|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 109, Issue 6 1605-1613, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
J Xu, M Liu, J Liu, I Caniggia and M Post
Medical Research Council Group in Lung Development, Hospital for Sick Children Research Institute, University of Toronto, Canada.
We have previously shown that an intermittent strain regimen, which simulates fetal breathing movements, enhanced mixed fetal rat lung cell proliferation in organotypic culture. As glycosaminoglycans (GAGs) and proteoglycans (PGs) may modulate growth factor activities, we investigated the effect of intermittent strain on the formation and secretion of GAGs and PGs. Mechanical strain increased the incorporation of [3H]glucosamine and 35SO4 into GAGs and promoted the release of GAGs into the medium. The composition of the individual GAG molecules was not altered by strain. Mixed fetal lung cells subjected to strain secreted more [35S]biglycan into the medium than static controls but biglycan mRNA expression was not significantly altered. As mechanical strain primarily affected the secretion of GAGs and PGs, we then investigated which secretory pathways were stimulated by strain. Fetal lung cells secreted GAGs mainly through a constitutive (basal) pathway which was stimulated by strain. In contrast to static cultures, strain-induced constitutive secretion was partially blocked by the cytoskeletal disruptors colchicine and cytochalasin B, but not by the small G-protein inhibitors N-acetyl-S-farnesyl-L-cysteine and perillic acid. This result suggests that strain-induced constitutive export of GAGs depends on the functional integrity of the cytoskeleton. Strain also triggered the regulated secretion of GAGs. The strain-induced regulatory pathway in fetal lung cells was blocked by ionomycin, BAPTA/AM and gadolinium, suggesting that strain stimulated the regulatory pathway by inducing a rapid calcium influx via a stretch-activated ion channel. We conclude that mechanical strain of mixed fetal lung cells stimulates GAG and PG exocytosis via activation of both the regulated and constitutive pathways.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  C. F. Andrade, A. P. Wong, T. K. Waddell, S. Keshavjee, and M. Liu Cell-based tissue engineering for lung regeneration Am J Physiol Lung Cell Mol Physiol, February 1, 2007; 292(2): L510 - L518. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Yan, D. J. Bellotto, D. M. Dane, R. G. Elmore, R. L. Johnson Jr., A. S. Estrera, and C. C. W. Hsia Lack of response to all-trans retinoic acid supplementation in adult dogs following left pneumonectomy J Appl Physiol, November 1, 2005; 99(5): 1681 - 1688. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Han, M. Lodyga, and M. Liu Ventilator-induced Lung Injury: Role of Protein-Protein Interaction in Mechanosensation Proceedings of the ATS, October 1, 2005; 2(3): 181 - 187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-J. Bai, A. P. Spicer, M. M. Mascarenhas, L. Yu, C. D. Ochoa, H. G. Garg, and D. A. Quinn The Role of Hyaluronan Synthase 3 in Ventilator-induced Lung Injury Am. J. Respir. Crit. Care Med., July 1, 2005; 172(1): 92 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. W. Hsia Signals and mechanisms of compensatory lung growth J Appl Physiol, November 1, 2004; 97(5): 1992 - 1998. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Mascarenhas, R. M. Day, C. D. Ochoa, W.-I. Choi, L. Yu, B. Ouyang, H. G. Garg, C. A. Hales, and D. A. Quinn Low Molecular Weight Hyaluronan from Stretched Lung Enhances Interleukin-8 Expression Am. J. Respir. Cell Mol. Biol., January 1, 2004; 30(1): 51 - 60. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Ahmed, E. Oie, L. E. Vinge, A. Yndestad, G. O. Andersen, Y. Andersson, T. Attramadal, and H. Attramadal Induction of myocardial biglycan in heart failure in rats--an extracellular matrix component targeted by AT1 receptor antagonism Cardiovasc Res, December 1, 2003; 60(3): 557 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lodyga, X.-H. Bai, E. Mourgeon, B. Han, S. Keshavjee, and M. Liu Molecular cloning of actin filament-associated protein: a putative adaptor in stretch-induced Src activation Am J Physiol Lung Cell Mol Physiol, August 1, 2002; 283(2): L265 - L274. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. T. Truschel, E. Wang, W. G. Ruiz, S.-M. Leung, R. Rojas, J. Lavelle, M. Zeidel, D. Stoffer, and G. Apodaca Stretch-regulated Exocytosis/Endocytosis in Bladder Umbrella Cells Mol. Biol. Cell, March 1, 2002; 13(3): 830 - 846. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Apodaca Modulation of membrane traffic by mechanical stimuli Am J Physiol Renal Physiol, February 1, 2002; 282(2): F179 - F190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Al-Jamal and M. S. Ludwig Changes in proteoglycans and lung tissue mechanics during excessive mechanical ventilation in rats Am J Physiol Lung Cell Mol Physiol, November 1, 2001; 281(5): L1078 - L1087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. W. Hsia, E. Y. Wu, E. Wagner, and E. R. Weibel Preventing mediastinal shift after pneumonectomy impairs regenerative alveolar tissue growth Am J Physiol Lung Cell Mol Physiol, November 1, 2001; 281(5): L1279 - L1287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Haley, M. E. Sunday, R. Osathanondh, J. Du, C. Vathanaprida, V. V. Karpitsky, J. E. Krause, and C. M. Lilly Developmental expression of neurokinin A and functional neurokinin-2 receptors in lung Am J Physiol Lung Cell Mol Physiol, June 1, 2001; 280(6): L1348 - L1358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Liu and M. Post Cellular Responses to Mechanical Stress: Invited Review: Mechanochemical signal transduction in the fetal lung J Appl Physiol, November 1, 2000; 89(5): 2078 - 2084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Mourgeon, N. Isowa, S. Keshavjee, X. Zhang, A. S. Slutsky, and M. Liu Mechanical stretch stimulates macrophage inflammatory protein-2 secretion from fetal rat lung cells Am J Physiol Lung Cell Mol Physiol, October 1, 2000; 279(4): L699 - L706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nakamura, M. Liu, E. Mourgeon, A. Slutsky, and M. Post Mechanical strain and dexamethasone selectively increase surfactant protein C and tropoelastin gene expression Am J Physiol Lung Cell Mol Physiol, May 1, 2000; 278(5): L974 - L980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Liu, A. K. Tanswell, and M. Post Mechanical force-induced signal transduction in lung cells Am J Physiol Lung Cell Mol Physiol, October 1, 1999; 277(4): L667 - L683. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Mourgeon, J. Xu, A. K. Tanswell, M. Liu, and M. Post Mechanical strain-induced posttranscriptional regulation of fibronectin production in fetal lung cells Am J Physiol Lung Cell Mol Physiol, July 1, 1999; 277(1): L142 - L149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Xu, M. Liu, and M. Post Differential regulation of extracellular matrix molecules by mechanical strain of fetal lung cells Am J Physiol Lung Cell Mol Physiol, May 1, 1999; 276(5): L728 - L735. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. C. Chan, B. Baranowski, L. Berry, M. Liu, B. Rafii, M. Post, H. O'Brodovich, P. Monagle, and M. Andrew Influence of Mechanical Stretch on Thrombin Regulation by Fetal Mixed Lung Cells Am. J. Respir. Cell Mol. Biol., September 1, 1998; 19(3): 419 - 425. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. Maroto and O. P. Hamill Brefeldin A Block of Integrin-dependent Mechanosensitive ATP Release from Xenopus Oocytes Reveals a Novel Mechanism of Mechanotransduction J. Biol. Chem., June 22, 2001; 276(26): 23867 - 23872. [Abstract] [Full Text] [PDF]
|
|
