|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 110, Issue 15 1693-1704, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
AB Verkhovsky, TM Svitkina and GG Borisy
Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA. P2
The polarity of actin filaments is fundamental for the subcellular mechanics of actin-myosin interaction; however, little is known about how actin filaments are oriented with respect to myosin in non-muscle cells and how actin polarity organization is established and maintained. Here we approach these questions by investigating changes in the organization and polarity of actin relative to myosin II during actin filament translocation. Actin and myosin II reorganization was followed both kinetically, using microinjected fluorescent analogs of actin and myosin, and ultrastructurally, using myosin S1 decoration and immunogold labelling, in cultured fibroblasts that were induced to contract by treatment with cytochalasin D. We observed rapid (within 15 minutes) formation of ordered actin filament arrays: short tapered bundles and aster-like assemblies, in which filaments had uniform polarity with their barbed ends oriented toward the aggregate of myosin II at the base of a bundle or in the center of an aster. The resulting asters further interacted with each other and aggregated into bigger asters. The arrangement of actin in asters was in sharp contrast to the mixed polarity of actin filaments relative to myosin in non-treated cells. At the edge of the cell, actin filaments became oriented with their barbed ends toward the cell center; that is, the orientation was opposite to what was observed at the edge of nontreated cells. This rearrangement is indicative of relative translocation of actin and myosin II and of the ability of myosin II to sort actin filaments with respect to their polarity during translocation. The results suggest that the myosin II-actin system of non-muscle cells is organized as a dynamic network where actin filament arrangement is defined in the course of its interaction with myosin II.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Nagaya, T. Tamura, A. Higa-Nishiyama, K. Ohashi, M. Takeuchi, H. Hashimoto, K. Hatsuzawa, M. Kinjo, T. Okada, and I. Wada Regulated motion of glycoproteins revealed by direct visualization of a single cargo in the endoplasmic reticulum J. Cell Biol., January 10, 2008; 180(1): 129 - 143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yamada and W. J. Nelson Localized zones of Rho and Rac activities drive initiation and expansion of epithelial cell cell adhesion J. Cell Biol., July 24, 2007; 178(3): 517 - 527. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Haviv, Y. Brill-Karniely, R. Mahaffy, F. Backouche, A. Ben-Shaul, T. D. Pollard, and A. Bernheim-Groswasser Reconstitution of the transition from lamellipodium to filopodium in a membrane-free system PNAS, March 28, 2006; 103(13): 4906 - 4911. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. I. Ivanov, D. Hunt, M. Utech, A. Nusrat, and C. A. Parkos Differential Roles for Actin Polymerization and a Myosin II Motor in Assembly of the Epithelial Apical Junctional Complex Mol. Biol. Cell, June 1, 2005; 16(6): 2636 - 2650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. J. Standeven, L. M. Carlin, P. Borszcz, D. M. Davis, and D. N. Burshtyn The Actin Cytoskeleton Controls the Efficiency of Killer Ig-Like Receptor Accumulation at Inhibitory NK Cell Immune Synapses J. Immunol., November 1, 2004; 173(9): 5617 - 5625. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. ENDLICH, K. R. KRESS, J. REISER, D. UTTENWEILER, W. KRIZ, P. MUNDEL, and K. ENDLICH Podocytes Respond to Mechanical Stress In Vitro J. Am. Soc. Nephrol., March 1, 2001; 12(3): 413 - 422. [Abstract] [Full Text]
|
|
|
|
 |
|
 T Wakatsuki, B Schwab, N. Thompson, and E. Elson Effects of cytochalasin D and latrunculin B on mechanical properties of cells J. Cell Sci., January 3, 2001; 114(5): 1025 - 1036. [Abstract] [PDF]
|
|
|
|
|
|
Q. Wei and R. S. Adelstein Conditional Expression of a Truncated Fragment of Nonmuscle Myosin II-A Alters Cell Shape but Not Cytokinesis in HeLa Cells Mol. Biol. Cell, October 1, 2000; 11(10): 3617 - 3627. [Abstract] [Full Text]
|
|
|
|
|
|
M. W. Rochlin, M. E. Dailey, and P. C. Bridgman Polymerizing Microtubules Activate Site-directed F-Actin Assembly in Nerve Growth Cones Mol. Biol. Cell, July 1, 1999; 10(7): 2309 - 2327. [Abstract] [Full Text]
|
|
|
|
|
|
R. Christopher, S. Judge, P. Vincent, P. Higgins, and P. McKeown-Longo The amino-terminal matrix assembly domain of fibronectin stabilizes cell shape and prevents cell cycle progression J. Cell Sci., January 10, 1999; 112(19): 3225 - 3235. [Abstract] [PDF]
|
|
|
|
|
|
C. L. Adams, Y.-T. Chen, S. J Smith, and W. James Nelson Mechanisms of Epithelial Cell-Cell Adhesion and Cell Compaction Revealed by High-resolution Tracking of E-Cadherin- Green Fluorescent Protein J. Cell Biol., August 24, 1998; 142(4): 1105 - 1119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Svitkina, A. B. Verkhovsky, K. M. McQuade, and G. G. Borisy Analysis of the Actin-Myosin II System in Fish Epidermal Keratocytes: Mechanism of Cell Body Translocation J. Cell Biol., October 20, 1997; 139(2): 397 - 415. [Abstract] [Full Text] [PDF]
|
|
