Journal of Cell Science, Vol 110, Issue 21 2715-2727, Copyright © 1997 by Company of Biologists

JOURNAL ARTICLES

Cloning of SEC61 homologues from Schizosaccharomyces pombe and Yarrowia lipolytica reveals the extent of functional conservation within this core component of the ER translocation machinery

J Broughton, D Swennen, BM Wilkinson, P Joyet, C Gaillardin and CJ Stirling
School of Biological Sciences, University of Manchester, UK.

The Sec61 protein is required for protein translocation across the ER membrane in both yeast and mammals and is found in close association with polypeptides during their membrane transit. In Saccharomyces cerevisiae Sec61p is essential for viability and the extent of sequence similarity between the yeast and mammalian proteins (55% sequence identity) suggests that the role of Sec61p in the translocation mechanism is likely to be conserved. In order to further our understanding of the structure and function of Sec61p we have cloned homologues from both Schizosaccharomyces pombe and Yarrowia lipolytica. The S. pombe gene comprises six exons encoding a 479 residue protein which we have immunolocalised to the endoplasmic reticulum. Sequence comparisons reveal that S. pombe Sec61p is 58.6% identical to that of S. cerevisiae. The deduced amino acid sequence of the Y. lipolytica protein shares 68.8% sequence identity with S. cerevisiae Sec61p. Gene disruption studies have shown that the SEC61 is required for viability in both S. pombe and Y. lipolytica demonstrating that the essential nature of this protein is not unique to S. cerevisiae. Moreover, heterologous complementation studies indicate that the Y. lipolytica SEC61 gene can complement a null mutation in S. cerevisiae. Sequence comparisons between the various eukaryotic Sec61p homologues reveal a number of highly conserved domains, including several transmembrane sequences and the majority of cytosolic loops. These comparisons will provide an important framework for the detailed analysis of interactions between Sec61p and other components of the translocation machinery and between Sec61p and translocating polypeptide chains.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				K. Hirakawa, S. Kobayashi, T. Inoue, S. Endoh-Yamagami, R. Fukuda, and A. Ohta Yas3p, an Opi1 Family Transcription Factor, Regulates Cytochrome P450 Expression in Response to n-Alkanes in Yarrowia lipolytica J. Biol. Chem., March 13, 2009; 284(11): 7126 - 7137. [Abstract] [Full Text] [PDF]

				A. Vjestica, X.-Z. Tang, and S. Oliferenko The Actomyosin Ring Recruits Early Secretory Compartments to the Division Site in Fission Yeast Mol. Biol. Cell, March 1, 2008; 19(3): 1125 - 1138. [Abstract] [Full Text] [PDF]

				A. Yan and W. J. Lennarz Two oligosaccharyl transferase complexes exist in yeast and associate with two different translocons Glycobiology, December 1, 2005; 15(12): 1407 - 1415. [Abstract] [Full Text] [PDF]

				J. L. Morrell-Falvey, L. Ren, A. Feoktistova, G. D. Haese, and K. L. Gould Cell wall remodeling at the fission yeast cell division site requires the Rho-GEF Rgf3p J. Cell Sci., December 1, 2005; 118(23): 5563 - 5573. [Abstract] [Full Text] [PDF]

				A. Boisrame, M. Chasles, A. Babour, J.-M. Beckerich, and C. Gaillardin Sbh1p, a subunit of the Sec61 translocon, interacts with the chaperone calnexin in the yeast Yarrowia lipolytica J. Cell Sci., March 14, 2003; 115(24): 4947 - 4956. [Abstract] [Full Text] [PDF]

				A. Decottignies, I. Sanchez-Perez, and P. Nurse Schizosaccharomyces pombe Essential Genes: A Pilot Study Genome Res., March 1, 2003; 13(3): 399 - 406. [Abstract] [Full Text] [PDF]

				Y. Tange, A. Hirata, and O. Niwa An evolutionarily conserved fission yeast protein, Ned1, implicated in normal nuclear morphology and chromosome stability, interacts with Dis3, Pim1/RCC1 and an essential nucleoporin J. Cell Sci., November 15, 2002; 115(22): 4375 - 4385. [Abstract] [Full Text] [PDF]

				A. L. O. Facanha, H. Appelgren, M. Tabish, L. Okorokov, and K. Ekwall The endoplasmic reticulum cation P-type ATPase Cta4p is required for control of cell shape and microtubule dynamics J. Cell Biol., June 10, 2002; 157(6): 1029 - 1040. [Abstract] [Full Text] [PDF]

				P Martinez and P. Ljungdahl The SHR3 homologue from S. pombe demonstrates a conserved function of ER packaging chaperones J. Cell Sci., January 12, 2000; 113(23): 4351 - 4362. [Abstract] [PDF]

				B. M. Wilkinson, J. R. Tyson, P. J. Reid, and C. J. Stirling Distinct Domains within Yeast Sec61p Involved in Post-translational Translocation and Protein Dislocation J. Biol. Chem., January 7, 2000; 275(1): 521 - 529. [Abstract] [Full Text] [PDF]