|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 109, Issue 8 2079-2088, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
P Kaldis, M Stolz, M Wyss, E Zanolla, B Rothen-Rutishauser, T Vorherr and T Wallimann
Swiss Federal Institute of Technology, Institute for Cell Biology, ETH-Honggerberg, Zurich, Switzerland.
The creatine kinase (CK) isoenzyme system is essential for motility in rooster and sea urchin sperm. In the present study, biochemical characterization as well as immunofluorescence and confocal laser microscopy with highly specific antibodies against various chicken CK isoenzymes revealed that cytosolic brain-type CK isoenzyme (B-CK) is the only CK isoenzyme in rooster seminal plasma, while three isoenzymes, cytosolic B-CK, sarcomeric mitochondrial CK (Mib-CK), and a variant of ubiquitous Mi-CK ('Mia-CK variant'), are found in rooster spermatozoa. These three isoenzymes are localized in different regions of the sperm cell. B-CK and Mib-CK were localized along the entire sperm tail and in the mitochondria-rich midpiece, respectively. The 'Mia-CK variant', on the other hand, was found predominantly at the head-midpiece boundary, in a non-uniform manner in the midpiece itself and, surprisingly, at the distal end of the sperm tail as well as at the acrosome. Several lines of evidence show that the 'Mia-CK variant' shares some characteristics with purified Mia-CK from chicken brain, but also displays distinctive features. This is the first evidence for two different Mi-CK isoenzymes occurring in one cell and, additionally, for the co-expression of Mib-CK and cytosolic brain-type B-CK in the same cell. The relevance of these findings for sperm physiology and energetics is discussed.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Feiden, H. Stypa, U. Wolfrum, G. Wegener, and G. Kamp A novel pyruvate kinase (PK-S) from boar spermatozoa is localized at the fibrous sheath and the acrosome Reproduction, July 1, 2007; 134(1): 81 - 95. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. E. Meyer, L. B. Machado, A. P. S. A. Santiago, W. S. da-Silva, F. G. De Felice, O. Holub, M. F. Oliveira, and A. Galina Mitochondrial Creatine Kinase Activity Prevents Reactive Oxygen Species Generation: ANTIOXIDANT ROLE OF MITOCHONDRIAL KINASE-DEPENDENT ADP RE-CYCLING ACTIVITY J. Biol. Chem., December 8, 2006; 281(49): 37361 - 37371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Ginger, E. S. Ngazoa, C. A. Pereira, T. J. Pullen, M. Kabiri, K. Becker, K. Gull, and D. Steverding Intracellular Positioning of Isoforms Explains an Unusually Large Adenylate Kinase Gene Family in the Parasite Trypanosoma brucei J. Biol. Chem., March 25, 2005; 280(12): 11781 - 11789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Noguchi, T Sawada, and T Akazawa ATP-regenerating system in the cilia of Paramecium caudatum J. Exp. Biol., January 3, 2001; 204(6): 1063 - 1071. [Abstract] [PDF]
|
|
|
|
 |
|
 K. E. Ross, P. Kaldis, and M. J. Solomon Activating Phosphorylation of the Saccharomyces cerevisiae Cyclin-dependent Kinase, Cdc28p, Precedes Cyclin Binding Mol. Biol. Cell, May 1, 2000; 11(5): 1597 - 1609. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y.-m. E. Wang, P. Esbensen, and D. Bentley Arginine Kinase Expression and Localization in Growth Cone Migration J. Neurosci., February 1, 1998; 18(3): 987 - 998. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D Westhoff and G Kamp Glyceraldehyde 3-phosphate dehydrogenase is bound to the fibrous sheath of mammalian spermatozoa J. Cell Sci., January 8, 1997; 110(15): 1821 - 1829. [Abstract] [PDF]
|
|
