spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hempel, K.
Right arrow Articles by Stratling, W. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hempel, K.
Right arrow Articles by Stratling, W. H.

Journal of Cell Science, Vol 109, Issue 6 1459-1469, Copyright © 1996 by Company of Biologists

JOURNAL ARTICLES

The chicken lysozyme gene 5' MAR and the Drosophila histone SAR are electroelutable from encapsulated and digested nuclei

K Hempel and WH Stratling
Institut fur Physiologische Chemie, Universitats-Krankenhaus Eppendorf, Hamburg, FR Germany.

Cultured chicken cells were encapsulated in agarose microbeads, lysed in a near-physiological buffer and resulting encapsulated nuclei were digested with a restriction enzyme and electroeluted. After removal of approximately 97% of the chromatin, the nuclear lamina, residual nucleoli and an internal nuclear network remained. The majority of nascent RNA was also recovered in digested and electroeluted nuclei. Surprisingly, however, the chicken lysozyme gene 5' MAR was quantitatively electroeluted from digested nuclei of expressing and non-expressing cells, as well as the promoter region and the coding sequence. When encapsulated nuclei were digested partially, the proportion of elutable 5' MAR chromatin was comparable to that of elutable bulk chromatin. Furthermore, after digestion of encapsulated nuclei from Drosophila Kc cells, the histone SAR was electroeluted to the same extent as bulk chromatin. We conclude that the lysozyme gene 5' MAR and the histone SAR are not permanently attached to a nuclear matrix or scaffold.


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
O. V. Iarovaia, A. Bystritskiy, D. Ravcheev, R. Hancock, and S. V. Razin
Visualization of individual DNA loops and a map of loop domains in the human dystrophin gene
Nucleic Acids Res., April 15, 2004; 32(7): 2079 - 2086.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. H. Q. Heng, S. Goetze, C. J. Ye, G. Liu, J. B. Stevens, S. W. Bremer, S. M. Wykes, J. Bode, and S. A. Krawetz
Chromatin loops are selectively anchored using scaffold/matrix-attachment regions
J. Cell Sci., March 1, 2004; 117(7): 999 - 1008.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Ortega and M. DePamphilis
Nucleoskeleton and initiation of DNA replication in metazoan cells
J. Cell Sci., June 14, 1999; 111(24): 3663 - 3673.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
C. Bonifer, U. Jagle, and M. C. Huber
The Chicken Lysozyme Locus as a Paradigm for the Complex Developmental Regulation of Eukaryotic Gene Loci
J. Biol. Chem., October 17, 1997; 272(42): 26075 - 26078.
[Full Text] [PDF]


© The Company of Biologists Ltd 1996