|
|
|
|
|
||
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
Journal of Cell Science, Vol 110, Issue 4 489-495, Copyright © 1997 by Company of Biologists
JOURNAL ARTICLES |
I Guenal, Y Risler and B Mignotte
Centre de Genetique Moleculaire, UPR 9061 du CNRS, Gif-sur-Yvette, France.
Inactivation of Simian Virus 40 large T antigen, in cells immortalized with conditional mutants, leads to activation of p53 and apoptosis. We used the mRNA differential display method to identify genes differentially expressed during this process. We found that steady-state levels of mRNA for cytoplasmic actins decreased early during apoptosis. We also showed that, although the steady-state level of the corresponding proteins is not profoundly affected, they are substrates for an interleukin 1-beta converting enzyme (ICE)-like protease activated during the process. However, only a very small fraction of actin is proteolysed during the early stages of apoptosis. The microfilament network is affected and non polymerized actin accumulates in apoptotic bodies after the decrease of mRNA levels, but before a significant amount of actin is cleaved. This suggests that down-regulation of actin genes may be involved in microfilament rearrangements during p53-mediated apoptosis.
This article has been cited by other articles:
|
|
 |
|
  J.-P. Levraud, M. Adam, M.-F. Luciani, C. de Chastellier, R. L. Blanton, and P. Golstein Dictyostelium cell death: early emergence and demise of highly polarized paddle cells J. Cell Biol., March 31, 2003; 160(7): 1105 - 1114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Staiger and V. E. Franklin-Tong The actin cytoskeleton is a target of the self-incompatibility response in Papaver rhoeas J. Exp. Bot., January 1, 2003; 54(380): 103 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Sahai p53 Moves Into Control of Cell Morphology Mol. Interv., September 1, 2002; 2(5): 286 - 289. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. White, P. Williams, K. R. Wojcik, S. Sun, P. S. Hiemstra, K. F. Rabe, and D. R. Dorscheid Initiation of Apoptosis by Actin Cytoskeletal Derangement in Human Airway Epithelial Cells Am. J. Respir. Cell Mol. Biol., March 1, 2001; 24(3): 282 - 294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Tezel and M. B. Wax The Mechanisms of hsp27 Antibody-Mediated Apoptosis in Retinal Neuronal Cells J. Neurosci., May 15, 2000; 20(10): 3552 - 3562. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W Bursch, K Hochegger, L Torok, B Marian, A Ellinger, and R. Hermann Autophagic and apoptotic types of programmed cell death exhibit different fates of cytoskeletal filaments J. Cell Sci., January 4, 2000; 113(7): 1189 - 1198. [Abstract] [PDF]
|
|
|
|
 |
|
 P. K. Davis and G. V. W. Johnson The Microtubule Binding of Tau and High Molecular Weight Tau in Apoptotic PC12 Cells Is Impaired because of Altered Phosphorylation J. Biol. Chem., December 10, 1999; 274(50): 35686 - 35692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Dinsdale, J. Zhuang, and G. M. Cohen Redistribution of Cytochrome c Precedes the Caspase-Dependent Formation of Ultracondensed Mitochondria, with a Reduced Inner Membrane Potential, in Apoptotic Monocytes Am. J. Pathol., August 1, 1999; 155(2): 607 - 618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Posey and B. E. Bierer Actin Stabilization by Jasplakinolide Enhances Apoptosis Induced by Cytokine Deprivation J. Biol. Chem., February 12, 1999; 274(7): 4259 - 4265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. J. Hwang, J. M. Ford, P. C. Hanawalt, and G. Chu Expression of the p48 xeroderma pigmentosum gene is p53-dependent and is involved in global genomic repair PNAS, January 19, 1999; 96(2): 424 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I Korichneva and U Hammerling F-actin as a functional target for retro-retinoids: a potential role in anhydroretinol-triggered cell death J. Cell Sci., January 8, 1999; 112(15): 2521 - 2528. [Abstract] [PDF]
|
|
