|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 109, Issue 8 2031-2040, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
JF Challacombe, DM Snow and PC Letourneau
University of Minnesota, Department of Cell Biology and Neuroanatomy, Minneapolis 55455, USA.
The extracellular matrix through which growth cones navigate contains molecules, such as chondroitin sulfate proteoglycan, that can inhibit growth cone advance and induce branching and turning. Growth cone turning is accompanied by rearrangement of the cytoskeleton. To identify changes in the organization of actin filaments and microtubules that occur as growth cones turn, we used time-lapse phase contrast videomicroscopy to observe embryonic chick dorsal root ganglion neuronal growth cones at a substratum border between fibronectin and chondroitin sulfate proteoglycan, in the presence and absence of cytochalasin B. Growth cones were fixed and immunocytochemically labeled to identify actin filaments and dynamic and stable microtubules. Our results suggest that microtubules are rearranged within growth cones to accomplish turning to avoid chondroitin sulfate proteoglycan. Compared to growth cones migrating on fibronectin, turning growth cones were more narrow, and they contained dynamic microtubules that were closer to the leading edge and were more bundled. Cytochalasin B-treated growth cones sidestepped laterally along the border instead of turning, and in sidestepping growth cones, microtubules were not bundled and aligned. We conclude that actin filament bundles are required for microtubule reorientation and growth cone turning to avoid chondroitin sulfate proteoglycan.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Geraldo and P. R. Gordon-Weeks Cytoskeletal dynamics in growth-cone steering J. Cell Sci., October 15, 2009; 122(20): 3595 - 3604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. H. Chadborn, A. I. Ahmed, M. R. Holt, R. Prinjha, G. A. Dunn, G. E. Jones, and B. J. Eickholt PTEN couples Sema3A signalling to growth cone collapse. J. Cell Sci., March 1, 2006; 119(Pt 5): 951 - 957. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Jablonka, K. Karle, B. Sandner, C. Andreassi, K. von Au, and M. Sendtner Distinct and overlapping alterations in motor and sensory neurons in a mouse model of spinal muscular atrophy Hum. Mol. Genet., February 1, 2006; 15(3): 511 - 518. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Bouquet, S. Soares, Y. von Boxberg, M. Ravaille-Veron, F. Propst, and F. Nothias Microtubule-Associated Protein 1B Controls Directionality of Growth Cone Migration and Axonal Branching in Regeneration of Adult Dorsal Root Ganglia Neurons J. Neurosci., August 11, 2004; 24(32): 7204 - 7213. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. J. Turner, S. Nicholls, and A. Hall The Activity of the Plexin-A1 Receptor Is Regulated by Rac J. Biol. Chem., August 6, 2004; 279(32): 33199 - 33205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. W. Grabham, B. Reznik, and D. J. Goldberg Microtubule and Rac 1-dependent F-actin in growth cones J. Cell Sci., September 15, 2003; 116(18): 3739 - 3748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. W. Schaefer, N. Kabir, and P. Forscher Filopodia and actin arcs guide the assembly and transport of two populations of microtubules with unique dynamic parameters in neuronal growth cones J. Cell Biol., July 8, 2002; 158(1): 139 - 152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.-Q. Zhou, C. M. Waterman-Storer, and C. S. Cohan Focal loss of actin bundles causes microtubule redistribution and growth cone turning J. Cell Biol., May 28, 2002; 157(5): 839 - 849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Noiges, R. Eichinger, W. Kutschera, I. Fischer, Z. Nemeth, G. Wiche, and F. Propst Microtubule-Associated Protein 1A (MAP1A) and MAP1B: Light Chains Determine Distinct Functional Properties J. Neurosci., March 15, 2002; 22(6): 2106 - 2114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. W. Dent and K. Kalil Axon Branching Requires Interactions between Dynamic Microtubules and Actin Filaments J. Neurosci., December 15, 2001; 21(24): 9757 - 9769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.-q. Zhou and C. S. Cohan Growth Cone Collapse through Coincident Loss of Actin Bundles and Leading Edge Actin without Actin Depolymerization J. Cell Biol., May 28, 2001; 153(5): 1071 - 1084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Lautermilch and N. C. Spitzer Regulation of Calcineurin by Growth Cone Calcium Waves Controls Neurite Extension J. Neurosci., January 1, 2000; 20(1): 315 - 325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Steketee and K. W. Tosney Contact with Isolated Sclerotome Cells Steers Sensory Growth Cones by Altering Distinct Elements of Extension J. Neurosci., May 1, 1999; 19(9): 3495 - 3506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Prokop, J. Uhler, J. Roote, and M. Bate The kakapo Mutation Affects Terminal Arborization and Central Dendritic Sprouting of Drosophila Motorneurons J. Cell Biol., November 30, 1998; 143(5): 1283 - 1294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Togel, G. Wiche, and F. Propst Novel Features of the Light Chain of Microtubule-associated Protein MAP1B: Microtubule Stabilization, Self Interaction, Actin Filament Binding, and Regulation by the Heavy Chain J. Cell Biol., November 2, 1998; 143(3): 695 - 707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Suter, L. D. Errante, V. Belotserkovsky, and P. Forscher The Ig Superfamily Cell Adhesion Molecule, apCAM, Mediates Growth Cone Steering by Substrate-Cytoskeletal Coupling J. Cell Biol., April 6, 1998; 141(1): 227 - 240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Wilsch-Brauninger, H. Schwarz, and C. Nusslein-Volhard A Sponge-like Structure Involved in the Association and Transport of Maternal Products during Drosophila Oogenesis J. Cell Biol., November 3, 1997; 139(3): 817 - 829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Challacombe, D. M. Snow, and P. C. Letourneau Dynamic Microtubule Ends Are Required for Growth Cone Turning to Avoid an Inhibitory Guidance Cue J. Neurosci., May 1, 1997; 17(9): 3085 - 3095. [Abstract] [Full Text] [PDF]
|
|
