|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 109, Issue 7 1891-1898, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
PS Criswell, LE Ostrowski and DJ Asai
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA.
Organisms that have cilia or flagella express over a dozen dynein heavy chain genes. Of these heavy chain genes, most appear to encode axonemal dyneins, one encodes conventional cytoplasmic dynein (MAP1C or DHC1a), and one, here referred to as DHC1b, encodes an unclassified heavy chain. Previous analysis of sea urchin DHC1b (Gibbons et al. (1994) Mol. Biol. Cell 5, 57-70) indicated that this isoform is either an axonemal dynein with an unusual protein sequence or a cytoplasmic dynein whose expression increases during ciliogenesis. In the present study, we examined the expression of DHC1b in rat tissues. The DHC1b gene is expressed in all tissues examined, including unciliated liver and heart cells. In contrast, rat axonemal dyneins are only expressed in tissues that produce cilia or flagella. In cultured rat tracheal epithelial (RTE) cells, DHC1b is expressed in undifferentiated cells and increases in expression during ciliogenesis. In contrast, the expression of conventional cytoplasmic dynein, DHC1a, does not change during RTE differentiation and axonemal dynein is not expressed until after differentiation commences. In order to examine the expression of DHC1b protein, we produced an isoform-specific antibody to a synthetic peptide derived from the rat DHC1b sequence. The antibody demonstrated that DHC1b is a relatively minor component of partially purified cytoplasmic dynein. Indirect immunofluorescence microscopy revealed that DHC1b is not detected in cilia and remains in the cytoplasm of ciliated RTE cells, often accumulating at the apical ends of the cells. These results suggest that DHC1b is a cytoplasmic dynein that may participate in intracellular trafficking in polarized cells.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Huangfu and K. V. Anderson From the Cover: Cilia and Hedgehog responsiveness in the mouse PNAS, August 9, 2005; 102(32): 11325 - 11330. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Perrone, D. Tritschler, P. Taulman, R. Bower, B. K. Yoder, and M. E. Porter A Novel Dynein Light Intermediate Chain Colocalizes with the Retrograde Motor for Intraflagellar Transport at Sites of Axoneme Assembly in Chlamydomonas and Mammalian Cells Mol. Biol. Cell, May 1, 2003; 14(5): 2041 - 2056. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mikami, S. H. Tynan, T. Hama, K. Luby-Phelps, T. Saito, J. E. Crandall, J. C. Besharse, and R. B. Vallee Molecular structure of cytoplasmic dynein 2 and its distribution in neuronal and ciliated cells J. Cell Sci., March 14, 2003; 115(24): 4801 - 4808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Grissom, E. A. Vaisberg, and J. R. McIntosh Identification of a Novel Light Intermediate Chain (D2LIC) for Mammalian Cytoplasmic Dynein 2 Mol. Biol. Cell, March 1, 2002; 13(3): 817 - 829. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-Z. Chuang, T. A. Milner, and C.-H. Sung Subunit Heterogeneity of Cytoplasmic Dynein: Differential Expression of 14 kDa Dynein Light Chains in Rat Hippocampus J. Neurosci., August 1, 2001; 21(15): 5501 - 5512. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Reed, J. L. Carson, B. M. Moats-Staats, T. Lucier, P.-c. Hu, L. Brighton, T. M. Gambling, C.-H. Huang, M. W. Leigh, and A. M. Collier Characterization of an Axonemal Dynein Heavy Chain Expressed Early in Airway Epithelial Ciliogenesis Am. J. Respir. Cell Mol. Biol., December 1, 2000; 23(6): 734 - 741. [Abstract] [Full Text]
|
|
|
|
|
|
K. Boylan, M. Serr, and T. Hays A Molecular Genetic Analysis of the Interaction between the Cytoplasmic Dynein Intermediate Chain and the Glued (Dynactin) Complex Mol. Biol. Cell, November 1, 2000; 11(11): 3791 - 3803. [Abstract] [Full Text]
|
|
|
|
|
|
C. Valetti, D. M. Wetzel, M. Schrader, M. J. Hasbani, S. R. Gill, T. E. Kreis, and T. A. Schroer Role of Dynactin in Endocytic Traffic: Effects of Dynamitin Overexpression and Colocalization with CLIP-170 Mol. Biol. Cell, December 1, 1999; 10(12): 4107 - 4120. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. Signor, K. P. Wedaman, J. T. Orozco, N. D. Dwyer, C. I. Bargmann, L. S. Rose, and J. M. Scholey Role of a Class Dhc1b Dynein in Retrograde Transport of Ift Motors and Ift Raft Particles along Cilia, but Not Dendrites, in Chemosensory Neurons of Living Caenorhabditis elegans J. Cell Biol., November 1, 1999; 147(3): 519 - 530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Porter, R. Bower, J. A. Knott, P. Byrd, and W. Dentler Cytoplasmic Dynein Heavy Chain 1b Is Required for Flagellar Assembly in Chlamydomonas Mol. Biol. Cell, March 1, 1999; 10(3): 693 - 712. [Abstract] [Full Text]
|
|
|
|
|
|
S. Lee, J. C. Wisniewski, W. L. Dentler, and D. J. Asai Gene Knockouts Reveal Separate Functions for Two Cytoplasmic Dyneins in Tetrahymena thermophila Mol. Biol. Cell, March 1, 1999; 10(3): 771 - 784. [Abstract] [Full Text]
|
|
|
|
|
|
G. J. Pazour, B. L. Dickert, and G. B. Witman The DHC1b (DHC2) Isoform of Cytoplasmic Dynein Is Required for Flagellar Assembly J. Cell Biol., February 8, 1999; 144(3): 473 - 481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Rosenbaum, D. G. Cole, and D. R. Diener Intraflagellar Transport: The Eyes Have It J. Cell Biol., February 8, 1999; 144(3): 385 - 388. [Full Text] [PDF]
|
|
|
|
|
|
C Roghi and V. Allan Dynamic association of cytoplasmic dynein heavy chain 1a with the Golgi apparatus and intermediate compartment J. Cell Sci., January 12, 1999; 112(24): 4673 - 4685. [Abstract] [PDF]
|
|
|
|
|
|
G. J. Pazour, C. G. Wilkerson, and G. B. Witman A Dynein Light Chain Is Essential for the Retrograde Particle Movement of Intraflagellar Transport (IFT) J. Cell Biol., May 18, 1998; 141(4): 979 - 992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. S. Criswell and D. J. Asai Evidence for Four Cytoplasmic Dynein Heavy Chain Isoforms in Rat Testis Mol. Biol. Cell, February 1, 1998; 9(2): 237 - 247. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. L. Carson, W. Reed, T. Lucier, L. Brighton, T. M. Gambling, C.-H. Huang, and A. M. Collier Pre- and Postnatal Lung Development, Maturation, and Plasticity: Axonemal dynein expression in human fetal tracheal epithelium Am J Physiol Lung Cell Mol Physiol, March 1, 2002; 282(3): L421 - L430. [Abstract] [Full Text] [PDF]
|
|
