spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Canman, J. C.
Right arrow Articles by Bement, W. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Canman, J. C.
Right arrow Articles by Bement, W. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 110, Issue 16 1907-1917, Copyright © 1997 by Company of Biologists

JOURNAL ARTICLES

Microtubules suppress actomyosin-based cortical flow in Xenopus oocytes

JC Canman and WM Bement
Department of Zoology, University of Wisconsin, Madison 53706, USA.

Several cell motility processes including cytokinesis and cell locomotion are dependent on the interplay of the microtubule and actomyosin cytoskeletons. However, because such processes are essentially visual phenomena, interactions between the two cytoskeletal systems have been difficult to study quantitatively. To overcome this difficulty, we have developed the Xenopus oocyte as an inducible, quantitative model system for actomyosin-based cortical flow and then exploited the strengths of this system to assess the relationship between microtubules and cortical flow. As in other systems, oocyte cortical flow entails: (1) redistribution of cortical filamentous actin (f-actin); (2) a requirement for actomyosin; (3) redistribution of cell surface proteins; (4) a requirement for cell surface protein mobility; and (5) directed movement of cortical organelles. Cortical flow rate in the oocyte system is inversely proportional to the level of polymeric tubulin and microinjection of free tubulin has no effect on the rate of cortical flow. Enhancement of microtubule polymerization inhibits cortical f-actin cable formation during cortical flow. The effects of microtubule depolymerization on cortical flow are rapid, independent of transcription or translation, independent of effects on the oocyte intermediate filament system, and independent of the upstream stimulus for cortical flow. The results show that the microtubules themselves, or a factor associated with them, suppress cortical flow, either by mechanically resisting flow, or by modulating the actomyosin cytoskeleton.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Genes Dev.Home page
M. Kwon, S. A. Godinho, N. S. Chandhok, N. J. Ganem, A. Azioune, M. Thery, and D. Pellman
Mechanisms to suppress multipolar divisions in cancer cells with extra centrosomes
Genes & Dev., August 15, 2008; 22(16): 2189 - 2203.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. I. Ivanov, A. M. Hopkins, G. T. Brown, K. Gerner-Smidt, B. A. Babbin, C. A. Parkos, and A. Nusrat
Myosin II regulates the shape of three-dimensional intestinal epithelial cysts
J. Cell Sci., June 1, 2008; 121(11): 1803 - 1814.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. V. Danilchik, E. E. Brown, and K. Riegert
Intrinsic chiral properties of the Xenopus egg cortex: an early indicator of left-right asymmetry?
Development, November 15, 2006; 133(22): 4517 - 4526.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
L. E. Urven, T. Yabe, and F. Pelegri
A role for non-muscle myosin II function in furrow maturation in the early zebrafish embryo
J. Cell Sci., October 15, 2006; 119(20): 4342 - 4352.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Lucero, C. Stack, A. R. Bresnick, and C. B. Shuster
A Global, Myosin Light Chain Kinase-dependent Increase in Myosin II Contractility Accompanies the Metaphase-Anaphase Transition in Sea Urchin Eggs
Mol. Biol. Cell, September 1, 2006; 17(9): 4093 - 4104.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
H. A. Benink and W. M. Bement
Concentric zones of active RhoA and Cdc42 around single cell wounds
J. Cell Biol., January 31, 2005; 168(3): 429 - 439.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
K. L. Weber and W. M. Bement
F-actin serves as a template for cytokeratin organization in cell free extracts
J. Cell Sci., January 4, 2002; 115(7): 1373 - 1382.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
N. Kabir, A. W. Schaefer, A. Nakhost, W. S. Sossin, and P. Forscher
Protein Kinase C Activation Promotes Microtubule Advance in Neuronal Growth Cones by Increasing Average Microtubule Growth Lifetimes
J. Cell Biol., March 5, 2001; 152(5): 1033 - 1044.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
T. Wittmann and C. M. Waterman-Storer
Cell motility: can Rho GTPases and microtubules point the way?
J. Cell Sci., January 11, 2001; 114(21): 3795 - 3803.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
H. A. Benink, C. A. Mandato, and W. M. Bement
Analysis of Cortical Flow Models In Vivo
Mol. Biol. Cell, August 1, 2000; 11(8): 2553 - 2563.
[Abstract] [Full Text]

Home page
 JCBHome page
C. Waterman-Storer, D. Y. Duey, K. L. Weber, J. Keech, R. E. Cheney, E.D. Salmon, and W. M. Bement
Microtubules Remodel Actomyosin Networks in Xenopus Egg Extracts via Two Mechanisms of F-Actin Transport
J. Cell Biol., July 24, 2000; 150(2): 361 - 376.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. Silverman-Gavrila and A Forer
Evidence that actin and myosin are involved in the poleward flux of tubulin in metaphase kinetochore microtubules of crane-fly spermatocytes
J. Cell Sci., January 2, 2000; 113(4): 597 - 609.
[Abstract] [PDF]

Home page
 Mol. Biol. CellHome page
D. M. Helfman, E. T. Levy, C. Berthier, M. Shtutman, D. Riveline, I. Grosheva, A. Lachish-Zalait, M. Elbaum, and A. D. Bershadsky
Caldesmon Inhibits Nonmuscle Cell Contractility and Interferes with the Formation of Focal Adhesions
Mol. Biol. Cell, October 1, 1999; 10(10): 3097 - 3112.
[Abstract] [Full Text]

Home page
 J. Neurosci.Home page
E. A. Welnhofer, L. Zhao, and C. S. Cohan
Calcium Influx Alters Actin Bundle Dynamics and Retrograde Flow in Helisoma Growth Cones
J. Neurosci., September 15, 1999; 19(18): 7971 - 7982.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. Odde, L Ma, A. Briggs, A DeMarco, and M. Kirschner
Microtubule bending and breaking in living fibroblast cells
J. Cell Sci., January 10, 1999; 112(19): 3283 - 3288.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
J. Sider, C. Mandato, K. Weber, A. Zandy, D Beach, R. Finst, J Skoble, and W. Bement
Direct observation of microtubule-f-actin interaction in cell free lysates
J. Cell Sci., January 6, 1999; 112(12): 1947 - 1956.
[Abstract] [PDF]

Home page
 JCBHome page
C. A. Mandato and W. M. Bement
Contraction and polymerization cooperate to assemble and close actomyosin rings around Xenopus oocyte wounds
J. Cell Biol., August 20, 2001; 154(4): 785 - 798.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. Stamenovic, S. M. Mijailovich, I. M. Tolic-Norrelykke, J. Chen, and N. Wang
Cell prestress. II. Contribution of microtubules
Am J Physiol Cell Physiol, March 1, 2002; 282(3): C617 - C624.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1997