Journal of Cell Science, Vol 110, Issue 23 2935-2941, Copyright © 1997 by Company of Biologists

JOURNAL ARTICLES

HSF1 transcription factor concentrates in nuclear foci during heat shock: relationship with transcription sites

C Jolly, R Morimoto, M Robert-Nicoud and C Vourc'h
DyOGen, INSERM U309, Institut Albert Bonniot, Domaine de la Merci, 38706, La Tronche cedex, France. caroline.jolly@ujf-grenoble.fr

In this paper, we show that upon heat shock, HSF1 concentrates in the nucleus of diploid human fibroblasts in two large foci. The relative distribution of HSF1 nuclear foci and active heat shock protein (hsp) genes was investigated by combining fluorescence in situ hybridization (FISH) for the detection of hsp nuclear transcripts and immunofluorescence for the detection of HSF1. We show that the HSF1 foci are distinct from the sites of hsp70 and hsp90 genes transcription. This is the second report of ploidy-dependent foci of transcription factors that are independent of their specific transcription sites. However, the correlation between the number of HSF1 foci and the ploidy of the cells strongly supports the existence of a specific chromosomal target for HSF1 foci.

This article has been cited by other articles:

				R. Valgardsdottir, I. Chiodi, M. Giordano, A. Rossi, S. Bazzini, C. Ghigna, S. Riva, and G. Biamonti Transcription of Satellite III non-coding RNAs is a general stress response in human cells Nucleic Acids Res., February 2, 2008; 36(2): 423 - 434. [Abstract] [Full Text] [PDF]

				A. Shabtay and Z. Arad Reciprocal activation of HSF1 and HSF3 in brain and blood tissues: is redundancy developmentally related? Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2006; 291(3): R566 - R572. [Abstract] [Full Text] [PDF]

				S. D. Westerheide and R. I. Morimoto Heat Shock Response Modulators as Therapeutic Tools for Diseases of Protein Conformation J. Biol. Chem., September 30, 2005; 280(39): 33097 - 33100. [Abstract] [Full Text] [PDF]

				M. Nissinen, T. Kaisto, P. Salmela, J. Peltonen, and K. Metsikko Restricted Distribution of mRNAs Encoding a Sarcoplasmic Reticulum or Transverse Tubule Protein in Skeletal Myofibers J. Histochem. Cytochem., February 1, 2005; 53(2): 217 - 227. [Abstract] [Full Text] [PDF]

				A. Metz, J. Soret, C. Vourc'h, J. Tazi, and C. Jolly A key role for stress-induced satellite III transcripts in the relocalization of splicing factors into nuclear stress granules J. Cell Sci., September 1, 2004; 117(19): 4551 - 4558. [Abstract] [Full Text] [PDF]

				I. Chiodi, M. Corioni, M. Giordano, R. Valgardsdottir, C. Ghigna, F. Cobianchi, R.-M. Xu, S. Riva, and G. Biamonti RNA recognition motif 2 directs the recruitment of SF2/ASF to nuclear stress bodies Nucleic Acids Res., August 9, 2004; 32(14): 4127 - 4136. [Abstract] [Full Text] [PDF]

				C. Jolly, A. Metz, J. Govin, M. Vigneron, B. M. Turner, S. Khochbin, and C. Vourc'h Stress-induced transcription of satellite III repeats J. Cell Biol., January 5, 2004; 164(1): 25 - 33. [Abstract] [Full Text] [PDF]

				A. Sandqvist and L. Sistonen Nuclear stress granules: the awakening of a sleeping beauty? J. Cell Biol., January 5, 2004; 164(1): 15 - 17. [Abstract] [Full Text] [PDF]

				T.-P. Alastalo, M. Hellesuo, A. Sandqvist, V. Hietakangas, M. Kallio, and L. Sistonen Formation of nuclear stress granules involves HSF2 and coincides with the nucleolar localization of Hsp70 J. Cell Sci., September 1, 2003; 116(17): 3557 - 3570. [Abstract] [Full Text] [PDF]

				V. Hietakangas, J. K. Ahlskog, A. M. Jakobsson, M. Hellesuo, N. M. Sahlberg, C. I. Holmberg, A. Mikhailov, J. J. Palvimo, L. Pirkkala, and L. Sistonen Phosphorylation of Serine 303 Is a Prerequisite for the Stress-Inducible SUMO Modification of Heat Shock Factor 1 Mol. Cell. Biol., April 15, 2003; 23(8): 2953 - 2968. [Abstract] [Full Text] [PDF]

				M. Denegri, D. Moralli, M. Rocchi, M. Biggiogera, E. Raimondi, F. Cobianchi, L. De Carli, S. Riva, and G. Biamonti Human Chromosomes 9, 12, and 15 Contain the Nucleation Sites of Stress-Induced Nuclear Bodies Mol. Biol. Cell, June 1, 2002; 13(6): 2069 - 2079. [Abstract] [Full Text] [PDF]

				D. L. Spector Nuclear domains J. Cell Sci., March 10, 2002; 114(16): 2891 - 2893. [Full Text] [PDF]

				C. Jolly, L. Konecny, D. L. Grady, Y. A. Kutskova, J. J. Cotto, R. I. Morimoto, and C. Vourc'h In vivo binding of active heat shock transcription factor 1 to human chromosome 9 heterochromatin during stress J. Cell Biol., March 4, 2002; 156(5): 775 - 781. [Abstract] [Full Text] [PDF]

				J. A. Nielsen, L. D. Hudson, and R. C. Armstrong Nuclear organization in differentiating oligodendrocytes J. Cell Sci., January 11, 2002; 115(21): 4071 - 4079. [Abstract] [Full Text] [PDF]

				A. Mathew, S. K. Mathur, C. Jolly, S. G. Fox, S. Kim, and R. I. Morimoto Stress-Specific Activation and Repression of Heat Shock Factors 1 and 2 Mol. Cell. Biol., November 1, 2001; 21(21): 7163 - 7171. [Abstract] [Full Text] [PDF]

				M. Denegri, I. Chiodi, M. Corioni, F. Cobianchi, S. Riva, and G. Biamonti Stress-induced Nuclear Bodies Are Sites of Accumulation of Pre-mRNA Processing Factors Mol. Biol. Cell, November 1, 2001; 12(11): 3502 - 3514. [Abstract] [Full Text] [PDF]

				L. PIRKKALA, P. NYKANEN, and L. SISTONEN Roles of the heat shock transcription factors in regulation of the heat shock response and beyond FASEB J, May 1, 2001; 15(7): 1118 - 1131. [Abstract] [Full Text] [PDF]

				I Chiodi, M Biggiogera, M Denegri, M Corioni, F Weighardt, F Cobianchi, S Riva, and G Biamonti Structure and dynamics of hnRNP-labelled nuclear bodies induced by stress treatments J. Cell Sci., January 11, 2000; 113(22): 4043 - 4053. [Abstract] [PDF]

				C. Jolly, C. Vourc'h, M. Robert-Nicoud, and R. I. Morimoto Intron-independent Association of Splicing Factors with Active Genes J. Cell Biol., June 14, 1999; 145(6): 1133 - 1143. [Abstract] [Full Text] [PDF]

				C. Jolly, Y. Usson, and R. I. Morimoto Rapid and reversible relocalization of heat shock factor 1 within seconds to nuclear stress granules PNAS, June 8, 1999; 96(12): 6769 - 6774. [Abstract] [Full Text] [PDF]

				P. Mercier, N. Winegarden, and J. Westwood Human heat shock factor 1 is predominantly a nuclear protein before and after heat stress J. Cell Sci., January 8, 1999; 112(16): 2765 - 2774. [Abstract] [PDF]

				F Weighardt, F Cobianchi, L Cartegni, I Chiodi, A Villa, S Riva, and G Biamonti A novel hnRNP protein (HAP/SAF-B) enters a subset of hnRNP complexes and relocates in nuclear granules in response to heat shock J. Cell Sci., January 5, 1999; 112(10): 1465 - 1476. [Abstract] [PDF]

				R. I. Morimoto Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators Genes & Dev., December 15, 1998; 12(24): 3788 - 3796. [Full Text]

				J Cotto, S Fox, and R Morimoto HSF1 granules: a novel stress-induced nuclear compartment of human cells J. Cell Sci., January 12, 1997; 110(23): 2925 - 2934. [Abstract] [PDF]

				M. L. Goodson, Y. Hong, R. Rogers, M. J. Matunis, O.-K. Park-Sarge, and K. D. Sarge SUMO-1 Modification Regulates the DNA Binding Activity of Heat Shock Transcription Factor 2, a Promyelocytic Leukemia Nuclear Body Associated Transcription Factor J. Biol. Chem., May 18, 2001; 276(21): 18513 - 18518. [Abstract] [Full Text] [PDF]

				C. Jolly, L. Konecny, D. L. Grady, Y. A. Kutskova, J. J. Cotto, R. I. Morimoto, and C. Vourc'h In vivo binding of active heat shock transcription factor 1 to human chromosome 9 heterochromatin during stress J. Cell Biol., March 4, 2002; 156(5): 775 - 781. [Abstract] [Full Text] [PDF]