|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 110, Issue 2 179-189, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
T Asada, R Kuriyama and H Shibaoka
Department of Biology, Graduate School of Science, Osaka University, Japan.
Analysis of a cDNA for a 125 kDa polypeptide, previously isolated from phragmoplasts of tobacco BY-2 cells as a candidate for a plus end-directed microtubule motor, revealed this polypeptide to be a novel member of the kinesin superfamily. We named this protein TKRP125 (tobacco kinesin-related polypeptide of 125 kDa). The strong similarity between TKRP125 and members of the bimC subfamily in terms of the amino acid sequence of the amino-terminal motor domain indicated that TKRP125 belonged to the bimC subfamily. An antibody against a short peptide from the motor domain of TKRP125 inhibited the GTP- or ATP-dependent translocation of phragmoplast microtubules in membrane-permeabilized BY-2 cells, suggesting a role for TKRP125 in microtubule translocation, which is considered to be involved in the formation and/or maintenance of the bipolar structure of the phragmoplast. The expression of TKRP125 was found to be cell cycle-dependent. TKRP125 was not present in cells at the G1 phase. It began to appear at the S phase and accumulated during the G2 phase. The distribution of TKRP125 changed as the arrangement of microtubules changed with the progression of the cell cycle. TKRP125 was distributed along cortical microtubules during the S phase and along microtubules in the preprophase band and perinuclear microtubules in premitotic cells. It was also present in the nucleus in premitotic cells. In cells in M phase, TKRP125 was distributed along spindle microtubules. It accumulated at the equatorial plane of the spindle as the spindle elongated. In cytokinetic cells, TKRP125 was colocalized with phragmoplast microtubules. These observations suggest the possible involvement of TKRP125 in the cell cycle-dependent changes in arrays of microtubules, including the organization of the phragmoplast, and in the movement of chromosomes in anaphase cells.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Buschmann and C. W. Lloyd Arabidopsis Mutants and the Network of Microtubule-Associated Functions Mol Plant, November 1, 2008; 1(6): 888 - 898. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Segui-Simarro, M. J. Coronado, and L. A. Staehelin The Mitochondrial Cycle of Arabidopsis Shoot Apical Meristem and Leaf Primordium Meristematic Cells Is Defined by a Perinuclear Tentaculate/Cage-Like Mitochondrion Plant Physiology, November 1, 2008; 148(3): 1380 - 1393. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-C. Caillaud, P. Lecomte, F. Jammes, M. Quentin, S. Pagnotta, E. Andrio, J. de Almeida Engler, N. Marfaing, P. Gounon, P. Abad, et al. MAP65-3 Microtubule-Associated Protein Is Essential for Nematode-Induced Giant Cell Ontogenesis in Arabidopsis PLANT CELL, February 1, 2008; 20(2): 423 - 437. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bannigan, W.-R. Scheible, W. Lukowitz, C. Fagerstrom, P. Wadsworth, C. Somerville, and T. I. Baskin A conserved role for kinesin-5 in plant mitosis J. Cell Sci., August 15, 2007; 120(16): 2819 - 2827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-R. J. Lee, Y. Li, and B. Liu Two Arabidopsis Phragmoplast-Associated Kinesins Play a Critical Role in Cytokinesis during Male Gametogenesis PLANT CELL, August 1, 2007; 19(8): 2595 - 2605. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Goto and T. Asada Excessive Expression of the Plant Kinesin TBK5 Converts Cortical and Perinuclear Microtubules into a Radial Array Emanating From a Single Focus Plant Cell Physiol., May 1, 2007; 48(5): 753 - 761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. R. Hildebrandt, L. Gheber, T. Kingsbury, and M. A. Hoyt Homotetrameric Form of Cin8p, a Saccharomyces cerevisiae Kinesin-5 Motor, Is Essential for Its in Vivo Function J. Biol. Chem., September 8, 2006; 281(36): 26004 - 26013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Umeki, T. Mitsui, N. Umezu, K. Kondo, and S. Maruta Preparation and characterization of a novel rice plant-specific Kinesin. J. Biochem., April 1, 2006; 139(4): 645 - 654. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Abdel-Ghany, I. S. Day, M. P. Simmons, P. Kugrens, and A. S.N. Reddy Origin and Evolution of Kinesin-Like Calmodulin-Binding Protein Plant Physiology, July 1, 2005; 138(3): 1711 - 1722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Oka, Y. Yanagawa, T. Asada, A. Yoneda, S. Hasezawa, T. Sato, and H. Nakagawa Inhibition of Proteasome by MG-132 Treatment Causes Extra Phragmoplast Formation and Cortical Microtubule Disorganization during M/G1 Transition in Synchronized Tobacco Cells Plant Cell Physiol., November 15, 2004; 45(11): 1623 - 1632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Vanstraelen, J. A. Torres Acosta, L. De Veylder, D. Inze, and D. Geelen A Plant-Specific Subclass of C-Terminal Kinesins Contains a Conserved A-Type Cyclin-Dependent Kinase Site Implicated in Folding and Dimerization Plant Physiology, July 1, 2004; 135(3): 1417 - 1429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Yoneda, M. Akatsuka, F. Kumagai, and S. Hasezawa Disruption of Actin Microfilaments Causes Cortical Microtubule Disorganization and Extra-Phragmoplast Formation at M/G1 Interface in Synchronized Tobacco Cells Plant Cell Physiol., June 15, 2004; 45(6): 761 - 769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zhong, D. H. Burk, W. H. Morrison III, and Z.-H. Ye A Kinesin-Like Protein Is Essential for Oriented Deposition of Cellulose Microfibrils and Cell Wall Strength PLANT CELL, December 1, 2002; 14(12): 3101 - 3117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yasuhara, M. Muraoka, H. Shogaki, H. Mori, and S. Sonobe TMBP200, a Microtubule Bundling Polypeptide Isolated from Telophase Tobacco BY-2 Cells is a MOR1 Homologue Plant Cell Physiol., June 15, 2002; 43(6): 595 - 603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. N.V. Geelen and D. G. Inze A Bright Future for the Bright Yellow-2 Cell Culture Plant Physiology, December 1, 2001; 127(4): 1375 - 1379. [Full Text] [PDF]
|
|
|
|
|
|
Y.-R. J. Lee, H. M. Giang, and B. Liu A Novel Plant Kinesin-Related Protein Specifically Associates with the Phragmoplast Organelles PLANT CELL, November 1, 2001; 13(11): 2427 - 2439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Otegui, D. N. Mastronarde, B.-H. Kang, S. Y. Bednarek, and L. A. Staehelin Three-Dimensional Analysis of Syncytial-Type Cell Plates during Endosperm Cellularization Visualized by High Resolution Electron Tomography PLANT CELL, September 1, 2001; 13(9): 2033 - 2051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Weingartner, P. Binarova, D. Drykova, A. Schweighofer, J.-P. David, E. Heberle-Bors, J. Doonan, and L. Bogre Dynamic Recruitment of Cdc2 to Specific Microtubule Structures during Mitosis PLANT CELL, August 1, 2001; 13(8): 1929 - 1943. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Nishihama, M. Ishikawa, S. Araki, T. Soyano, T. Asada, and Y. Machida The NPK1 mitogen-activated protein kinase kinase kinase is a regulator of cell-plate formation in plant cytokinesis Genes & Dev., February 1, 2001; 15(3): 352 - 363. [Abstract] [Full Text]
|
|
|
|
|
|
S. Sonobe, S. Yamamoto, M. Motomura, and T. Shimmen Isolation of Cortical MTs from Tobacco BY-2 Cells Plant Cell Physiol., February 1, 2001; 42(2): 162 - 169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I Touitou, G Lhomond, and G Pruliere Boursin, a sea urchin bimC kinesin protein, plays a role in anaphase and cytokinesis J. Cell Sci., January 2, 2001; 114(3): 481 - 491. [Abstract] [PDF]
|
|
|
|
|
|
G. Cai, S. Romagnoli, A. Moscatelli, E. Ovidi, G. Gambellini, A. Tiezzi, and M. Cresti Identification and Characterization of a Novel Microtubule-Based Motor Associated with Membranous Organelles in Tobacco Pollen Tubes PLANT CELL, September 1, 2000; 12(9): 1719 - 1736. [Abstract] [Full Text]
|
|
|
|
|
|
J. Zuo, Q.-W. Niu, N. Nishizawa, Y. Wu, B. Kost, and N.-H. Chua KORRIGAN, an Arabidopsis Endo-1,4-{beta}-Glucanase, Localizes to the Cell Plate by Polarized Targeting and Is Essential for Cytokinesis PLANT CELL, July 1, 2000; 12(7): 1137 - 1152. [Abstract] [Full Text]
|
|
|
|
|
|
J. W. Vos, F. Safadi, A. S. N. Reddy, and P. K. Hepler The Kinesin-like Calmodulin Binding Protein Is Differentially Involved in Cell Division PLANT CELL, June 1, 2000; 12(6): 979 - 990. [Abstract] [Full Text]
|
|
|
|
|
|
L. Bögre, O. Calderini, P. Binarova, M. Mattauch, S. Till, S. Kiegerl, C. Jonak, C. Pollaschek, P. Barker, N. S. Huskisson, et al. A MAP Kinase Is Activated Late in Plant Mitosis and Becomes Localized to the Plane of Cell Division PLANT CELL, January 1, 1999; 11(1): 101 - 114. [Abstract] [Full Text]
|
|
|
|
|
|
S. B. Narasimhulu and A. S. N. Reddy Characterization of Microtubule Binding Domains in the Arabidopsis Kinesin-like Calmodulin Binding Protein PLANT CELL, June 1, 1998; 10(6): 957 - 966. [Abstract] [Full Text]
|
|
|
|
|
|
O Calderini, L Bogre, O Vicente, P Binarova, E Heberle-Bors, and C Wilson A cell cycle regulated MAP kinase with a possible role in cytokinesis in tobacco cells J. Cell Sci., January 10, 1998; 111(20): 3091 - 3100. [Abstract] [PDF]
|
|
|
|
|
|
D. Drummond and I. Hagan Mutations in the bimC box of Cut7 indicate divergence of regulation within the bimC family of kinesin related proteins J. Cell Sci., January 4, 1998; 111(7): 853 - 865. [Abstract] [PDF]
|
|
|
|
 |
|
 M. H. Lauber, I. Waizenegger, T. Steinmann, H. Schwarz, U. Mayer, I. Hwang, W. Lukowitz, and G. Jurgens The Arabidopsis KNOLLE Protein Is a Cytokinesis-specific Syntaxin J. Cell Biol., December 15, 1997; 139(6): 1485 - 1493. [Abstract] [Full Text] [PDF]
|
|
