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Journal of Cell Science, Vol 11, 669-673, Copyright © 1972 by Company of Biologists

Submitted on March 27, 1972

Sodium-Induced Changes in the Nuclei of Monolayer Hela Cultures

K. JOBST 1 and N. KELLERMAYER 1

1 Department of Clinical Chemistry, Medical University of Pécs, Pécs, Hungary

In HeLa cells cultured in a medium containing 142 mequiv./l. sodium ions only the mitotic forms are birefringent (anisotropic index, 27/1000). In a culture medium containing 155-160 mequiv./l. sodium ions, large numbers of nuclear forms reminiscent of the prophase were found. In such a medium the anisotropic index was 86/1000. At low sodium ion concentration (90-110 mequiv./l.) these anomalous, prophase-like forms were not seen and the anisotropic index was 18/1000. The appearance of the birefringent, prophase-like forms is related to a structural rearrangement and condensation of DNP in a hypertonic medium.

Submitted on March 27, 1972






© The Company of Biologists Ltd 1972