|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 110, Issue 18 2261-2270, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
S Tavella, G Bellese, P Castagnola, I Martin, D Piccini, R Doliana, A Colombatti, R Cancedda and C Tacchetti
Istituto Nazionale per la Ricerca sul Cancro, Centro Biotecnologie Avanzate, Genova, Italy.
We have investigated the expression and localization of fibronectin, laminin, and their receptors, and we used an in vitro chick chondrocyte differentiation model to define a time hierarchy for their appearance in early chondro-genesis and to determine their role in the cell condensation process. By serum fibronectin depletion/reconstitution, or GRGDSP peptide competition experiments, we show that fibronectin contributes to the initial cell-cell interactions that occur during condensation. In later stages, a down-regulation of both fibronectin and of its alpha5beta1 integrin receptor occur, as demonstrated by mRNA and protein kinetics. Immunolocalisation studies suggest that the reduction of fibronectin in discrete areas is involved in local activation of the cell differentiation program. Furthermore, we show that laminin is expressed during the in vitro cell condensation process in areas that are negative for fibronectin staining. The types of laminin as well as the timing of expression have been determined by northern blot and RT-PCR analyses. The highest levels of expression are coincident with maximal cell aggregation. The alpha3beta1 laminin receptor, highly expressed in dedifferentiated cells, follows later on the ligand trend. During in vitro chondrogenesis, a down-regulation in the B isoform, and an up-regulation of the A isoform, of the alpha subunit of the alpha6beta1 laminin receptor occurs. Immunolocalisation studies suggest that laminin is involved in the definition of differentiating areas as opposed to non differentiating areas of the condensed region, i.e. the periphery, which eventually gives rise to the perichondrium.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  A. M. Kloxin, A. M. Kasko, C. N. Salinas, and K. S. Anseth Photodegradable Hydrogels for Dynamic Tuning of Physical and Chemical Properties Science, April 3, 2009; 324(5923): 59 - 63. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Gigout, M. Jolicoeur, M. Nelea, N. Raynal, R. Farndale, and M. D. Buschmann Chondrocyte Aggregation in Suspension Culture Is GFOGER-GPP- and {beta}1 Integrin-dependent J. Biol. Chem., November 14, 2008; 283(46): 31522 - 31530. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. L. Galante and J. E. Schwarzbauer Requirements for sulfate transport and the diastrophic dysplasia sulfate transporter in fibronectin matrix assembly J. Cell Biol., December 3, 2007; 179(5): 999 - 1009. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Behera, E. Hildebrand, S. Uematsu, S. Akira, J. Coburn, and L. T. Hu Identification of a TLR-Independent Pathway for Borrelia burgdorferi-Induced Expression of Matrix Metalloproteinases and Inflammatory Mediators through Binding to Integrin {alpha}3beta1 J. Immunol., July 1, 2006; 177(1): 657 - 664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Segat, R. Comai, E. Di Marco, A. Strangio, R. Cancedda, A. T. Franzi, and C. Tacchetti Integrins alpha 6Abeta 1 and alpha 6Bbeta 1 Promote Different Stages of Chondrogenic Cell Differentiation J. Biol. Chem., August 23, 2002; 277(35): 31612 - 31622. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-M. Yoon, S.-J. Kim, C.-D. Oh, J.-W. Ju, W. K. Song, Y. J. Yoo, T.-L. Huh, and J.-S. Chun Maintenance of Differentiated Phenotype of Articular Chondrocytes by Protein Kinase C and Extracellular Signal-regulated Protein Kinase J. Biol. Chem., March 1, 2002; 277(10): 8412 - 8420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-M. Yoon, C.-D. Oh, D.-Y. Kim, Y.-S. Lee, J.-W. Park, T.-L. Huh, S.-S. Kang, and J.-S. Chun Epidermal Growth Factor Negatively Regulates Chondrogenesis of Mesenchymal Cells by Modulating the Protein Kinase C-alpha , Erk-1, and p38 MAPK Signaling Pathways J. Biol. Chem., April 14, 2000; 275(16): 12353 - 12359. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-D. Oh, S.-H. Chang, Y.-M. Yoon, S.-J. Lee, Y.-S. Lee, S.-S. Kang, and J.-S. Chun Opposing Role of Mitogen-activated Protein Kinase Subtypes, Erk-1/2 and p38, in the Regulation of Chondrogenesis of Mesenchymes J. Biol. Chem., February 25, 2000; 275(8): 5613 - 5619. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Chang, C.-D. Oh, M.-S. Yang, S.-S. Kang, Y.-S. Lee, J.-K. Sonn, and J.-S. Chun Protein Kinase C Regulates Chondrogenesis of Mesenchymes via Mitogen-activated Protein Kinase Signaling J. Biol. Chem., July 24, 1998; 273(30): 19213 - 19219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. K. Panda, D. Miao, V. Lefebvre, G. N. Hendy, and D. Goltzman The Transcription Factor SOX9 Regulates Cell Cycle and Differentiation Genes in Chondrocytic CFK2 Cells J. Biol. Chem., October 26, 2001; 276(44): 41229 - 41236. [Abstract] [Full Text] [PDF]
|
|
