|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 109, Issue 7 1875-1889, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
A Zuk and KS Matlin
Renal Unit, Massachusetts General Hospital, Charlestown, USA.
A number of epithelia form tubulocysts in vitro when overlaid with type I collagen gel. Because collagen receptors are generally believed to be expressed on the basolateral domain, the mechanism by which collagen elicits this morphogenetic response from the apical surface is unclear. To investigate the role of beta 1 integrins, the major receptor family for collagen, in this process, we overlaid polarized monolayers of MDCK II cells grown on permeable supports with type I collagen gel and correlated integrin polarity with the polarity of other apical and basolateral membrane markers during tubulocyst formation. Polarized monolayers of one clone of MDCK II cells, referred to as Heidelberg MDCK, initially respond to collagen overlay by stratifying; within 48 hours, lumena develop between the cell layers giving rise to tubulocysts. Tight junctions remain intact during tubulocyst formation because transepithelial electrical resistance does not significantly change. Major alterations are observed, however, in the expression and localization of apical and basolateral membrane markers. beta 1 integrins are necessary for tubulocyst morphogenesis because a function-blocking antibody administered to the apical pole of the cells completely inhibits the formation of these structures. To determine how apical-cell collagen interactions elicit tubulocyst formation, we examined whether beta 1 integrins are mobilized to apical plasma membranes in response to collagen overlay. We found that in the absence of collagen, polarized monolayers of Heidelberg MDCK cells endogenously express on apical plasma membranes a small pool of the beta 1 family, including alpha 2 beta 1 and alpha 3 beta 1. Collagen overlay does not mobilize additional beta 1 integrins to apical domains. If beta 1 integrins are not already apically expressed, as in the C6 MDCK cell line (Schoenenberger et al. (1994) J. Cell Biol. 107, 527-541), beta 1 integrins are not directed apically and tubulocysts do not develop in response to collagen. Thus, interaction of beta 1 integrin pre-existing on apical plasma membranes of polarized epithelia with type I collagen gel is the mechanism by which apical application of collagen elicits the formation of tubulocysts. Depolarized integrins on apical plasma membranes of polarized epithelia may be relevant to the pathogenesis of disease and injury.
This article has been cited by other articles:
|
|
 |
|
  J. Friedrichs, A. Manninen, D. J. Muller, and J. Helenius Galectin-3 Regulates Integrin {alpha}2{beta}1-mediated Adhesion to Collagen-I and -IV J. Biol. Chem., November 21, 2008; 283(47): 32264 - 32272. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Battini, E. Fedorova, S. Macip, X. Li, P. D. Wilson, and G. L. Gusella Stable Knockdown of Polycystin-1 Confers Integrin-{alpha}2beta1-Mediated Anoikis Resistance J. Am. Soc. Nephrol., November 1, 2006; 17(11): 3049 - 3058. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Eisen, S. Walid, D. R. Ratcliffe, and G. K. Ojakian Regulation of epithelial tubule formation by Rho family GTPases Am J Physiol Cell Physiol, May 1, 2006; 290(5): C1297 - C1309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Yu, A. Datta, P. Leroy, L. E. O'Brien, G. Mak, T.-S. Jou, K. S. Matlin, K. E. Mostov, and M. M.P. Zegers {beta}1-Integrin Orients Epithelial Polarity via Rac1 and Laminin Mol. Biol. Cell, February 1, 2005; 16(2): 433 - 445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Eisen, D. R. Ratcliffe, and G. K. Ojakian Modulation of epithelial tubule formation by Rho kinase Am J Physiol Cell Physiol, April 1, 2004; 286(4): C857 - C866. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Ojakian, D. Ratcliffe, and R Schwimmer Integrin regulation of cell-cell adhesion during epithelial tubule formation J. Cell Sci., January 3, 2001; 114(5): 941 - 952. [Abstract] [PDF]
|
|
|
|
 |
|
 C. Coraux, J.-M. Zahm, E. Puchelle, and D. Gaillard beta 1-Integrins are involved in migration of human fetal tracheal epithelial cells and tubular morphogenesis Am J Physiol Lung Cell Mol Physiol, August 1, 2000; 279(2): L224 - L234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. YEAMAN, K. K. GRINDSTAFF, and W. J. NELSON New Perspectives on Mechanisms Involved in Generating Epithelial Cell Polarity Physiol Rev, January 1, 1999; 79(1): 73 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gut, M. S. Balda, and K. Matter The Cytoplasmic Domains of a beta 1 Integrin Mediate Polarization in Madin-Darby Canine Kidney Cells by Selective Basolateral Stabilization J. Biol. Chem., November 6, 1998; 273(45): 29381 - 29388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zuk, J. V. Bonventre, D. Brown, and K. S. Matlin Polarity, integrin, and extracellular matrix dynamics in the postischemic rat kidney Am J Physiol Cell Physiol, September 1, 1998; 275(3): C711 - C731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ojakian, W. Nelson, and K. Beck Mechanisms for de novo biogenesis of an apical membrane compartment in groups of simple epithelial cells surrounded by extracellular matrix J. Cell Sci., January 11, 1997; 110(22): 2781 - 2794. [Abstract] [PDF]
|
|
