|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 110, Issue 15 1767-1780, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
H Palokangas, M Mulari and HK Vaananen
Department of Anatomy and Biocenter, University of Oulu, Finland. htp@raita.oulu.fi
We have characterized the convoluted ruffled border (RB) membrane that an activated osteoclast maintains against the bone matrix. The bulk of both lgp110 and rab7, a small GTP-binding protein participating in vesicle fusion to late endosomes, was localized to the RB. This indicates that the membrane has some characteristics of late endosomal membranes in other cells. Furthermore, the bulk of membrane-bound rab7 on the RB suggests that endocytic membrane transport is oriented towards the RB in resorbing osteoclasts. Consistently, both lumenal horseradish peroxidase and receptor-bound transferrin, a marker of the early endosomal recycling pathway, were efficiently endocytosed from the basal plasma membrane and delivered to the RB. Delivery of membrane-associated transferrin to the RB further indicates that the RB is compositionally different from lysosomes and suggests that the endocytic pathway contributes to the maintenance of functional RB. In addition to transporting receptor-bound cargo to the RB, the endocytic pathway could act in balancing the membrane traffic associated with transcytosis from the RB to the basal plasma membrane. Endocytic processes (retrieval of mannose 6-phosphate receptors) in osteoclasts appeared to be fairly sensitive to bafilomycin A1, a specific inhibitor of vacuolar-type proton ATPases. Thus blocking the endocytic membrane traffic towards the RB could explain the inactivation of cells by low concentrations of the drug.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  K. K. Huynh, J. G. Kay, J. L. Stow, and S. Grinstein Fusion, Fission, and Secretion During Phagocytosis Physiology, December 1, 2007; 22(6): 366 - 372. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Czupalla, H. Mansukoski, T. Riedl, D. Thiel, E. Krause, and B. Hoflack Proteomic Analysis of Lysosomal Acid Hydrolases Secreted by Osteoclasts: Implications for Lytic Enzyme Transport and Bone Metabolism Mol. Cell. Proteomics, January 1, 2006; 5(1): 134 - 143. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Sun, K. G. Buki, O. Ettala, J. P. Vaaraniemi, and H. K. Vaananen Possible Role of Direct Rac1-Rab7 Interaction in Ruffled Border Formation of Osteoclasts J. Biol. Chem., September 16, 2005; 280(37): 32356 - 32361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Karsdal, K. Henriksen, M. G. Sorensen, J. Gram, S. Schaller, M. H. Dziegiel, A.-M. Heegaard, P. Christophersen, T. J. Martin, C. Christiansen, et al. Acidification of the Osteoclastic Resorption Compartment Provides Insight into the Coupling of Bone Formation to Bone Resorption Am. J. Pathol., February 1, 2005; 166(2): 467 - 476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Stenbeck and M. A. Horton Endocytic trafficking in actively resorbing osteoclasts J. Cell Sci., February 22, 2004; 117(6): 827 - 836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. P. Coxon, M. H. Helfrich, B. Larijani, M. Muzylak, J. E. Dunford, D. Marshall, A. D. McKinnon, S. A. Nesbitt, M. A. Horton, M. C. Seabra, et al. Identification of a Novel Phosphonocarboxylate Inhibitor of Rab Geranylgeranyl Transferase That Specifically Prevents Rab Prenylation in Osteoclasts and Macrophages J. Biol. Chem., December 14, 2001; 276(51): 48213 - 48222. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Parikka, P. Lehenkari, M.-L. Sassi, J. Halleen, J. Risteli, P. Harkonen, and H. K. Vaananen Estrogen Reduces the Depth of Resorption Pits by Disturbing the Organic Bone Matrix Degradation Activity of Mature Osteoclasts Endocrinology, December 1, 2001; 142(12): 5371 - 5378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhao, T. Laitala-Leinonen, V. Parikka, and H. K. Vaananen Downregulation of Small GTPase Rab7 Impairs Osteoclast Polarization and Bone Resorption J. Biol. Chem., October 12, 2001; 276(42): 39295 - 39302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G Stenbeck and M. Horton A new specialized cell-matrix interaction in actively resorbing osteoclasts J. Cell Sci., January 5, 2000; 113(9): 1577 - 1587. [Abstract] [PDF]
|
|
|
|
|
|
H. Vaananen, H Zhao, M Mulari, and J. Halleen The cell biology of osteoclast function J. Cell Sci., January 2, 2000; 113(3): 377 - 381. [Abstract] [PDF]
|
|
|
|
 |
|
 N. H. Battey, N. C. James, A. J. Greenland, and C. Brownlee Exocytosis and Endocytosis PLANT CELL, April 1, 1999; 11(4): 643 - 660. [Full Text]
|
|
|
|
|
|
T Laitala-Leinonen, C Lowik, S Papapoulos, and H. Vaananen Inhibition of intravacuolar acidification by antisense RNA decreases osteoclast differentiation and bone resorption in vitro J. Cell Sci., January 11, 1999; 112(21): 3657 - 3666. [Abstract] [PDF]
|
|
|
|
|
|
P Keller and K Simons Post-Golgi biosynthetic trafficking J. Cell Sci., January 12, 1997; 110(24): 3001 - 3009. [Abstract] [PDF]
|
|
|
|
 |
|
 J. G. Van Den Berg, J. Aten, C. Annink, J. H. Ravesloot, E. Weber, and J. J. Weening Interleukin-4 and -13 promote basolateral secretion of H+ and cathepsin L by glomerular epithelial cells Am J Physiol Renal Physiol, January 1, 2002; 282(1): F26 - F33. [Abstract] [Full Text] [PDF]
|
|
