|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 110, Issue 23 2987-2999, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
V Del Priore, C Heath, C Snay, A MacMillan, L Gorsch, S Dagher and C Cole
Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755, USA.
Rat7p/Nup159p is an essential nucleoporin of Sac-charomyces cerevisiae originally isolated in a genetic screen designed to identify yeast temperature-sensitive mutants defective in mRNA export. Here we describe a detailed structural-functional analysis of Rat7p/Nup159p. The mutation in the rat7-1 ts allele, isolated in the original genetic screen, was found to be a single base pair change that created a stop codon approximately 100 amino acids upstream of the actual stop codon of this 1,460 amino acid polypeptide, thus eliminating one of the two predicted coiled-coil regions located near the carboxyl terminus of the protein. These coiled-coil regions are essential since an allele lacking both coiled-coil regions was unable to support growth under any conditions. In contrast, no other region of the protein was absolutely required. The SAFG/PSFG repeat region in the central third of the protein was completely dispensable for growth at temperatures between 16 degrees C and 37 degrees C and cells expressing this mutant allele were indistinguishable from wild type. Deletion of the amino-terminal third of the protein, upstream from the repeat region, or the portion between the repeat region and the coiled-coils resulted in temperature-sensitivity, but the two alleles showed distinct phenotypes with respect to the behavior of nuclear pore complexes (NPCs). Taken together, our data suggest that Rat7p/Nup159p is anchored within the NPC through its coiled-coil region and adjacent sequences. In addition, we postulate that the N-terminal third of Rat7p/Nup159p plays an important role in mRNA export.
This article has been cited by other articles:
|
|
 |
|
  M. West, J. B. Hedges, K.-Y. Lo, and A. W. Johnson Novel Interaction of the 60S Ribosomal Subunit Export Adapter Nmd3 at the Nuclear Pore Complex J. Biol. Chem., May 11, 2007; 282(19): 14028 - 14037. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Rollenhagen, C. A. Hodge, and C. N. Cole Following Temperature Stress, Export of Heat Shock mRNA Occurs Efficiently in Cells with Mutations in Genes Normally Important for mRNA Export Eukaryot. Cell, March 1, 2007; 6(3): 505 - 513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P. Denning and M. F. Rexach Rapid Evolution Exposes the Boundaries of Domain Structure and Function in Natively Unfolded FG Nucleoporins Mol. Cell. Proteomics, February 1, 2007; 6(2): 272 - 282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bernad, D. Engelsma, H. Sanderson, H. Pickersgill, and M. Fornerod Nup214-Nup88 Nucleoporin Subcomplex Is Required for CRM1-mediated 60 S Preribosomal Nuclear Export J. Biol. Chem., July 14, 2006; 281(28): 19378 - 19386. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. D. Belanger, A. Gupta, K. M. MacDonald, C. M. Ott, C. A. Hodge, C. M. Cole, and L. I. Davis Nuclear Pore Complex Function in Saccharomyces cerevisiae Is Influenced by Glycosylation of the Transmembrane Nucleoporin Pom152p Genetics, November 1, 2005; 171(3): 935 - 947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Estruch, C. A. Hodge, S. Rodriguez-Navarro, and C. N. Cole Physical and Genetic Interactions Link the Yeast Protein Zds1p with mRNA Nuclear Export J. Biol. Chem., March 11, 2005; 280(10): 9691 - 9697. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Rollenhagen, C. A. Hodge, and C. N. Cole The Nuclear Pore Complex and the DEAD Box Protein Rat8p/Dbp5p Have Nonessential Features Which Appear To Facilitate mRNA Export following Heat Shock Mol. Cell. Biol., June 1, 2004; 24(11): 4869 - 4879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Gao, N. Sumanaweera, S. M. Bailer, and U. Stochaj Nuclear Accumulation of the Small GTPase Gsp1p Depends on Nucleoporins Nup133p, Rat2p/Nup120p, Nup85p, Nic96p, and the Acetyl-CoA Carboxylase Acc1p J. Biol. Chem., July 3, 2003; 278(28): 25331 - 25340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P. Denning, S. S. Patel, V. Uversky, A. L. Fink, and M. Rexach Disorder in the nuclear pore complex: The FG repeat regions of nucleoporins are natively unfolded PNAS, March 4, 2003; 100(5): 2450 - 2455. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. C. Walther, H. S. Pickersgill, V. C. Cordes, M. W. Goldberg, T. D. Allen, I. W. Mattaj, and M. Fornerod The cytoplasmic filaments of the nuclear pore complex are dispensable for selective nuclear protein import J. Cell Biol., July 8, 2002; 158(1): 63 - 77. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-E. Gleizes, J. Noaillac-Depeyre, I. Leger-Silvestre, F. Teulieres, J.-Y. Dauxois, D. Pommet, M.-C. Azum-Gelade, and N. Gas Ultrastructural localization of rRNA shows defective nuclear export of preribosomes in mutants of the Nup82p complex J. Cell Biol., December 10, 2001; 155(6): 923 - 936. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Bailer, C. Balduf, and E. Hurt The Nsp1p Carboxy-Terminal Domain Is Organized into Functionally Distinct Coiled-Coil Regions Required for Assembly of Nucleoporin Subcomplexes and Nucleocytoplasmic Transport Mol. Cell. Biol., December 1, 2001; 21(23): 7944 - 7955. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Dilworth, A. Suprapto, J. C. Padovan, B. T. Chait, R. W. Wozniak, M. P. Rout, and J. D. Aitchison Nup2p Dynamically Associates with the Distal Regions of the Yeast Nuclear Pore Complex J. Cell Biol., June 25, 2001; 153(7): 1465 - 1478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Stra{beta}er, J. Ba{beta}ler, and E. Hurt Binding of the Mex67p/Mtr2p Heterodimer to FXFG, GLFG, and FG Repeat Nucleoporins Is Essential for Nuclear mRNA Export J. Cell Biol., August 21, 2000; 150(4): 695 - 706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Das, Z. Guo, P. Russo, P. Chartrand, and F. Sherman The Role of Nuclear Cap Binding Protein Cbc1p of Yeast in mRNA Termination and Degradation Mol. Cell. Biol., April 15, 2000; 20(8): 2827 - 2838. [Abstract] [Full Text]
|
|
|
|
|
|
C. Strambio-de-Castillia, G. Blobel, and M. P. Rout Proteins Connecting the Nuclear Pore Complex with the Nuclear Interior J. Cell Biol., March 8, 1999; 144(5): 839 - 855. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Seedorf, M. Damelin, J. Kahana, T. Taura, and P. A. Silver Interactions between a Nuclear Transporter and a Subset of Nuclear Pore Complex Proteins Depend on Ran GTPase Mol. Cell. Biol., February 1, 1999; 19(2): 1547 - 1557. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Belgareh, C. Snay-Hodge, F. Pasteau, S. Dagher, C. N. Cole, and V. Doye Functional Characterization of a Nup159p-containing Nuclear Pore Subcomplex Mol. Biol. Cell, December 1, 1998; 9(12): 3475 - 3492. [Abstract] [Full Text]
|
|
|
|
|
|
S. M. Bailer, C. Balduf, J. Katahira, A. Podtelejnikov, C. Rollenhagen, M. Mann, N. Pante, and E. Hurt Nup116p Associates with the Nup82p-Nsp1p-Nup159p Nucleoporin Complex J. Biol. Chem., July 28, 2000; 275(31): 23540 - 23548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Rout and J. D. Aitchison The Nuclear Pore Complex as a Transport Machine J. Biol. Chem., May 11, 2001; 276(20): 16593 - 16596. [Full Text] [PDF]
|
|
