|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 110, Issue 19 2333-2344, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
H Aizawa, Y Fukui and I Yahara
Department of Cell Biology, The Tokyo Metropolitan Institute of Medical Science, Japan.
Cofilin, an indispensable, actin-regulating protein represents the 'cofilin family' of actin-binding proteins existing in a wide variety of organisms. Our previous and other in vitro studies have implied that cofilin can accelerate transformation of filamentous (F)-actin and (alpha)-actinin latticework into bundles, and overexpression of cofilin induces formation of F-actin bundles in Dictyostelium. Here we expressed an Aequorea green fluorescent protein (GFP)-Dictyostelium cofilin fusion protein in Dictyostelium, and observed the live dynamics to examine the physiological function of cofilin. We show that purified GFP-cofilin binds to actin filaments and decreases the apparent viscosity of actin solution in a similar manner to authentic Dictyostelium cofilin. Expressed GFP-cofilin exhibits normal actin-binding activities in the cytoplasm as represented by incorporation into the actin rods induced with dimethyl sulfoxide. Free moving cells form a crown-like cortical structure on the dorsal surface, and GFP-cofilin exhibits dynamic assembly into actin bundles being formed beneath the cortex. During phagocytosis, GFP-cofilin accumulates into actin bundles formed in the region underlying the phagocytic cups. In cells chemotactically activated with cyclic AMP, GFP-cofilin exhibits a high level of accumulation in projecting leading edges. When the chemo-attraction is experimentally changed, the redistribution of GFP-cofilin towards the new pseudopod occurs in a matter of 30-60 seconds. These results demonstrate that cofilin plays a crucial role in vivo in rapid remodeling of the cortical actin meshwork into bundles.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  X.-B. Li, D. Xu, X.-L. Wang, G.-Q. Huang, J. Luo, D.-D. Li, Z.-T. Zhang, and W.-L. Xu Three cotton genes preferentially expressed in flower tissues encode actin-depolymerizing factors which are involved in F-actin dynamics in cells J. Exp. Bot., October 27, 2009; (2009) erp280v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Dewitt, R. L. Darley, and M. B. Hallett Translocation or just location? Pseudopodia affect fluorescent signals J. Cell Biol., January 26, 2009; 184(2): 197 - 203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Kolsch, P. G. Charest, and R. A. Firtel The regulation of cell motility and chemotaxis by phospholipid signaling J. Cell Sci., March 1, 2008; 121(5): 551 - 559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Champion and S. Mitragotri From the Cover: Role of target geometry in phagocytosis PNAS, March 28, 2006; 103(13): 4930 - 4934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. H. Lin, Z.-Y. Park, D. Lin, A. A. Brahmbhatt, M.-C. Rio, J. R. Yates III, and R. L. Klemke Regulation of cell migration and survival by focal adhesion targeting of Lasp-1 J. Cell Biol., May 10, 2004; 165(3): 421 - 432. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. N. Steenbergen, J. D. Nosanchuk, S. D. Malliaris, and A. Casadevall Cryptococcus neoformans Virulence Is Enhanced after Growth in the Genetically Malleable Host Dictyostelium discoideum Infect. Immun., September 1, 2003; 71(9): 4862 - 4872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Garcia-Garcia and C. Rosales Signal transduction during Fc receptor-mediated phagocytosis J. Leukoc. Biol., December 1, 2002; 72(6): 1092 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Rubin and S. Ravid Polarization of Myosin II Heavy Chain-Protein Kinase C in Chemotaxing Dictyostelium Cells J. Biol. Chem., September 20, 2002; 277(39): 36005 - 36008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Y. Chen, E. I. Wong, L. Vidali, A. Estavillo, P. K. Hepler, H.-m. Wu, and A. Y. Cheung The Regulation of Actin Organization by Actin-Depolymerizing Factor in Elongating Pollen Tubes PLANT CELL, September 1, 2002; 14(9): 2175 - 2190. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Watanabe, M. Ichihara, M. Hashimoto, K. Shimono, Y. Shimoyama, T. Nagasaka, Y. Murakumo, H. Murakami, H. Sugiura, H. Iwata, et al. Characterization of Gene Expression Induced by RET with MEN2A or MEN2B Mutation Am. J. Pathol., July 1, 2002; 161(1): 249 - 256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Maselli, G. Laevsky, and D. A. Knecht Kinetics of binding, uptake and degradation of live fluorescent (DsRed) bacteria by Dictyostelium discoideum Microbiology, February 1, 2002; 148(2): 413 - 420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Dong, G.-X. Xia, Y. Hong, S. Ramachandran, B. Kost, and N.-H. Chua ADF Proteins Are Involved in the Control of Flowering and Regulate F-Actin Organization, Cell Expansion, and Organ Growth in Arabidopsis PLANT CELL, June 1, 2001; 13(6): 1333 - 1346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V.-M. Loitto, B. Rasmusson, and K.-E. Magnusson Assessment of neutrophil N-formyl peptide receptors by using antibodies and fluorescent peptides J. Leukoc. Biol., May 1, 2001; 69(5): 762 - 771. [Abstract] [Full Text]
|
|
|
|
|
|
R. May and L. Machesky Phagocytosis and the actin cytoskeleton J. Cell Sci., January 3, 2001; 114(6): 1061 - 1077. [Abstract] [PDF]
|
|
|
|
|
|
D. N. Robinson and J. A. Spudich Dynacortin, a Genetic Link between Equatorial Contractility and Global Shape Control Discovered by Library Complementation of a Dictyostelium discoideum Cytokinesis Mutant J. Cell Biol., August 21, 2000; 150(4): 823 - 838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Noegel and M Schleicher The actin cytoskeleton of Dictyostelium: a story told by mutants J. Cell Sci., January 3, 2000; 113(5): 759 - 766. [Abstract] [PDF]
|
|
|
|
|
|
A. Konzok, I. Weber, E. Simmeth, U. Hacker, M. Maniak, and A. Muller-Taubenberger Daip1, a Dictyostelium Homologue of the Yeast Actin-Interacting Protein 1, Is Involved in Endocytosis, Cytokinesis, and Motility J. Cell Biol., July 26, 1999; 146(2): 453 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Parent and P. N. Devreotes A Cell's Sense of Direction Science, April 30, 1999; 284(5415): 765 - 770. [Abstract] [Full Text]
|
|
|
|
|
|
M. Bailly, F. Macaluso, M. Cammer, A. Chan, J. E. Segall, and J. S. Condeelis Relationship between Arp2/3 Complex and the Barbed Ends of Actin Filaments at the Leading Edge of Carcinoma Cells after Epidermal Growth Factor Stimulation J. Cell Biol., April 19, 1999; 145(2): 331 - 345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S Yumura and Y Fukui Spatiotemporal dynamics of actin concentration during cytokinesis and locomotion in Dictyostelium J. Cell Sci., January 8, 1998; 111(15): 2097 - 2108. [Abstract] [PDF]
|
|
|
|
|
|
H. Bierne, E. Gouin, P. Roux, P. Caroni, H. L. Yin, and P. Cossart A role for cofilin and LIM kinase in Listeria-induced phagocytosis J. Cell Biol., October 1, 2001; 155(1): 101 - 112. [Abstract] [Full Text] [PDF]
|
|
