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Journal of Cell Science, Vol 110, Issue 3 345-356, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
H Chen, NE Paradies, M Fedor-Chaiken and R Brackenbury
Department of Cell Biology, Neurobiology and Anatomy, University of Cincinnati College of Medicine, Ohio 45267-0521, USA.
Expression of the calcium-dependent adhesion molecule E-cadherin suppresses the invasion of cells in vitro, but the mechanism of this effect is unknown. To investigate this mechanism, we analyzed the effects of expressing E-cadherin in mouse L-cells and rat astrocyte-like WC5 cells. Increased cellular adhesion mediated by E-cadherin reduced invasion in WC5 cells and in some L-cells, but not in others. In all cases, suppression of invasion was correlated with decreased cell movement as assessed in an in vitro wound-filling assay and a transwell motility assay. To define the relationship between adhesion mediated by E-cadherin and suppression of motility, we analyzed the effects of deleting different regions of the E-cadherin cytoplasmic domain. E-cadherin lacking the entire cytoplasmic domain did not mediate calcium-dependent adhesion and did not reduce cell motility when expressed in WC5 cells. E-cadherin lacking a portion of the catenin-binding domain did not associate with the cytoskeleton and did not promote adhesion, yet still suppressed the motility of WC5 cells. In addition, E-cadherin that retains an intact catenin-binding domain, but lacks a juxtamembrane portion of the cytoplasmic domain, mediated effective adhesion, but did not suppress motility. These results indicate E-cadherin mediates adhesion and suppresses cell motility via distinct of E-cadherin plays a key role in suppressing motility.
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|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
S. Aono, S. Nakagawa, A. B. Reynolds, and M. Takeichi p120ctn Acts as an Inhibitory Regulator of Cadherin Function in Colon Carcinoma Cells J. Cell Biol., May 3, 1999; 145(3): 551 - 562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Daniel and A. B. Reynolds The Catenin p120ctn Interacts with Kaiso, a Novel BTB/POZ Domain Zinc Finger Transcription Factor Mol. Cell. Biol., May 1, 1999; 19(5): 3614 - 3623. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Pötter, C. Bergwitz, and G. Brabant The Cadherin-Catenin System: Implications for Growth and Differentiation of Endocrine Tissues Endocr. Rev., April 1, 1999; 20(2): 207 - 239. [Abstract] [Full Text]
|
|
|
|
 |
|
 A.I. Rojas and A.R. Ahmed Adhesion Receptors in Health and Disease Critical Reviews in Oral Biology & Medicine, January 1, 1999; 10(3): 337 - 358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
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W. Baumgartner, P. Hinterdorfer, W. Ness, A. Raab, D. Vestweber, H. Schindler, and D. Drenckhahn Cadherin interaction probed by atomic force microscopy PNAS, April 11, 2000; 97(8): 4005 - 4010. [Abstract] [Full Text] [PDF]
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K. Horikawa and M. Takeichi Requirement of the juxtamembrane domain of the cadherin cytoplasmic tail for morphogenetic cell rearrangement during myotome development J. Cell Biol., December 24, 2001; 155(7): 1297 - 1306. [Abstract] [Full Text] [PDF]
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