|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 11, 557-568, Copyright © 1972 by Company of Biologists
Submitted on February 3, 1972
1 Max-Planck-Institut für Virusforschung, Molekularbiologische Abteilung, 74 Tübingen, W. Germany
2 Department of Developmental and Cell Biology, University of California, Irvine, California 92664, U.S.A.
The cell cycle parameters of epithelial cells of Hydra attenuata are described. Specifically the rate of proliferation and the fraction of proliferating cells have been determined under conditions of defined growth rate. Techniques involved standard methods of cell cycle analysis using histological and tissue maceration preparations; pulse-chase and continuous labelling with [3H]thymidine followed by autoradiographic analysis, and microspectrophotometric determination of nuclear DNA content in single cells. The results indicate that more than 90% of hydra epithelial cells are actively proliferating with a cell cycle duration about equal to the tissue doubling time. In well fed hydra the average cell cycle is about 3 days long. S period is 12-15 h, G1 0-1 h, and mitosis 1.5 h. Most of the cell cycle consists of a long G2 period of variable duration (24-72 h). The results provide no evidence for a subpopulation of rapidly proliferating cells as predicted by ‘growth zone’ models of hydra morphogenesis. The results also indicate that the population of epithelial cells is self-sustaining requiring no input by differentiation from other cell types. The long and variable G2 period means that DNA synthesis and the following cell division are effectively uncoupled such that inhibitors of DNA synthesis may not stop epithelial cell division. The variable nature of the G2 period suggests it as a possible point of control of hydra growth.
Submitted on February 3, 1972
This article has been cited by other articles:
|
|
 |
|
  J. Wittlieb, K. Khalturin, J. U. Lohmann, F. Anton-Erxleben, and T. C. G. Bosch From the Cover: Transgenic Hydra allow in vivo tracking of individual stem cells during morphogenesis PNAS, April 18, 2006; 103(16): 6208 - 6211. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Broun, L. Gee, B. Reinhardt, and H. R. Bode Formation of the head organizer in hydra involves the canonical Wnt pathway Development, June 15, 2005; 132(12): 2907 - 2916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Takahashi, M. Hatta, S. Yum, L. Gee, M. Ohtani, T. Fujisawa, and H. R. Bode Hym-301, a novel peptide, regulates the number of tentacles formed in hydra Development, May 1, 2005; 132(9): 2225 - 2234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Broun and H. R. Bode Characterization of the head organizer in hydra Development, March 4, 2003; 129(4): 875 - 884. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. R. Bode The Role of Hox Genes in Axial Patterning in Hydra Integr. Comp. Biol., June 1, 2001; 41(3): 621 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N Harafuji, T Takahashi, M Hatta, H Tezuka, F Morishita, O Matsushima, and T Fujisawa Enhancement of foot formation in Hydra by a novel epitheliopeptide, Hym-323 Development, January 2, 2001; 128(3): 437 - 446. [Abstract] [PDF]
|
|
|
|
 |
|
 J. U. Lohmann and T. C.G. Bosch The novel peptide HEADY specifies apical fate in a simple radially symmetric metazoan Genes & Dev., November 1, 2000; 14(21): 2771 - 2777. [Abstract] [Full Text]
|
|
|
|
|
|
K. Smith, L Gee, and H. Bode HyAlx, an aristaless-related gene, is involved in tentacle formation in hydra Development, January 11, 2000; 127(22): 4743 - 4752. [Abstract] [PDF]
|
|
|
|
|
|
T Takahashi, O Koizumi, Y Ariura, A Romanovitch, T. Bosch, Y Kobayakawa, S Mohri, H. Bode, S Yum, M Hatta, et al. A novel neuropeptide, Hym-355, positively regulates neuron differentiation in Hydra Development, January 3, 2000; 127(5): 997 - 1005. [Abstract] [PDF]
|
|
|
|
|
|
A Grens, H Shimizu, S. Hoffmeister, H. Bode, and T Fujisawa The novel signal peptides, pedibin and Hym-346, lower positional value thereby enhancing foot formation in hydra Development, January 2, 1999; 126(3): 517 - 524. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Bode The interstitial cell lineage of hydra: a stem cell system that arose early in evolution J. Cell Sci., January 6, 1996; 109(6): 1155 - 1164. [PDF]
|
|
|
|
|
|
I Scheurlen, S. Hoffmeister, and H. Schaller Presence and expression of G2 cyclins in the coelenterate hydra J. Cell Sci., January 5, 1996; 109(5): 1063 - 1069. [Abstract] [PDF]
|
|
|
|
|
|
A Grens, E Mason, J. Marsh, and H. Bode Evolutionary conservation of a cell fate specification gene: the Hydra achaete-scute homolog has proneural activity in Drosophila Development, January 12, 1995; 121(12): 4027 - 4035. [Abstract] [PDF]
|
|
