|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 110, Issue 1 95-103, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
M Marsh and R Bron
MRC Laboratory for Molecular Cell Biology, University College London, UK. m.marsh@ucl.ac.uk
Alphaviruses, such as Semliki Forest virus, normally enter cells by penetration from acidic organelles of the endocytic pathway. The virions are internalised intact from the cell surface before undergoing acid-induced fusion in endosomes. To investigate the possibility that endocytosis might play a role in delivering virions to specific sites for replication, we compared SFV infection of baby hamster kidney (BHK) cells and Chinese hamster ovary (CHO) cells following either normal virus fusion in endosomes or experimentally-induced fusion at the cell surface. Whereas baby hamster kidney cells were infected efficiently following fusion in endosomes or at the plasma membrane, Chinese hamster ovary cells were only infected following fusion from endocytic organelles. Virions fused at the plasma membrane of CHO cells failed to initiate viral RNA and protein synthesis. Similar results were observed when CHO cells were challenged with a rhabdovirus, vesicular stomatitis virus. These data suggest that in certain cell types a barrier, other than the plasma membrane, can prevent infection by alpha- and rhabdoviruses fused at the cell surface. Moreover, they suggest the endocytic pathway provides a mechanism for bringing viral particles to a site, or sites, in the cell where replication can proceed.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Zhu, D. Yu, and X. Zhang The Spike Protein of Murine Coronavirus Regulates Viral Genome Transport from the Cell Surface to the Endoplasmic Reticulum during Infection J. Virol., October 15, 2009; 83(20): 10653 - 10663. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Bertrand, M. Cote, Y.-M. Zheng, L. M. Albritton, and S.-L. Liu Jaagsiekte Sheep Retrovirus Utilizes a pH-Dependent Endocytosis Pathway for Entry J. Virol., March 1, 2008; 82(5): 2555 - 2559. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. M. van der Schaar, M. J. Rust, B.-L. Waarts, H. van der Ende-Metselaar, R. J. Kuhn, J. Wilschut, X. Zhuang, and J. M. Smit Characterization of the Early Events in Dengue Virus Cell Entry by Biochemical Assays and Single-Virus Tracking J. Virol., November 1, 2007; 81(21): 12019 - 12028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. C. Chu, L. J. McElroy, V. Chu, B. E. Bauman, and G. R. Whittaker The Avian Coronavirus Infectious Bronchitis Virus Undergoes Direct Low-pH-Dependent Fusion Activation during Entry into Host Cells J. Virol., April 1, 2006; 80(7): 3180 - 3188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Tscherne, C. T. Jones, M. J. Evans, B. D. Lindenbach, J. A. McKeating, and C. M. Rice Time- and Temperature-Dependent Activation of Hepatitis C Virus for Low-pH-Triggered Entry J. Virol., February 15, 2006; 80(4): 1734 - 1741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. B. Milne, A. V. Nicola, J. C. Whitbeck, R. J. Eisenberg, and G. H. Cohen Glycoprotein D Receptor-Dependent, Low-pH-Independent Endocytic Entry of Herpes Simplex Virus Type 1 J. Virol., June 1, 2005; 79(11): 6655 - 6663. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Campbell, R. Nunez, and T. J. Hope Disruption of the Actin Cytoskeleton Can Complement the Ability of Nef To Enhance Human Immunodeficiency Virus Type 1 Infectivity J. Virol., June 1, 2004; 78(11): 5745 - 5755. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. Smith and A. Helenius How Viruses Enter Animal Cells Science, April 9, 2004; 304(5668): 237 - 242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Sun and G. R. Whittaker Role for Influenza Virus Envelope Cholesterol in Virus Entry and Infection J. Virol., December 1, 2003; 77(23): 12543 - 12551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Inoue, J. Winter, R. B. Lal, M. K. Offermann, and S. Koyano Characterization of Entry Mechanisms of Human Herpesvirus 8 by Using an Rta-Dependent Reporter Cell Line J. Virol., July 15, 2003; 77(14): 8147 - 8152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Sieczkarski, H. A. Brown, and G. R. Whittaker Role of Protein Kinase C {beta}II in Influenza Virus Entry via Late Endosomes J. Virol., January 1, 2003; 77(1): 460 - 469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Diaz-Griffero, S. A. Hoschander, and J. Brojatsch Endocytosis Is a Critical Step in Entry of Subgroup B Avian Leukosis Viruses J. Virol., December 15, 2002; 76(24): 12866 - 12876. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. B. Sieczkarski and G. R. Whittaker Dissecting virus entry via endocytosis J. Gen. Virol., June 1, 2002; 83(7): 1535 - 1545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Chazal, G. Singer, C. Aiken, M.-L. Hammarskjold, and D. Rekosh Human Immunodeficiency Virus Type 1 Particles Pseudotyped with Envelope Proteins That Fuse at Low pH No Longer Require Nef for Optimal Infectivity J. Virol., April 15, 2001; 75(8): 4014 - 4018. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Y. Nakano, K. Boucke, M. Suomalainen, R. P. Stidwill, and U. F. Greber The First Step of Adenovirus Type 2 Disassembly Occurs at the Cell Surface, Independently of Endocytosis and Escape to the Cytosol J. Virol., August 1, 2000; 74(15): 7085 - 7095. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Valetti, D. M. Wetzel, M. Schrader, M. J. Hasbani, S. R. Gill, T. E. Kreis, and T. A. Schroer Role of Dynactin in Endocytic Traffic: Effects of Dynamitin Overexpression and Colocalization with CLIP-170 Mol. Biol. Cell, December 1, 1999; 10(12): 4107 - 4120. [Abstract] [Full Text]
|
|
|
|
|
|
N. Bayer, D. Schober, E. Prchla, R. F. Murphy, D. Blaas, and R. Fuchs Effect of Bafilomycin A1 and Nocodazole on Endocytic Transport in HeLa Cells: Implications for Viral Uncoating and Infection J. Virol., December 1, 1998; 72(12): 9645 - 9655. [Abstract] [Full Text] [PDF]
|
|
