spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Avery, J.
Right arrow Articles by Whitaker, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Avery, J.
Right arrow Articles by Whitaker, M.

Journal of Cell Science, Vol 110, Issue 14 1555-1561, Copyright © 1997 by Company of Biologists

JOURNAL ARTICLES

In vitro exocytosis in sea urchin eggs requires a synaptobrevin-related protein

J Avery, A Hodel and M Whitaker
Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne, UK.

Sea urchin eggs provide an efficient in vitro model of exocytosis. We have identified proteins in sea urchin eggs that cross-react with antibodies to mammalian synaptobrevin, synaptotagmin, SNAP-25, syntaxin and rab3a. We show that these proteins are localized to the sea urchin egg cortex, using western blotting and immunocytochemistry. Tetanus toxin light chain cleaves the synaptobrevin-related protein in vitro and inhibits calcium-induced exocytosis. These data demonstrate a conservation between phyla of protein sequence and molecular mechanisms thought to facilitate exocytosis and show that the sea urchin egg provides a unique in vitro exocytotic model with which to study the conserved protein machinery of membrane fusion during secretion.


This article has been cited by other articles:


Home page
 Ann. N. Y. Acad. Sci.Home page
R. A. STEINHARDT
The Mechanisms of Cell Membrane Repair: A Tutorial Guide to Key Experiments
Ann. N.Y. Acad. Sci., December 1, 2005; 1066(1): 152 - 165.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Whalley, K. Timmers, J. Coorssen, L. Bezrukov, D. H. Kingsley, and J. Zimmerberg
Membrane fusion of secretory vesicles of the sea urchin egg in the absence of NSF
J. Cell Sci., May 1, 2004; 117(11): 2345 - 2356.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
T. Togo, J. M. Alderton, and R. A. Steinhardt
Long-Term Potentiation of Exocytosis and Cell Membrane Repair in Fibroblasts
Mol. Biol. Cell, January 1, 2003; 14(1): 93 - 106.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
J. Grosse, A. Bulling, C. Brucker, U. Berg, A. Amsterdam, A. Mayerhofer, and M. Gratzl
Synaptosome-Associated Protein of 25 Kilodaltons in Oocytes and Steroid-Producing Cells of Rat and Human Ovary: Molecular Analysis and Regulation by Gonadotropins
Biol Reprod, August 1, 2000; 63(2): 643 - 650.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
M. Tahara, J. R. Coorssen, K. Timmers, P. S. Blank, T. Whalley, R. Scheller, and J. Zimmerberg
Calcium Can Disrupt the SNARE Protein Complex on Sea Urchin Egg Secretory Vesicles without Irreversibly Blocking Fusion
J. Biol. Chem., December 11, 1998; 273(50): 33667 - 33673.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1997