Journal of Cell Science, Vol 109, Issue 6 1265-1274, Copyright © 1996 by Company of Biologists

JOURNAL ARTICLES

Identification of a novel member of the Rab8 family from the rat basophilic leukaemia cell line, RBL.2H3

J Armstrong, N Thompson, JH Squire, J Smith, B Hayes and R Solari
Cell Biology Unit, Glaxo Wellcome Medicines Research Centre, Stevenage, Hertfordshire, UK.

We describe the cloning of a cDNA from the rat basophilic leukaemia cell line (RBL.2H3) encoding a novel member of the Rab family of small GTP binding proteins. The novel clone, which we call Rab8b, is most highly related to the Rab8 family with substantial divergence in the variable C-terminal domain. Northern blot analysis reveals highest levels of expression of Rab8b in the spleen, testis and brain, which is in marked contrast to the tissue distribution of Rab8. The Rab8b cDNA was modified to introduce a c-myc epitope tag at the extreme N terminus of the protein, and transient transfection studies were performed to analyse the intracellular localization of Rab8b by confocal microscopy. Transient expression of the c-myc/Rab8b fusion protein in both PC12 and RBL.2H3 cells shows staining of both the plasma membrane and ill-defined vesicular structures, and in the case of RBL.2H3 cells appears to induce striking outgrowths of the plasma membrane.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				S. L. Hall, S. Hester, J. L. Griffin, K. S. Lilley, and A. P. Jackson The Organelle Proteome of the DT40 Lymphocyte Cell Line Mol. Cell. Proteomics, June 1, 2009; 8(6): 1295 - 1305. [Abstract] [Full Text] [PDF]

				K. F. Leung, R. Baron, B. R. Ali, A. I. Magee, and M. C. Seabra Rab GTPases Containing a CAAX Motif Are Processed Post-geranylgeranylation by Proteolysis and Methylation J. Biol. Chem., January 12, 2007; 282(2): 1487 - 1497. [Abstract] [Full Text] [PDF]

				K. Hattula, J. Furuhjelm, J. Tikkanen, K. Tanhuanpaa, P. Laakkonen, and J. Peranen Characterization of the Rab8-specific membrane traffic route linked to protrusion formation J. Cell Sci., December 1, 2006; 119(23): 4866 - 4877. [Abstract] [Full Text] [PDF]

				D. D. Mruk and C. Y. Cheng Sertoli-Sertoli and Sertoli-Germ Cell Interactions and Their Significance in Germ Cell Movement in the Seminiferous Epithelium during Spermatogenesis Endocr. Rev., October 1, 2004; 25(5): 747 - 806. [Abstract] [Full Text] [PDF]

				A. S. N. Lau and D. D. Mruk Rab8B GTPase and Junction Dynamics in the Testis Endocrinology, April 1, 2003; 144(4): 1549 - 1563. [Abstract] [Full Text] [PDF]

				K. Hattula, J. Furuhjelm, A. Arffman, and J. Peranen A Rab8-specific GDP/GTP Exchange Factor Is Involved in Actin Remodeling and Polarized Membrane Transport Mol. Biol. Cell, September 1, 2002; 13(9): 3268 - 3280. [Abstract] [Full Text] [PDF]

				F. Opdam, A Echard, H. Croes, J. van den Hurk, R. van de Vorstenbosch, L. Ginsel, B Goud, and J. Fransen The small GTPase Rab6B, a novel Rab6 subfamily member, is cell-type specifically expressed and localised to the Golgi apparatus J. Cell Sci., January 8, 2000; 113(15): 2725 - 2735. [Abstract] [PDF]

				J Rowe, N Corradi, M. Malosio, E Taverna, P Halban, J Meldolesi, and P Rosa Blockade of membrane transport and disassembly of the Golgi complex by expression of syntaxin 1A in neurosecretion-incompetent cells: prevention by rbSEC1 J. Cell Sci., January 6, 1999; 112(12): 1865 - 1877. [Abstract] [PDF]

				S. Chen, M. C. Liang, J. N. Chia, J. K. Ngsee, and A. E. Ting Rab8b and Its Interacting Partner TRIP8b Are Involved in Regulated Secretion in AtT20 Cells J. Biol. Chem., April 13, 2001; 276(16): 13209 - 13216. [Abstract] [Full Text] [PDF]