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Journal of Cell Science, Vol 109, Issue 7 1759-1764, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
F Depoortere, JE Dumont and PP Roger
Institute of Interdisciplinary Research, Universite Libre de Bruxelles, Belgium.
In different systems, cAMP either blocks or promotes cell cycle progression in mid to late G1 phase. Dog thyroid epithelial cells in primary culture constitute a model of positive control of DNA synthesis initiation and G0-S pre-replicative phase progression by cyclic AMP (cAMP) as a second messenger for thyrotropin (TSH). We report here that TSH markedly increases the expression of p27kip1, the inhibitor of the cell cycle and cyclin-dependent kinases. This effect was prevented by the concomitant administration of the cAMP-independent mitogens, epidermal growth factor (EGF)+serum. EGF+serum also slightly inhibited the weak basal accumulation of p27kip1. Nevertheless, in the case of stimulation by TSH alone, the cAMP-dependent cell cycle progression was fully compatible with the enhanced expression of p27kip1. This observation is paradoxical since a decrease of p27kip1 is generally associated with growth stimulation in other systems, and since a similar cAMP-dependent increase of p27kip1 in macrophages has been found responsible for mid-G1 cell cycle arrest. The opposite regulation of p27kip1 in response to TSH or EGF+serum in dog thyroid epithelial cells suggests a major difference at mid to late G1 stages between cAMP-dependent and cAMP-independent mitogenic pathways.
This article has been cited by other articles:
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  A. S. Rocha, S. Paternot, K. Coulonval, J. E. Dumont, P. Soares, and P. P. Roger Cyclic AMP Inhibits the Proliferation of Thyroid Carcinoma Cell Lines through Regulation of CDK4 Phosphorylation Mol. Biol. Cell, November 1, 2008; 19(11): 4814 - 4825. [Abstract] [Full Text] [PDF]
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 S. Paternot, J. E. Dumont, and P. P. Roger Differential Utilization of Cyclin D1 and Cyclin D3 in the Distinct Mitogenic Stimulations by Growth Factors and TSH of Human Thyrocytes in Primary Culture Mol. Endocrinol., December 1, 2006; 20(12): 3279 - 3292. [Abstract] [Full Text] [PDF]
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 L. Bockstaele, H. Kooken, F. Libert, S. Paternot, J. E. Dumont, Y. de Launoit, P. P. Roger, and K. Coulonval Regulated Activating Thr172 Phosphorylation of Cyclin-Dependent Kinase 4(CDK4): Its Relationship with Cyclins and CDK "Inhibitors". Mol. Cell. Biol., July 1, 2006; 26(13): 5070 - 5085. [Abstract] [Full Text] [PDF]
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A. E. Lewis, A. J. Fikaris, G. V. Prendergast, and J. L. Meinkoth Thyrotropin and Serum Regulate Thyroid Cell Proliferation through Differential Effects on p27 Expression and Localization Mol. Endocrinol., September 1, 2004; 18(9): 2321 - 2332. [Abstract] [Full Text] [PDF]
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 S. Paternot, K. Coulonval, J. E. Dumont, and P. P. Roger Cyclic AMP-dependent Phosphorylation of Cyclin D3-bound CDK4 Determines the Passage through the Cell Cycle Restriction Point in Thyroid Epithelial Cells J. Biol. Chem., July 11, 2003; 278(29): 26533 - 26540. [Abstract] [Full Text] [PDF]
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 T. Kimura, A. Van Keymeulen, J. Golstein, A. Fusco, J. E. Dumont, and P. P. Roger Regulation of Thyroid Cell Proliferation by TSH and Other Factors: A Critical Evaluation of in Vitro Models Endocr. Rev., October 1, 2001; 22(5): 631 - 656. [Abstract] [Full Text] [PDF]
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 A. Van Keymeulen, S. Deleu, J. Bartek, J. E. Dumont, and P. P. Roger Respective Roles of Carbamylcholine and Cyclic Adenosine Monophosphate in Their Synergistic Regulation of Cell Cycle in Thyroid Primary Cultures Endocrinology, March 1, 2001; 142(3): 1251 - 1259. [Abstract] [Full Text] [PDF]
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R. H. Weiss, A. Joo, and C. Randour p21Waf1/Cip1 Is an Assembly Factor Required for Platelet-derived Growth Factor-induced Vascular Smooth Muscle Cell Proliferation J. Biol. Chem., March 31, 2000; 275(14): 10285 - 10290. [Abstract] [Full Text] [PDF]
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F. Depoortere, I. Pirson, J. Bartek, J. E. Dumont, and P. P. Roger Transforming Growth Factor beta 1 Selectively Inhibits the Cyclic AMP-dependent Proliferation of Primary Thyroid Epithelial Cells by Preventing the Association of Cyclin D3-cdk4 with Nuclear p27kip1 Mol. Biol. Cell, March 1, 2000; 11(3): 1061 - 1076. [Abstract] [Full Text]
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 S. Chevalier and R.A. Roberts G1-arrested FaO cells re-enter the cell cycle upon stimulation with the rodent non-genotoxic hepatocarcinogen nafenopin Carcinogenesis, July 1, 1999; 20(7): 1209 - 1213. [Abstract] [Full Text] [PDF]
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 M. Derwahl, M. Broecker, and Z. Kraiem Thyrotropin May Not Be the Dominant Growth Factor in Benign and Malignant Thyroid Tumors J. Clin. Endocrinol. Metab., March 1, 1999; 84(3): 829 - 834. [Full Text]
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 F. Depoortere, A. Van Keymeulen, J. Lukas, S. Costagliola, J. Bartkova, J. E. Dumont, J. Bartek, P. P. Roger, and S. Dremier A Requirement for Cyclin D3-Cyclin-dependent Kinase (cdk)-4 Assembly in the Cyclic Adenosine Monophosphate-dependent Proliferation of Thyrocytes J. Cell Biol., March 23, 1998; 140(6): 1427 - 1439. [Abstract] [Full Text] [PDF]
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