spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vignoud, L.
Right arrow Articles by Block, M. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vignoud, L.
Right arrow Articles by Block, M. R.

Journal of Cell Science, Vol 110, Issue 12 1421-1430, Copyright © 1997 by Company of Biologists

JOURNAL ARTICLES

NPXY motifs control the recruitment of the alpha5beta1 integrin in focal adhesions independently of the association of talin with the beta1 chain

L Vignoud, C Albiges-Rizo, P Frachet and MR Block
LEDAC/UMR CNRS-UJF 5538, Institut Albert Bonniot, Faculte de Medecine, La Tronche, France.

With the exception of the divergent beta4 and beta8 chains, the integrin beta subunit cytoplasmic domains are short and highly conserved sequences. Consensus motifs are found among the different cytoplasmic beta chains. Experiments using chimeric receptors demonstrated that the 47 amino acids of the beta1 subunit cytoplasmic domain contain sufficient information to target integrins to adhesion plaques. Three clusters of amino acids, named cyto-1, cyto-2 and cyto-3, seem to contribute to this localization. Cyto-2 and cyto-3 exhibit NPXY motifs. At present, the exact function of these motifs remains unknown but it is likely that these sequences are involved in protein-protein interactions. Although NPXY motifs often act as internalization signals at the cytoplasmic tail of membrane receptors, our previous results showed that the two NPXY motifs are not responsible for the alpha5beta1 integrin endocytosis. Herein, we address the question of the role of the two highly conserved NPXY motifs found in the beta1 cytoplasmic domain, and which correspond to the conserved domains cyto-2 and cyto-3. We demonstrate that, within the integrin beta1 cytoplasmic tail, the two NPXY motifs are required for the recruitment of the integrin in focal adhesions. In addition, our results indicate that these two motifs control but do not belong to the talin-binding sites. Finally, the analysis of the phenotypes of NPXY mutants reveals that the interaction of talin with the beta1 cytosolic domain is not sufficient to target the integrins to focal adhesions.


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
G. Tanentzapf, M. D. Martin-Bermudo, M. S. Hicks, and N. H. Brown
Multiple factors contribute to integrin-talin interactions in vivo
J. Cell Sci., April 15, 2006; 119(8): 1632 - 1644.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
A. Wiedemann, J. C. Patel, J. Lim, A. Tsun, Y. van Kooyk, and E. Caron
Two distinct cytoplasmic regions of the {beta}2 integrin chain regulate RhoA function during phagocytosis.
J. Cell Biol., March 27, 2006; 172(7): 1069 - 1079.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. A. Rosenthal-Allieri, M. Ticchioni, J. P. Breittmayer, Y. Shimizu, and A. Bernard
Influence of {beta}1 Integrin Intracytoplasmic Domains in the Regulation of VLA-4-Mediated Adhesion of Human T Cells to VCAM-1 under Flow Conditions
J. Immunol., July 15, 2005; 175(2): 1214 - 1223.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Bouvard, L. Vignoud, S. Dupe-Manet, N. Abed, H.-N. Fournier, C. Vincent-Monegat, S. F. Retta, R. Fassler, and M. R. Block
Disruption of Focal Adhesions by Integrin Cytoplasmic Domain-associated Protein-1alpha
J. Biol. Chem., February 14, 2003; 278(8): 6567 - 6574.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. Levy, S. Broad, D. Diekmann, R. D. Evans, and F. M. Watt
beta 1 Integrins Regulate Keratinocyte Adhesion and Differentiation by Distinct Mechanisms
Mol. Biol. Cell, February 1, 2000; 11(2): 453 - 466.
[Abstract] [Full Text]

Home page
 J. Virol.Home page
M. J. Hewish, Y. Takada, and B. S. Coulson
Integrins alpha 2beta 1 and alpha 4beta 1 Can Mediate SA11 Rotavirus Attachment and Entry into Cells
J. Virol., January 1, 2000; 74(1): 228 - 236.
[Abstract] [Full Text]

Home page
 Genes Dev.Home page
D. S. Rice and T. Curran
Mutant mice with scrambled brains: understanding the signaling pathways that control cell positioning in the CNS
Genes & Dev., November 1, 1999; 13(21): 2758 - 2773.
[Full Text]

Home page
 J. Biol. Chem.Home page
S. Patil, A. Jedsadayanmata, J. D. Wencel-Drake, W. Wang, I. Knezevic, and S. C.-T. Lam
Identification of a Talin-binding Site in the Integrin beta 3 Subunit Distinct from the NPLY Regulatory Motif of Post-ligand Binding Functions. THE TALIN N-TERMINAL HEAD DOMAIN INTERACTS WITH THE MEMBRANE-PROXIMAL REGION OF THE beta 3 CYTOPLASMIC TAIL
J. Biol. Chem., October 1, 1999; 274(40): 28575 - 28583.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Gut, M. S. Balda, and K. Matter
The Cytoplasmic Domains of a beta 1 Integrin Mediate Polarization in Madin-Darby Canine Kidney Cells by Selective Basolateral Stabilization
J. Biol. Chem., November 6, 1998; 273(45): 29381 - 29388.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. C. Romzek, E. S. Harris, C. L. Dell, J. Skronek, E. Hasse, P. J. Reynolds, S. W. Hunt III, and Y. Shimizu
Use of a beta 1 Integrin-deficient Human T Cell to Identify beta 1 Integrin Cytoplasmic Domain Sequences Critical for Integrin Function
Mol. Biol. Cell, October 1, 1998; 9(10): 2715 - 2727.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
J.-M. Heraud, C. Racaud-Sultan, D. Gironcel, C. Albiges-Rizo, T. Giacomini, S. Roques, V. Martel, M. Breton-Douillon, B. Perret, and H. Chap
Lipid Products of Phosphoinositide 3-Kinase and Phosphatidylinositol 4',5'-Bisphosphate Are Both Required for ADP-dependent Platelet Spreading
J. Biol. Chem., July 10, 1998; 273(28): 17817 - 17823.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Schaffner-Reckinger, V. Gouon, C. Melchior, S. Plancon, and N. Kieffer
Distinct Involvement of beta 3 Integrin Cytoplasmic Domain Tyrosine Residues 747 and 759 in Integrin-mediated Cytoskeletal Assembly and Phosphotyrosine Signaling
J. Biol. Chem., May 15, 1998; 273(20): 12623 - 12632.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
T. Sakai, Q. Zhang, R. Fassler, and D. F. Mosher
Modulation of beta 1A Integrin Functions by Tyrosine Residues in the beta 1 Cytoplasmic Domain
J. Cell Biol., April 20, 1998; 141(2): 527 - 538.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Martel, C. Racaud-Sultan, S. Dupe, C. Marie, F. Paulhe, A. Galmiche, M. R. Block, and C. Albiges-Rizo
Conformation, Localization, and Integrin Binding of Talin Depend on Its Interaction with Phosphoinositides
J. Biol. Chem., June 8, 2001; 276(24): 21217 - 21227.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. L. Jannuzi, T. A. Bunch, M. C. Brabant, S. W. Miller, L. Mukai, M. Zavortink, and D. L. Brower
Disruption of C-Terminal Cytoplasmic Domain of beta PS Integrin Subunit Has Dominant Negative Properties in Developing Drosophila
Mol. Biol. Cell, April 1, 2002; 13(4): 1352 - 1365.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1997