spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Subtil, A.
Right arrow Articles by Dautry-Varsat, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Subtil, A.
Right arrow Articles by Dautry-Varsat, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal of Cell Science, Vol 110, Issue 19 2441-2447, Copyright © 1997 by Company of Biologists

JOURNAL ARTICLES

Microtubule depolymerization inhibits clathrin coated-pit internalization in non-adherent cell lines while interleukin 2 endocytosis is not affected

A Subtil and A Dautry-Varsat
Unite de Biologie des Interactions Cellulaires, URA CNRS 1960, Institut Pasteur, Paris, France.

The microtubule cytoskeleton is generally not considered to be essential for the first steps of clathrin-mediated endocytosis of membrane receptors. Its role in clathrin-independent endocytosis has not been investigated. We have previously shown that the cytokine interleukin 2 (IL2) is internalized in lymphoid cells expressing its receptors when clathrin-dependent endocytosis is inhibited. Here we compare the internalization of IL2 and of transferrin, a marker of clathrin-dependent endocytosis, after microtubule disruption. In hemopoietic cell lines, which express IL2 receptors, transferrin receptor entry was inhibited by about 40%. However, in adherent cell lines, transferrin entry was unaffected by microtubule disruption, as previously reported. Unlike the case for transferrin, internalization of IL2 receptors was not affected by depolymerization of the microtubule cytoskeleton in hemopoietic cell lines. These results show that IL2 and transferrin receptors do not have the same endocytic properties and support our previous conclusion that these receptors follow different pathways of endocytosis.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
B. Wu, B. Decourt, M. A. Zabidi, L. T. Wuethrich, W. H. Kim, Z. Zhou, K. MacIsaac, and D. M. Suter
Microtubule-mediated Src Tyrosine Kinase Trafficking in Neuronal Growth Cones
Mol. Biol. Cell, November 1, 2008; 19(11): 4611 - 4627.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Uezu, A. Horiuchi, K. Kanda, N. Kikuchi, K. Umeda, K. Tsujita, S. Suetsugu, N. Araki, H. Yamamoto, T. Takenawa, et al.
SGIP1{alpha} Is an Endocytic Protein That Directly Interacts with Phospholipids and Eps15
J. Biol. Chem., September 7, 2007; 282(36): 26481 - 26489.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Zhao, I. Gaidarov, and J. H. Keen
Phosphoinositide 3-Kinase C2{alpha} Links Clathrin to Microtubule-dependent Movement
J. Biol. Chem., January 12, 2007; 282(2): 1249 - 1256.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A.-S. Zhang, A. D. Sheftel, and P. Ponka
Intracellular kinetics of iron in reticulocytes: evidence for endosome involvement in iron targeting to mitochondria
Blood, January 1, 2005; 105(1): 368 - 375.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F. Baluska, J. Samaj, A. Hlavacka, J. Kendrick-Jones, and D. Volkmann
Actin-dependent fluid-phase endocytosis in inner cortex cells of maize root apices
J. Exp. Bot., February 1, 2004; 55(396): 463 - 473.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. Shigematsu, A. H. Khan, M. Kanzaki, and J. E. Pessin
Intracellular Insulin-Responsive Glucose Transporter (GLUT4) Distribution But Not Insulin-Stimulated GLUT4 Exocytosis and Recycling Are Microtubule Dependent
Mol. Endocrinol., May 1, 2002; 16(5): 1060 - 1068.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. L. Torgersen, G. Skretting, B. van Deurs, and K. Sandvig
Internalization of cholera toxin by different endocytic mechanisms
J. Cell Sci., March 12, 2002; 114(20): 3737 - 3747.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Zaslaver, R. Feniger-Barish, and A. Ben-Baruch
Actin Filaments Are Involved in the Regulation of Trafficking of Two Closely Related Chemokine Receptors, CXCR1 and CXCR2
J. Immunol., January 15, 2001; 166(2): 1272 - 1284.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. Yu, F. Olosz, C. Y. Choi, and T. R. Malek
Efficient Internalization of IL-2 Depends on the Distal Portion of the Cytoplasmic Tail of the IL-2R Common {gamma}-Chain and a Lymphoid Cell Environment
J. Immunol., September 1, 2000; 165(5): 2556 - 2562.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
G Skretting, M. Torgersen, B van Deurs, and K Sandvig
Endocytic mechanisms responsible for uptake of GPI-linked diphtheria toxin receptor
J. Cell Sci., January 11, 1999; 112(22): 3899 - 3909.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
D. L. Silver, N. Wang, X. Xiao, and A. R. Tall
High Density Lipoprotein (HDL) Particle Uptake Mediated by Scavenger Receptor Class B Type 1 Results in Selective Sorting of HDL Cholesterol from Protein and Polarized Cholesterol Secretion
J. Biol. Chem., June 29, 2001; 276(27): 25287 - 25293.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 1997