Sci., January 3, 2000; 113(6): 943 - 953. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Ma, L. Trivinos-Lagos, R. Graf, and R. L. Chisholm Dynein Intermediate Chain Mediated Dynein-Dynactin Interaction Is Required for Interphase Microtubule Organization and Centrosome Replication and Separation in Dictyostelium J. Cell Biol., December 13, 1999; 147(6): 1261 - 1274. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Brachat, J. V. Kilmartin, A. Wach, and P. Philippsen Saccharomyces cerevisiae Cells with Defective Spindle Pole Body Outer Plaques Accomplish Nuclear Migration via Half-Bridge-organized Microtubules Mol. Biol. Cell, May 1, 1998; 9(5): 977 - 991. [Abstract] [Full Text]
|
|
|
|
|
|
U Euteneuer, R Graf, E Kube-Granderath, and M Schliwa Dictyostelium gamma-tubulin: molecular characterization and ultrastructural localization J. Cell Sci., January 2, 1998; 111(3): 405 - 412. [Abstract] [PDF]
|
|
|
|
 |
|
 M. Ueda, R. Graf, H. K. MacWilliams, M. Schliwa, and U. Euteneuer Centrosome positioning and directionality of cell movements PNAS, September 2, 1997; 94(18): 9674 - 9678. [Abstract] [Full Text] [PDF]
|
|
