|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 109, Issue 5 1105-1112, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
J Renzing, S Hansen and DP Lane
Department of Biochemistry, University of Dundee, UK.
In many vertebrate cells exposure to ultraviolet light lead to a dramatic increase in the cellular levels of the tumour suppressor protein p53, followed by a biological response of either growth arrest or programmed cell death. Ultraviolet light can be absorbed directly by cellular macromolecules, leading to photochemical modification of DNA and proteins. Additionally, it also causes free radical formation, resulting in oxidative stress. Whereas ultraviolet light and ionizing radiation both induce DNA lesions which trigger an activation of the p53 pathway, the magnitude of the p53 response elicited by ionizing radiation is comparatively low. Following irradiation with ultraviolet light two populations of p53-reactive cells are induced: a population accumulating high levels of p53 protein and a population with comparatively low levels of p53, similar in magnitude to the p53 response following ionizing radiation. Pretreatment of cells with N-acetylcysteine, an agent known to counteract oxidative stress, attenuates the cellular p53 response to ultraviolet light by reducing the number of cells with high p53 levels but does not affect the response to ionizing radiation. We demonstrate that N-acetylcysteine pretreatment does not prevent the inflicted DNA damage and therefore conclude that oxidative stress is a causative agent in the ultraviolet light activation of the p53 pathway.
This article has been cited by other articles:
|
|
 |
|
  S. H. K. Wong, R. S. M. Shih, N. W. Schoene, and K. Y. Lei Zinc-induced G2/M blockage is p53 and p21 dependent in normal human bronchial epithelial cells Am J Physiol Cell Physiol, June 1, 2008; 294(6): C1342 - C1349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Lesser, V. A. Kruse, and T. M. Barry Exposure to ultraviolet radiation causes apoptosis in developing sea urchin embryos J. Exp. Biol., November 15, 2003; 206(22): 4097 - 4103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lesser, J. Farrell, and C. Walker Oxidative stress, DNA damage and p53 expression in the larvae of atlantic cod (Gadus morhua) exposed to ultraviolet (290-400 nm) radiation J. Exp. Biol., January 1, 2001; 204(1): 157 - 164. [Abstract] [PDF]
|
|
|
|
 |
|
 X. W. Wang, Q. Zhan, J. D. Coursen, M. A. Khan, H. U. Kontny, L. Yu, M. C. Hollander, P. M. O'Connor, A. J. Fornace Jr., and C. C. Harris GADD45 induction of a G2/M cell cycle checkpoint PNAS, March 30, 1999; 96(7): 3706 - 3711. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Bray, C. Schorl, and P. A. Hall The Challenge of p53: Linking Biochemistry, Biology, and Patient Management Stem Cells, July 1, 1998; 16(4): 248 - 260. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Amdjadi and B. M. Sefton Ultraviolet Light-induced Stimulation of the JNK Mitogen-activated Protein Kinase in the Absence of Src Family Tyrosine Kinase Activation J. Biol. Chem., July 14, 2000; 275(29): 22520 - 22525. [Abstract] [Full Text] [PDF]
|
|
