|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 109, Issue 6 1449-1457, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
A Dickmanns, FR Bischoff, C Marshallsay, R Luhrmann, H Ponstingl and E Fanning
Department of Molecular Biology, Vanderbilt University, Nashville, TN 37235, USA.
The nuclear protein regulator of chromosome condensation 1 (RCC1) stimulates guanine nucleotide exchange on a protein, Ran, that is required for nuclear protein import. In the present report, we confirm that RCC1 is also required for nuclear protein import in tsBN2 hamster cells in vivo. The thermolability of nuclear protein import in tsBN2 cells was suppressed by microinjection of purified Ran-GTP into the cytoplasm, but Ran-GDP also relieved the import deficiency, suggesting either that both forms of Ran are active in import in vivo or that tsBN2 cells at restrictive temperature retain a mechanism to convert Ran-GDP to Ran-GTP. To distinguish between these possibilities, nuclear protein import in tsBN2 cells was tested in the presence of Ran mutants, one deficient in GTP hydrolysis (RanQ69L), and one with weak binding to GDP and little or no binding to GTP (RanT24N). Microinjection of the mutant RanQ69L inhibited import in vivo in either the GTP- or GDP-bound form at both the permissive and nonpermissive temperatures. RanT24N-GDP inhibited import in vivo at the permissive temperature and failed to stimulate nuclear protein import at the nonpermissive temperature. The implications of these results for the roles of RCC1 and Ran in nuclear protein import in vivo are discussed.
This article has been cited by other articles:
|
|
 |
|
  S. A. Adam, K. Sengupta, and R. D. Goldman Regulation of Nuclear Lamin Polymerization by Importin {alpha} J. Biol. Chem., March 28, 2008; 283(13): 8462 - 8468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Harreman, M. R. Hodel, P. Fanara, A. E. Hodel, and A. H. Corbett The Auto-inhibitory Function of Importin alpha Is Essential in Vivo J. Biol. Chem., February 14, 2003; 278(8): 5854 - 5863. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. J. Watkins, A. Dickmanns, and R. Luhrmann Conserved Stem II of the Box C/D Motif Is Essential for Nucleolar Localization and Is Required, Along with the 15.5K Protein, for the Hierarchical Assembly of the Box C/D snoRNP Mol. Cell. Biol., December 1, 2002; 22(23): 8342 - 8352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Karsies, T. Merkle, B. Szurek, U. Bonas, T. Hohn, and D. Leclerc Regulated nuclear targeting of cauliflower mosaic virus J. Gen. Virol., June 1, 2002; 83(7): 1783 - 1790. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Huber, A. Dickmanns, and R. Luhrmann The importin-{beta} binding domain of snurportin1 is responsible for the Ran- and energy-independent nuclear import of spliceosomal U snRNPs in vitro J. Cell Biol., February 4, 2002; 156(3): 467 - 479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Kehlenbach, A. Dickmanns, A. Kehlenbach, T. Guan, and L. Gerace A Role for RanBP1 in the Release of CRM1 from the Nuclear Pore Complex in a Terminal Step of Nuclear Export J. Cell Biol., May 17, 1999; 145(4): 645 - 657. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Yokoya, N. Imamoto, T. Tachibana, and Y. Yoneda beta -Catenin Can Be Transported into the Nucleus in a Ran-unassisted Manner Mol. Biol. Cell, April 1, 1999; 10(4): 1119 - 1131. [Abstract] [Full Text]
|
|
|
|
|
|
R. H. Kehlenbach, A. Dickmanns, and L. Gerace Nucleocytoplasmic Shuttling Factors Including Ran and CRM1 Mediate Nuclear Export of NFAT In Vitro J. Cell Biol., May 18, 1998; 141(4): 863 - 874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kose, N. Imamoto, T. Tachibana, T. Shimamoto, and Y. Yoneda Ran-unassisted Nuclear Migration of a 97-kD Component of Nuclear Pore-targeting Complex J. Cell Biol., November 17, 1997; 139(4): 841 - 849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Boche and E. Fanning Nucleocytoplasmic Recycling of the Nuclear Localization Signal Receptor alpha Subunit In Vivo Is Dependent on a Nuclear Export Signal, Energy, and RCC1 J. Cell Biol., October 20, 1997; 139(2): 313 - 325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Nishijima, H. Nishitani, T. Seki, and T. Nishimoto A Dual-Specificity Phosphatase Cdc25B Is an Unstable Protein and Triggers p34cdc2/Cyclin B Activation in Hamster BHK21 Cells Arrested with Hydroxyurea J. Cell Biol., September 8, 1997; 138(5): 1105 - 1116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sekimoto, K. Nakajima, T. Tachibana, T. Hirano, and Y. Yoneda Interferon-gamma -dependent Nuclear Import of Stat1 Is Mediated by the GTPase Activity of Ran/TC4 J. Biol. Chem., December 6, 1996; 271(49): 31017 - 31020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Huber, A. Dickmanns, and R. Luhrmann The importin-{beta} binding domain of snurportin1 is responsible for the Ran- and energy-independent nuclear import of spliceosomal U snRNPs in vitro J. Cell Biol., February 4, 2002; 156(3): 467 - 479. [Abstract] [Full Text] [PDF]
|
|
