|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 109, Issue 3 591-607, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
MR Paddy, H Saumweber, DA Agard and JW Sedat
Center for Structural Biology and Department of Anatomy and Cell Biology, University of Florida, Gainesville, FL 32610-0235, USA.
Time-resolved, two-component, three-dimensional fluorescence light microscopy imaging in living Drosophila early embryos is used to demonstrate that a large fraction of the nuclear envelope lamins remain localized to a rim in the nuclear periphery until well into metaphase. The process of lamin delocalization and dispersal, typical of 'open' forms of mitosis, does not begin until about the time the final, metaphase geometry of the mitotic spindle is attained. Lamin dispersal is completed about the time that the chromosomal movements of anaphase begin. This pattern of nuclear lamina breakdown appears to be intermediate between traditional designations of 'open' and 'closed' mitoses. These results thus clarify earlier observations of lamins in mitosis in fixed Drosophila early embryos, clearly showing that the observed lamin localization does not result from a structurally defined 'spindle envelope' that persists throughout mitosis. During this extended time interval of lamin localization in the nuclear periphery, the lamina undergoes an extensive series of structural rearrangements that are closely coupled to, and likely driven by, the movements of the centrosomes and microtubules that produce the mitotic spindle. Furthermore, throughout this time the nuclear envelope structure is permeable to large macromolecules, which are excluded in interphase. While the functional significance of these structural dynamics is not yet clear, it is consistent with a functional role for the lamina in mitotic spindle formation.
This article has been cited by other articles:
|
|
 |
|
  K. R. Katsani, R. E. Karess, N. Dostatni, and V. Doye In Vivo Dynamics of Drosophila Nuclear Envelope Components Mol. Biol. Cell, September 1, 2008; 19(9): 3652 - 3666. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. P. C. De Souza and S. A. Osmani Mitosis, Not Just Open or Closed Eukaryot. Cell, September 1, 2007; 6(9): 1521 - 1527. [Full Text] [PDF]
|
|
|
|
 |
|
 H. Parry, A. McDougall, and M. Whitaker Microdomains bounded by endoplasmic reticulum segregate cell cycle calcium transients in syncytial Drosophila embryos J. Cell Biol., October 10, 2005; 171(1): 47 - 59. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Wasser and W. Chia The Drosophila EAST protein associates with a nuclear remnant during mitosis and constrains chromosome mobility J. Cell Sci., May 1, 2003; 116(9): 1733 - 1743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Timinszky, L. Tirian, F. T. Nagy, G. Toth, A. Perczel, Z. Kiss-Laszlo, I. Boros, P. R. Clarke, and J. Szabad The importin-{beta} P446L dominant-negative mutant protein loses RanGTP binding ability and blocks the formation of intact nuclear envelope J. Cell Sci., April 15, 2002; 115(8): 1675 - 1687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kiseleva, S. Rutherford, L. M. Cotter, T. D. Allen, and M. W. Goldberg Steps of nuclear pore complex disassembly and reassembly during mitosis in early Drosophila embryos J. Cell Sci., March 12, 2002; 114(20): 3607 - 3618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. C. de Noronha, M. P. Sherman, H. W. Lin, M. V. Cavrois, R. D. Moir, R. D. Goldman, and W. C. Greene Dynamic Disruptions in Nuclear Envelope Architecture and Integrity Induced by HIV-1 Vpr Science, November 2, 2001; 294(5544): 1105 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kuhfittig, J. Szabad, G. Schotta, J. Hoffmann, E. Máthé, and G. Reuter pitkinD, a Novel Gain-of-Function Enhancer of Position-Effect Variegation, Affects Chromatin Regulation During Oogenesis and Early Embryogenesis in Drosophila Genetics, March 1, 2001; 157(3): 1227 - 1244. [Abstract] [Full Text]
|
|
|
|
|
|
D. L. Walker, D. Wang, Y. Jin, U. Rath, Y. Wang, J. Johansen, and K. M. Johansen Skeletor, a Novel Chromosomal Protein That Redistributes during Mitosis Provides Evidence for the Formation of a Spindle Matrix J. Cell Biol., December 18, 2000; 151(7): 1401 - 1412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lippai, L. Tirián, I. Boros, J. Mihály, M. Erdélyi, I. Belecz, E. Máthé, J. Pósfai, A. Nagy, A. Udvardy, et al. The Ketel Gene Encodes a Drosophila Homologue of Importin-{beta} Genetics, December 1, 2000; 156(4): 1889 - 1900. [Abstract] [Full Text]
|
|
|
|
|
|
K. K. Lee, Y. Gruenbaum, P. Spann, J. Liu, and K. L. Wilson C. elegans Nuclear Envelope Proteins Emerin, MAN1, Lamin, and Nucleoporins Reveal Unique Timing of Nuclear Envelope Breakdown during Mitosis Mol. Biol. Cell, September 1, 2000; 11(9): 3089 - 3099. [Abstract] [Full Text]
|
|
|
|
|
|
E. M.M. Manders, H. Kimura, and P. R. Cook Direct Imaging of DNA in Living Cells Reveals the Dynamics of Chromosome Formation J. Cell Biol., March 8, 1999; 144(5): 813 - 822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P Collas Sequential PKC- and Cdc2-mediated phosphorylation events elicit zebrafish nuclear envelope disassembly J. Cell Sci., January 3, 1999; 112(6): 977 - 987. [Abstract] [PDF]
|
|
|
|
|
|
P Collas Nuclear envelope disassembly in mitotic extract requires functional nuclear pores and a nuclear lamina J. Cell Sci., January 5, 1998; 111(9): 1293 - 1303. [Abstract] [PDF]
|
|
|
|
|
|
S. Georgatos, A Pyrpasopoulou, and P. Theodoropoulos Nuclear envelope breakdown in mammalian cells involves stepwise lamina disassembly and microtubule-drive deformation of the nuclear membrane J. Cell Sci., January 9, 1997; 110(17): 2129 - 2140. [Abstract] [PDF]
|
|
|
|
|
|
G Zimowska, J. Aris, and M. Paddy A Drosophila Tpr protein homolog is localized both in the extrachromosomal channel network and to nuclear pore complexes J. Cell Sci., January 4, 1997; 110(8): 927 - 944. [Abstract] [PDF]
|
|
