Journal of Cell Science, Vol 110, Issue 17 2079-2087, Copyright © 1997 by Company of Biologists

JOURNAL ARTICLES

NSF is required for transport from early to late endosomes

LJ Robinson, F Aniento and J Gruenberg
Department of Biochemistry, University of Geneva, Switzerland.

Protein transport between early and late endosomes is a major membrane trafficking pathway in the cell followed by many proteins, including all down-regulated receptors. Yet, little is known at the molecular level about the mechanisms regulating membrane interactions in the endocytic pathway beyond early endosomes. In this study, we have used an in vitro transport assay to study the biochemical properties of endosome docking/fusion events. Our data demonstrate that N-ethylmaleimide (NEM) sensitive factor (NSF) and its soluble associated proteins (SNAPs) are required for transport from early to late endosomes, as well as at all other steps of endosomal membrane transport. We also find that these proteins are enriched on endosomal membranes. In addition, our studies suggest that besides NSF/SNAPs, another NEM-sensitive component may also be involved in docking/fusion at this late stage of the pathway. Finally, we find that, in contrast to Golgi membranes, NSF association to both early and late endosomal membranes occurs via an ATP-independent mechanism, indicating that the binding properties of endosomal and biosynthetic NSF are different. Our data thus show that NSF/SNAPs, perhaps together with another NEM-sensitive factor, are part of the basic molecular machinery which controls docking/fusion events during transport from early to late endosomes, along the lysosomal degradation pathway.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				F. Balzac, M. Avolio, S. Degani, I. Kaverina, M. Torti, L. Silengo, J. V. Small, and S. F. Retta E-cadherin endocytosis regulates the activity of Rap1: a traffic light GTPase at the crossroads between cadherin and integrin function J. Cell Sci., October 15, 2005; 118(20): 4765 - 4783. [Abstract] [Full Text] [PDF]

				W. Antonin, C. Holroyd, R. Tikkanen, S. Höning, and R. Jahn The R-SNARE Endobrevin/VAMP-8 Mediates Homotypic Fusion of Early Endosomes and Late Endosomes Mol. Biol. Cell, October 1, 2000; 11(10): 3289 - 3298. [Abstract] [Full Text]

				R. Gagescu, N. Demaurex, R. G. Parton, W. Hunziker, L. A. Huber, and J. Gruenberg The Recycling Endosome of Madin-Darby Canine Kidney Cells Is a Mildly Acidic Compartment Rich in Raft Components Mol. Biol. Cell, August 1, 2000; 11(8): 2775 - 2791. [Abstract] [Full Text]

				F. Gu and J. Gruenberg ARF1 Regulates pH-dependent COP Functions in the Early Endocytic Pathway J. Biol. Chem., March 10, 2000; 275(11): 8154 - 8160. [Abstract] [Full Text] [PDF]

				K. Mukherjee, S. A. Siddiqi, S. Hashim, M. Raje, S. K. Basu, and A. Mukhopadhyay Live Salmonella Recruits N-Ethylmaleimide-Sensitive Fusion Protein on Phagosomal Membrane and Promotes Fusion with Early Endosome J. Cell Biol., February 21, 2000; 148(4): 741 - 754. [Abstract] [Full Text] [PDF]

				M Ohashi, I Miwako, A Yamamoto, and K Nagayama Arrested maturing multivesicular endosomes observed in a Chinese hamster ovary cell mutant, LEX2, isolated by repeated flow-cytometric cell sorting J. Cell Sci., January 6, 2000; 113(12): 2187 - 2205. [Abstract] [PDF]

				S. Urbe, L. J. Page, and S. A. Tooze Homotypic Fusion of Immature Secretory Granules during Maturation in a Cell-free Assay J. Cell Biol., December 28, 1998; 143(7): 1831 - 1844. [Abstract] [Full Text] [PDF]

				B. L. Tang, A. E. H. Tan, L. K. Lim, S. S. Lee, D. Y. H. Low, and W. Hong Syntaxin 12, a Member of the Syntaxin Family Localized to the Endosome J. Biol. Chem., March 20, 1998; 273(12): 6944 - 6950. [Abstract] [Full Text] [PDF]