|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 110, Issue 2 239-247, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
MJ Lallena and I Correas
Centro de Biologia Molecular Severo Ochoa (CSIC-UAM), Universidad Autonoma de Madrid, Spain.
Protein 4.1, originally identified as a component of the membrane-skeleton of the red blood cell, has also been localized in the nucleus of mammalian cells. To learn more about nuclear 4.1 protein, we have analyzed the nature of its association with the nuclear structure in comparison with SC35 and snRNP antigens, splicing proteins of the nuclear speckle domains. When MDCK or HeLa cells were digested with DNase I and washed in the presence of high salt (2 M NaCl), snRNP antigens were extracted whereas protein 4.1 and SC35 remained colocalizing in nuclear speckles. In cells treated with RNase A or heat shocked, nuclear 4.1 distribution also resembled that of SC35. Experiments carried out in transcriptionally active nuclei showed that protein 4.1 distributed in irregularly shaped speckles which appeared to be interconnected. During transcriptional inhibition, protein 4.1 accumulated in rounded speckles lacking interconnections. When cells were released from transcriptional inhibition, protein 4.1 redistributed back to the interconnected speckle pattern of transcriptionally active cells, as it was also observed for SC35. Finally, coprecipitation of 4.1 and SC35 proteins from RNase A digested HeLa nuclei further indicates that these two proteins are associated, forming part of the nuclear speckle domains to which they attach more tightly than snRNP antigens.
This article has been cited by other articles:
|
|
 |
|
  S.-C. Huang, E. S. Liu, S.-H. Chan, I. D. Munagala, H. T. Cho, R. Jagadeeswaran, and E. J. Benz Jr Mitotic Regulation of Protein 4.1R Involves Phosphorylation by cdc2 Kinase Mol. Biol. Cell, January 1, 2005; 16(1): 117 - 127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Perez-Ferreiro, I. Vernos, and I. Correas Protein 4.1R regulates interphase microtubule organization at the centrosome J. Cell Sci., December 1, 2004; 117(25): 6197 - 6206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-C. Huang, R. Jagadeeswaran, E. S. Liu, and E. J. Benz Jr. Protein 4.1R, a Microtubule-associated Protein Involved in Microtubule Aster Assembly in Mammalian Mitotic Extract J. Biol. Chem., August 13, 2004; 279(33): 34595 - 34602. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Zimowska, J. Shi, G. Munguba, M. R. Jackson, R. Alpatov, M. N. Simmons, Y. Shi, and S. P. Sugrue Pinin/DRS/memA Interacts with SRp75, SRm300 and SRrp130 in Corneal Epithelial Cells Invest. Ophthalmol. Vis. Sci., November 1, 2003; 44(11): 4715 - 4723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. DeSousa, M. Mukhopadhyay, P. Pelka, X. Zhao, B. K. Dey, V. Robert, A. Pelisson, A. Bucheton, and A. R. Campos A Novel Double-stranded RNA-binding Protein, Disco Interacting Protein 1 (DIP1), Contributes to Cell Fate Decisions during Drosophila Development J. Biol. Chem., September 26, 2003; 278(39): 38040 - 38050. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. W. Krauss, C. Chen, S. Penman, and R. Heald Nuclear actin and protein 4.1: Essential interactions during nuclear assembly in vitro PNAS, September 16, 2003; 100(19): 10752 - 10757. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Parra, S. L. Gee, M. J. Koury, N. Mohandas, and J. G. Conboy Alternative 5' exons and differential splicing regulate expression of protein 4.1R isoforms with distinct N-termini Blood, May 15, 2003; 101(10): 4164 - 4171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Luque, C. M. Perez-Ferreiro, A. Perez-Gonzalez, L. Englmeier, M. D. Koffa, and I. Correas An Alternative Domain Containing a Leucine-rich Sequence Regulates Nuclear Cytoplasmic Localization of Protein 4.1R J. Biol. Chem., January 17, 2003; 278(4): 2686 - 2691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. W. Krauss, R. Heald, G. Lee, W. Nunomura, J. A. Gimm, N. Mohandas, and J. A. Chasis Two Distinct Domains of Protein 4.1 Critical for Assembly of Functional Nuclei in Vitro J. Biol. Chem., November 8, 2002; 277(46): 44339 - 44346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kressel and B. Schmucker Nucleocytoplasmic transfer of the NF2 tumor suppressor protein merlin is regulated by exon 2 and a CRM1-dependent nuclear export signal in exon 15 Hum. Mol. Genet., September 15, 2002; 11(19): 2269 - 2278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Jones, J. Machado Jr., and A. Merlo Loss of Focal Adhesion Kinase (FAK) Inhibits Epidermal Growth Factor Receptor-dependent Migration and Induces Aggregation of NH2-Terminal FAK in the Nuclei of Apoptotic Glioblastoma Cells Cancer Res., July 1, 2001; 61(13): 4978 - 4981. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. D'Agostino, T. Ferro, L. Zotti, F. Meggio, L. A. Pinna, L. Chieco-Bianchi, and V. Ciminale Identification of a Domain in Human Immunodeficiency Virus Type 1 Rev That Is Required for Functional Activity and Modulates Association with Subnuclear Compartments Containing Splicing Factor SC35 J. Virol., December 15, 2000; 74(24): 11899 - 11910. [Abstract] [Full Text]
|
|
|
|
|
|
M. Lallena, C Martinez, J Valcarcel, and I Correas Functional association of nuclear protein 4.1 with pre-mRNA splicing factors J. Cell Sci., June 8, 2000; 111(14): 1963 - 1971. [Abstract] [PDF]
|
|
|
|
|
|
C. Luque and I Correas A constitutive region is responsible for nuclear targeting of 4.1R: modulation by alternative sequences results in differential intracellular localization J. Cell Sci., January 7, 2000; 113(13): 2485 - 2495. [Abstract] [PDF]
|
|
|
|
|
|
C. M. Luque, M.-J. Lallena, C. M. Perez-Ferreiro, Y. de Isidro, G. De Carcer, M. A. Alonso, and I. Correas The N-terminal 209-aa domain of high molecular- weight 4.1R isoforms abrogates 4.1R targeting to the nucleus PNAS, December 21, 1999; 96(26): 14925 - 14930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Gascard, W. Nunomura, G. Lee, L. D. Walensky, S. W. Krauss, Y. Takakuwa, J. A. Chasis, N. Mohandas, and J. G. Conboy Deciphering the Nuclear Import Pathway for the Cytoskeletal Red Cell Protein 4.1R Mol. Biol. Cell, June 1, 1999; 10(6): 1783 - 1798. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. N. Mattagajasingh, S.-C. Huang, J. S. Hartenstein, M. Snyder, V. T. Marchesi, and E. J. Benz Jr. A Nonerythroid Isoform of Protein 4.1R Interacts with the Nuclear Mitotic Apparatus (NuMA) Protein J. Cell Biol., April 5, 1999; 145(1): 29 - 43. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Gascard, G. Lee, L. Coulombel, I. Auffray, M. Lum, M. Parra, J.G. Conboy, N. Mohandas, and J.A. Chasis Characterization of Multiple Isoforms of Protein 4.1R Expressed During Erythroid Terminal Differentiation Blood, December 1, 1998; 92(11): 4404 - 4414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Luque, M.-J. Lallena, M. A. Alonso, and I. Correas An Alternative Domain Determines Nuclear Localization in Multifunctional Protein 4.1 J. Biol. Chem., May 8, 1998; 273(19): 11643 - 11649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Perez-Ferreiro, C. M. Luque, and I. Correas 4.1R Proteins Associate with Interphase Microtubules in Human T Cells. A 4.1R CONSTITUTIVE REGION IS INVOLVED IN TUBULIN BINDING J. Biol. Chem., November 21, 2001; 276(48): 44785 - 44791. [Abstract] [Full Text] [PDF]
|
|
