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Journal of Cell Science, Vol 109, Issue 8 2161-2168, Copyright © 1996 by Company of Biologists
JOURNAL ARTICLES |
A Giese, MA Loo, SA Norman, S Treasurywala and ME Berens
Department of Neurosurgery, University Hospital Eppendorf, Hamburg, Germany.
Tenascin, an extracellular matrix protein, is expressed in human gliomas in vitro and in vivo. The distribution of tenascin at the invasive edge of these tumors, even surrounding solitary invading cells, suggests a role for this protein as a regulator of glioma cell migration. We tested whether purified tenascin, passively deposited on surfaces, influenced the adhesion or migration of a human gliomaderived cell line, SF-767. Adhesion of glioma cells to tenascin increased in a dose-dependent fashion up to a coating concentration of 10 micrograms/ml. Higher coating concentrations resulted in progressively fewer cells attaching. Cell adhesion could be blocked to basal levels using anti-beta 1 integrin antibodies. In contrast, when anti-alpha v antibodies were added to the medium of cells on tenascin, cell adhesion was enhanced slightly. Using a microliter scale migration assay, we found that cell motility on tenascin was dose dependently stimulated at coating concentrations of 1 and 3 micrograms/ml, but migration was inhibited below levels of non-specific motility when tested at coating concentrations of 30 and 100 micrograms/ml. Migration on permissive concentrations of tenascin could be reversibly inhibited with anti-beta 1, while treatment with anti-alpha v antibodies increased migration rates. We conclude that SF-767 glioma cells express two separate integrin receptors that mediate contrasting adhesive and migratory responses to tenascin.
This article has been cited by other articles:
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  S. Sarkar, R. K. Nuttall, S. Liu, D. R. Edwards, and V. W. Yong Tenascin-C Stimulates Glioma Cell Invasion through Matrix Metalloproteinase-12 Cancer Res., December 15, 2006; 66(24): 11771 - 11780. [Abstract] [Full Text] [PDF]
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B. Salhia, F. Rutten, M. Nakada, C. Beaudry, M. Berens, A. Kwan, and J. T. Rutka Inhibition of Rho-Kinase Affects Astrocytoma Morphology, Motility, and Invasion through Activation of Rac1 Cancer Res., October 1, 2005; 65(19): 8792 - 8800. [Abstract] [Full Text] [PDF]
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 A. M. Joy, C. E. Beaudry, N. L. Tran, F. A. Ponce, D. R. Holz, T. Demuth, and M. E. Berens Migrating glioma cells activate the PI3-K pathway and display decreased susceptibility to apoptosis J. Cell Sci., November 1, 2003; 116(21): 4409 - 4417. [Abstract] [Full Text] [PDF]
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 A. Giese, R. Bjerkvig, M.E. Berens, and M. Westphal Cost of Migration: Invasion of Malignant Gliomas and Implications for Treatment J. Clin. Oncol., April 15, 2003; 21(8): 1624 - 1636. [Abstract] [Full Text] [PDF]
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 A. Giese, C. Hagel, E. L. Kim, S. Zapf, J. Djawaheri, M. E. Berens, and M. Westphal Thromboxane synthase regulates the migratory phenotype of human glioma cells Neuro-oncol, January 1, 1999; 1(1): 3 - 13. [Abstract] [PDF]
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G. Phillips, L. Krushel, and K. Crossin Domains of tenascin involved in glioma migration J. Cell Sci., January 4, 1998; 111(8): 1095 - 1104. [Abstract] [PDF]
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