|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 109, Issue 6 1325-1334, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
A Tousson, CM Fuller and DJ Benos
Department of Cell Biology, University of Alabama at Birmingham 35294, USA.
Previous studies from this laboratory have demonstrated that chloride transport induced by forskolin, but not ionomycin, in T84 cells is highly dependent on an intact microtubular network. Using an antibody raised against a region of the R domain of CFTR, we now show by indirect immunofluorescence that forskolin causes relocation of CFTR to the apical domain of T84 cells. T84 cells grown on transparent filters were incubated with agonists and/or cytoskeletal inhibitors prior to fixation, permeabilization, and staining with the antibody. A 30 second stimulation with forskolin (10 microM) caused a twofold increase in relative fluorescence intensity at the apical surface. In contrast, a 30 second exposure to ionomycin (2 microM), had no effect on the distribution of CFTR-related fluorescence. Incubation of the cells with nocodazole (33 microM), a microtubule disrupting agent, prevented the forskolin-induced rise in CFTR fluorescence at the apical surface. However, incubation of the cells with cytochalasin D, an actin inhibitor, was without effect on forskolin-related re-distribution of CFTR-associated fluorescence. In double label experiments using antibodies against both beta-tubulin and actin, CFTR-related fluorescence was found to co-localize with the microtubule network, but not with actin filaments. These observations are consistent with the microtubule-dependent acute recruitment of CFTR to the apical plasma membrane of T84 cells in response to elevations in intracellular cAMP.
This article has been cited by other articles:
|
|
 |
|
  H. F. Bao, L. Liu, J. Self, B. J. Duke, R. Ueno, and D. C. Eaton A synthetic prostone activates apical chloride channels in A6 epithelial cells Am J Physiol Gastrointest Liver Physiol, August 1, 2008; 295(2): G234 - G251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. S. Nicolas, K.-H. Park, A. El Harchi, J. Camonis, R. S. Kass, D. Escande, J. Merot, G. Loussouarn, F. Le Bouffant, and I. Baro IKs response to protein kinase A-dependent KCNQ1 phosphorylation requires direct interaction with microtubules Cardiovasc Res, August 1, 2008; 79(3): 427 - 435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Auerbach and C. M. Liedtke Role of the scaffold protein RACK1 in apical expression of CFTR Am J Physiol Cell Physiol, July 1, 2007; 293(1): C294 - C304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Fu and B. D. Roufogalis Actin disruption inhibits endosomal traffic of P-glycoprotein-EGFP and resistance to daunorubicin accumulation Am J Physiol Cell Physiol, April 1, 2007; 292(4): C1543 - C1552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Broughman, L. Sun, S. Umar, J. Scott, J. H. Sellin, and A. P. Morris Chronic PKC-beta activation in HT-29 Cl.19a colonocytes prevents cAMP-mediated ion secretion by inhibiting apical membrane current generation Am J Physiol Gastrointest Liver Physiol, August 1, 2006; 291(2): G318 - G330. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Guerra, T. Fanelli, M. Favia, S. M. Riccardi, G. Busco, R. A. Cardone, S. Carrabino, E. J. Weinman, S. J. Reshkin, M. Conese, et al. Na+/H+ Exchanger Regulatory Factor Isoform 1 Overexpression Modulates Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Expression and Activity in Human Airway 16HBE14o- Cells and Rescues {Delta}F508 CFTR Functional Expression in Cystic Fibrosis Cells J. Biol. Chem., December 9, 2005; 280(49): 40925 - 40933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Skinn and W. K. MacNaughton Nitric oxide inhibits cAMP-dependent CFTR trafficking in intestinal epithelial cells Am J Physiol Gastrointest Liver Physiol, October 1, 2005; 289(4): G739 - G744. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cuppoletti, D. H. Malinowska, K. P. Tewari, Q.-j. Li, A. M. Sherry, M. L. Patchen, and R. Ueno SPI-0211 activates T84 cell chloride transport and recombinant human ClC-2 chloride currents Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1173 - C1183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Kaspar, D. D. H. Thomas, W. B. Taft, E. Takeshita, N. Weng, and G. E. Groblewski CaM kinase II regulation of CRHSP-28 phosphorylation in cultured mucosal T84 cells Am J Physiol Gastrointest Liver Physiol, December 1, 2003; 285(6): G1300 - G1309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Walker, H. B. Jijon, T. Churchill, M. Kulka, and K. L. Madsen Activation of AMP-activated protein kinase reduces cAMP-mediated epithelial chloride secretion Am J Physiol Gastrointest Liver Physiol, November 1, 2003; 285(5): G850 - G860. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Dalby-Payne, E. V. O'Loughlin, and P. Gunning Polarization of Specific Tropomyosin Isoforms in Gastrointestinal Epithelial Cells and Their Impact on CFTR at the Apical Surface Mol. Biol. Cell, November 1, 2003; 14(11): 4365 - 4375. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. A. Assef, A. E. Damiano, E. Zotta, C. Ibarra, and B. A. Kotsias CFTR in K562 human leukemic cells Am J Physiol Cell Physiol, August 1, 2003; 285(2): C480 - C488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Bertrand and R. A. Frizzell The role of regulated CFTR trafficking in epithelial secretion Am J Physiol Cell Physiol, July 1, 2003; 285(1): C1 - C18. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Ameen, C. Marino, and P. J. I. Salas cAMP-dependent exocytosis and vesicle traffic regulate CFTR and fluid transport in rat jejunum in vivo Am J Physiol Cell Physiol, February 1, 2003; 284(2): C429 - C438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cheng, B. D. Moyer, M. Milewski, J. Loffing, M. Ikeda, J. E. Mickle, G. R. Cutting, M. Li, B. A. Stanton, and W. B. Guggino A Golgi-associated PDZ Domain Protein Modulates Cystic Fibrosis Transmembrane Regulator Plasma Membrane Expression J. Biol. Chem., January 25, 2002; 277(5): 3520 - 3529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Weixel and N. A. Bradbury {micro}2 Binding Directs the Cystic Fibrosis Transmembrane Conductance Regulator to the Clathrin-mediated Endocytic Pathway J. Biol. Chem., November 30, 2001; 276(49): 46251 - 46259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Buresi, E. Schleihauf, N. Vergnolle, A. Buret, J. L. Wallace, M. D. Hollenberg, and W. K. MacNaughton Protease-activated receptor-1 stimulates Ca2+-dependent Cl{-} secretion in human intestinal epithelial cells Am J Physiol Gastrointest Liver Physiol, August 1, 2001; 281(2): G323 - G332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. V. Caler, R. E. Morty, B. A. Burleigh, and N. W. Andrews Dual Role of Signaling Pathways Leading to Ca2+ and Cyclic AMP Elevation in Host Cell Invasion by Trypanosoma cruzi Infect. Immun., December 1, 2000; 68(12): 6602 - 6610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Dickson, T. D. Conner, and T. W. Ecay Inhibition of phosphatidylinositol 3-kinase does not alter forskolin-stimulated Cl- secretion by T84 cells Am J Physiol Cell Physiol, May 1, 2000; 278(5): C865 - C872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Gomez, B. G. Kerfant, and G. Vassort Microtubule Disruption Modulates Ca2+ Signaling in Rat Cardiac Myocytes Circ. Res., January 7, 2000; 86(1): 30 - 36. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rodriguez, I. Martinez, A. Chung, C. H. Berlot, and N. W. Andrews cAMP Regulates Ca2+-dependent Exocytosis of Lysosomes and Lysosome-mediated Cell Invasion by Trypanosomes J. Biol. Chem., June 11, 1999; 274(24): 16754 - 16759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Navarro-Garcia, C. Sears, C. Eslava, A. Cravioto, and J. P. Nataro Cytoskeletal Effects Induced by Pet, the Serine Protease Enterotoxin of Enteroaggregative Escherichia coli Infect. Immun., May 1, 1999; 67(5): 2184 - 2192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Aller, I. D. Lombardo, S. Bhanot, and J. N. Forrest Jr. Cloning, characterization, and functional expression of a CNP receptor regulating CFTR in the shark rectal gland Am J Physiol Cell Physiol, February 1, 1999; 276(2): C442 - C449. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Ameen, B Martensson, L Bourguinon, C Marino, J Isenberg, and G. McLaughlin CFTR channel insertion to the apical surface in rat duodenal villus epithelial cells is upregulated by VIP in vivo J. Cell Sci., January 3, 1999; 112(6): 887 - 894. [Abstract] [PDF]
|
|
|
|
 |
|
 J. M. PILEWSKI and R. A. FRIZZELL Role of CFTR in Airway Disease Physiol Rev, January 1, 1999; 79(1): 215 - 255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. B. Guggino Focus on "Exocytosis is not involved in activation of Cl- secretion via CFTR in Calu-3 airway epithelial cells" Am J Physiol Cell Physiol, October 1, 1998; 275(4): C911 - C912. [Full Text] [PDF]
|
|
|
|
|
|
J. Loffing, B. D. Moyer, D. McCoy, and B. A. Stanton Exocytosis is not involved in activation of Cl- secretion via CFTR in Calu-3 airway epithelial cells Am J Physiol Cell Physiol, October 1, 1998; 275(4): C913 - C920. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Moyer, J. Loffing, E. M. Schwiebert, D. Loffing-Cueni, P. A. Halpin, K. H. Karlson, I. I. Ismailov, W. B. Guggino, G. M. Langford, and B. A. Stanton Membrane Trafficking of the Cystic Fibrosis Gene Product, Cystic Fibrosis Transmembrane Conductance Regulator, Tagged with Green Fluorescent Protein in Madin-Darby Canine Kidney Cells J. Biol. Chem., August 21, 1998; 273(34): 21759 - 21768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C. Calhoun, L. A. Lapierre, C. S. Chew, and J. R. Goldenring Rab11a redistributes to apical secretory canaliculus during stimulation of gastric parietal cells Am J Physiol Cell Physiol, July 1, 1998; 275(1): C163 - C170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Morris, A. Tousson, D. J. Benos, and J. A. Schafer Microtubule disruption inhibits AVT-stimulated Cl- secretion but not Na+ reabsorption in A6 cells Am J Physiol Renal Physiol, February 1, 1998; 274(2): F300 - F314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-L. Ji, M. L. Chalfant, B. Jovov, J. P. Lockhart, S. B. Parker, C. M. Fuller, B. A. Stanton, and D. J. Benos The Cytosolic Termini of the beta - and gamma -ENaC Subunits Are Involved in the Functional Interactions between Cystic Fibrosis Transmembrane Conductance Regulator and Epithelial Sodium Channel J. Biol. Chem., September 1, 2000; 275(36): 27947 - 27956. [Abstract] [Full Text] [PDF]
|
|
